YTHDC1 (NM_133370) Human Tagged ORF Clone

CAT#: RG208796

YTHDC1 (GFP-tagged) - Human YTH domain containing 1 (YTHDC1), transcript variant 2. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: YTHDC1
• Synonyms: YT521; YT521-B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208796 ORF sequence, codon optimized.
  Due to the complexity of NM_133370, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGATTCAAGAGAGGAAAAAGATGGGGAACTTAACGTTCTGGACGACATACTGACGGAGGTAC
  CCGAGCAAGACGACGAACTGTACAACCCTGAGTCCGAGCAGGACAAAAATGAAAAGAAGGGGTCCAAAAG
  GAAGTCTGACAGAATGGAGTCCACCGATACCAAAAGACAGAAGCCTAGTGTGCACTCTAGACAACTGGTA
  TCCAAGCCCCTGAGCTCATCTGTTTCAAACAATAAAAGAATCGTGTCCACTAAGGGAAAGAGTGCCACCG
  AGTACAAAAACGAAGAGTATCAGCGGAGTGAGCGGAATAAGCGGCTCGACGCTGACAGAAAGATTAGACT
  GTCCAGCAGCGCTAGCAGAGAACCTTACAAAAATCAGCCTGAAAAAACATGCGTGAGGAAGCGGGACCCG
  GAGCGGAGAGCGAAAAGTCCCACCCCAGATGGATCAGAGCGCATAGGCCTCGAGGTTGACAGAAGGGCCT
  CTCGGAGCTCACAGTCATCCAAAGAGGAAGTCAATTCCGAGGAATACGGGAGCGACCACGAGACTGGTTC
  CTCCGGCTCCTCTGACGAGCAGGGAAACAACACCGAGAACGAGGAGGAGGGGGTGGAGGAGGACGTTGAG
  GAAGATGAAGAGGTCGAGGAGGATGCAGAGGAAGACGAAGAAGTGGACGAAGACGGCGAGGAGGAGGAAG
  AAGAAGAAGAGGAGGAGGAAGAAGAAGAGGAAGAGGAAGAGGAAGAATATGAGCAGGATGAGCGCGACCA
  GAAGGAAGAAGGCAACGATTACGATACAAGATCAGAAGCATCCGATAGTGGGTCCGAGAGTGTCTCCTTC
  ACCGACGGCAGCGTGCGCTCCGGTTCTGGCACAGACGGATCTGACGAGAAGAAGAAGGAGCGAAAAAGAG
  CTCGCGGTATCAGCCCTATCGTGTTTGACCGCTCTGGAAGCAGCGCTAGTGAGAGTTATGCCGACCAGAC
  CTCAAAGCTGAAGTATGTCCTTCAGGATGCCCGCTTTTTTCTCATTAAGTCTAATAATCATGAAAACGTT
  TCCCTGGCGAAGGCAAAAGGAGTGTGGTCTACCCTGCCCGTGAATGAGAAAAAACTGAATCTCGCTTTCC
  GATCCGCCCGCAGCGTCATTCTGATATTCAGCGTTCGGGAGTCAGGTAAATTCCAAGGTTTCGCTAGACT
  TAGCTCCGAGTCTCACCATGGCGGCAGCCCTATCCATTGGGTGTTGCCCGCTGGAATGTCTGCTAAGATG
  TTGGGGGGTGTATTTAAGATCGATTGGATTTGCAGGCGGGAGCTCCCATTCACCAAATCAGCACATTTGA
  CTAATCCATGGAATGAACACAAACCCGTGAAGATAGGCCGGGACGGTCAAGAGATTGAACTGGAATGCGG
  TACTCAACTGTGCCTTCTGTTCCCTCCAGACGAGTCAATCGATTTGTATCAGGTAATCCACAAGATGAGG
  CATAAGAGACGAATGCACTCCCAGCCAAGATCCCGAGGTAGGCCTTCTCGAAGAGAACCAGTCAGAGATG
  TTGGTCGACGGCGCCCGGAGGACTACGACATCCATAACTCTCGGAAGAAGCCTCGCATCGACTACCCCCC
  CGAATTCCATCAGCGGCCAGGGTATCTGAAAGACCCACGATATCAGGAGGTGGACAGAAGATTCTCAGGT
  GTACGCCGCGATGTTTTTTTGAACGGAAGCTACAACGACTACGTACGCGAGTTCCACAATATGGGTCCTC
  CCCCTCCTTGGCAGGGGATGCCACCGTACCCAGGTATGGAACAACCACCACATCATCCCTACTATCAACA
  CCATGCTCCGCCACCTCAGGCCCATCCTCCATACTCTGGACATCATCCAGTACCTCACGAAGCGAGGTAT
  CGAGACAAGAGAGTCCACGACTACGACATGAGGGTCGATGACTTTCTCAGAAGGACACAAGCTGTGGTGA
  GCGGACGAAGAAGTCGGCCACGCGAGCGAGACAGAGAAAGGGAAAGGGACCGCCCACGGGACAACCGCCG
  GGACCGGGAACGCGACCGGGGTAGGGATAGGGAAAGGGAAAGGGAAAGATTGTGTGATCGGGACCGCGAT
  AGGGGGGAGCGGGGAAGATACAGGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208796 representing NM_133370
  Red=Cloning site Green=Tags(s)
  
  MAADSREEKDGELNVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRKSDRMESTDTKRQKPSVHSRQLV
  SKPLSSSVSNNKRIVSTKGKSATEYKNEEYQRSERNKRLDADRKIRLSSSASREPYKNQPEKTCVRKRDP
  ERRAKSPTPDGSERIGLEVDRRASRSSQSSKEEVNSEEYGSDHETGSSGSSDEQGNNTENEEEGVEEDVE
  EDEEVEEDAEEDEEVDEDGEEEEEEEEEEEEEEEEEEEEYEQDERDQKEEGNDYDTRSEASDSGSESVSF
  TDGSVRSGSGTDGSDEKKKERKRARGISPIVFDRSGSSASESYADQTSKLKYVLQDARFFLIKSNNHENV
  SLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKM
  LGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQVIHKMR
  HKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDRRFSG
  VRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGMEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARY
  RDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERLCDRDRD
  RGERGRYRR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133370
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133370.2, NP_588611.2
• RefSeq Size: 6216
• RefSeq ORF: 2130
• Locus ID: 91746
• Domains: YTH