SREBP2 (SREBF2) (NM_004599) Human Tagged ORF Clone

CAT#: RG208942

SREBF2 (GFP-tagged) - Human sterol regulatory element binding transcription factor 2 (SREBF2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SREBF2
• Synonyms: bHLHd2; SREBP-2; SREBP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208942 representing NM_004599
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACAGCGGCGAGCTGGGTGGTCTGGAGACCATGGAGACCCTCACGGAGCTGGGCGACGAGCTGA
  CCCTGGGAGACATCGACGAGATGCTGCAATTTGTCAGTAATCAAGTGGGAGAGTTCCCTGACTTGTTTTC
  AGAACAGCTGTGTAGCTCCTTTCCTGGCAGTGGTGGTAGTGGTAGCAGCAGCGGCAGCAGTGGCAGCAGC
  AGCAGCAGCAGCAATGGCAGGGGCAGCAGCAGCGGAGCTGTGGACCCTTCAGTGCAACGGTCATTCACCC
  AGGTCACATTACCTTCCTTCTCTCCCTCGGCGGCCTCCCCACAGGCTCCAACTCTGCAAGTCAAGGTTTC
  TCCCACCTCAGTTCCCACCACACCCAGGGCAACTCCTATTCTTCAGCCCCGCCCCCAGCCCCAGCCTCAA
  CCTCAAACTCAGCTGCAACAACAGACGGTAATGATCACGCCAACATTCAGCACCACTCCGCAGACGAGGA
  TCATCCAGCAGCCTTTGATATACCAGAATGCAGCTACTAGCTTTCAAGTCCTTCAGCCTCAAGTCCAAAG
  CCTGGTGACATCCTCCCAGGTACAGCCGGTCACCATTCAGCAGCAGGTGCAGACAGTACAGGCCCAGCGG
  GTGCTGACACAAACGGCCAATGGCACGCTGCAGACCCTTGCCCCGGCTACGGTGCAGACAGTTGCTGCGC
  CACAGGTGCAGCAGGTCCCGGTCCTGGTCCAGCCTCAGATCATCAAGACAGATTCCCTTGTTTTGACCAC
  ACTGAAGACAGATGGCAGCCCTGTTATGGCTGCGGTCCAGAACCCGGCCCTCACCGCCCTCACCACCCCT
  ATCCAGACGGCTGCCCTTCAAGTACCAACCCTGGTGGGCAGCAGTGGGACCATTCTGACCACAATGCCTG
  TAATGATGGGGCAAGAGAAAGTGCCCATTAAGCAGGTACCTGGGGGAGTCAAGCAGCTTGAGCCCCCCAA
  AGAAGGAGAAAGGCGGACAACCCATAATATCATTGAGAAACGATATCGCTCCTCCATCAATGACAAAATC
  ATCGAATTGAAAGACCTGGTCATGGGGACAGACGCCAAGATGCACAAGTCTGGCGTTCTGAGGAAGGCCA
  TTGATTACATCAAATACTTGCAGCAGGTCAATCATAAACTGCGCCAGGAGAACATGGTGCTGAAGCTGGC
  AAATCAAAAGAACAAGCTTCTAAAGGGCATCGACCTAGGCAGTCTGGTGGACAATGAGGTGGACCTGAAG
  ATCGAGGACTTTAATCAGAATGTCCTTCTGATGTCCCCCCCAGCCTCTGACTCAGGGTCCCAGGCTGGCT
  TCTCTCCCTACTCCATTGACTCTGAGCCAGGAAGCCCTCTATTGGATGATGCAAAGGTCAAAGATGAGCC
  AGACTCTCCTCCTGTGGCGCTGGGCATGGTAGACCGCTCACGGATTCTTCTGTGTGTCCTCACCTTCCTG
  TGCCTCTCCTTTAACCCCCTGACTTCCCTGCTGCAGTGGGGAGGGGCCCACGACTCTGACCAGCACCCAC
  ACTCAGGCTCTGGCCGCAGTGTCCTGTCATTCGAGTCAGGTTCTGGGGGCTGGTTTGACTGGATGATGCC
  TACTCTTCTCTTATGGCTGGTAAATGGTGTGATTGTCCTGAGCGTCTTTGTGAAGCTTCTGGTTCATGGG
  GAGCCAGTGATCCGGCCACACTCGCGCTCCTCGGTCACCTTCTGGAGGCACCGGAAACAGGCAGATCTGG
  ATCTCGCCAGAGGAGATTTTGCAGCTGCTGCCGGCAACCTACAAACCTGCCTGGCAGTTTTGGGCCGGGC
  ACTGCCCACCTCCCGCCTGGACCTGGCCTGCAGCCTCTCCTGGAACGTGATCCGCTACAGCCTGCAGAAG
  CTACGCCTGGTGCGCTGGCTGCTCAAGAAAGTCTTCCAGTGCCGGCGGGCCACGCCAGCCACTGAGGCAG
  GCTTTGAAGACGAAGCTAAGACCAGCGCCCGGGATGCGGCTCTGGCCTATCACCGGCTGCACCAGCTGCA
  CATCACAGGGAAGCTTCCTGCAGGATCCGCCTGTTCCGATGTACACATGGCGTTGTGTGCCGTGAACCTG
  GCTGAATGTGCAGAGGAGAAGATCCCACCGAGCACACTGGTTGAGATCCATCTGACTGCTGCCATGGGGC
  TCAAGACCCGGTGTGGAGGCAAGCTGGGCTTCCTGGCCAGCTACTTCCTCAGCCGAGCCCAGAGCCTGTG
  TGGCCCCGAGCACAGTGCTGTTCCTGACTCCCTGCGCTGGCTCTGCCACCCCCTGGGCCAGAAGTTTTTC
  ATGGAGCGGAGCTGGTCTGTGAAGTCAGCTGCCAAGGAGAGTCTATACTGTGCCCAGAGGAACCCAGCTG
  ACCCCATTGCGCAGGTCCACCAGGCCTTCTGCAAGAACCTGCTGGAGCGAGCTATAGAGTCCTTGGTGAA
  ACCTCAGGCCAAGAAGAAGGCTGGAGACCAGGAAGAAGAGAGCTGTGAATTCTCCAGTGCTCTGGAGTAC
  TTGAAATTACTTCATTCTTTTGTGGACTCTGTGGGGGTTATGAGCCCCCCACTCTCCAGGAGCTCCGTGC
  TCAAGTCCGCCCTGGGTCCAGACATCATCTGTCGGTGGTGGACGTCTGCAATCACTGTGGCCATCAGCTG
  GCTCCAGGGAGACGATGCAGCTGTGCGCTCTCATTTTACCAAAGTGGAACGCATCCCCAAGGCCCTGGAA
  GTGACAGAGAGCCCCCTGGTGAAGGCCATCTTCCATGCCTGCAGAGCCATGCATGCCTCACTCCCTGGGA
  AAGCAGATGGGCAGCAGAGTTCCTTCTGCCATTGCGAGAGGGCCAGTGGCCACCTATGGAGCAGCCTCAA
  CGTCAGTGGGGCCACCTCTGACCCTGCCCTCAACCACGTGGTCCAGCTGCTCACCTGTGACCTGCTACTG
  TCGCTACGGACAGCGCTCTGGCAAAAACAGGCCAGTGCCAGCCAGGCTGTGGGGGAGACCTACCACGCGT
  CAGGCGCTGAACTGGCGGGCTTCCAACGGGACCTGGGCAGCCTGCGCAGGCTGGCACACAGCTTCCGCCC
  AGCATACCGCAAGGTGTTCCTGCATGAAGCCACCGTGCGCCTGATGGCAGGAGCCAGCCCCACCCGCACC
  CACCAGCTGCTGGAACACAGCCTGCGGCGGCGCACCACGCAGAGCACCAAGCACGGAGAGGTGGATGCCT
  GGCCCGGCCAGCGAGAGCGGGCCACCGCCATCCTGCTGGCCTGCCGCCACCTGCCCCTCTCCTTCCTCTC
  CTCCCCGGGCCAGCGGGCAGTGCTGCTGGCCGAAGCTGCCCGCACCCTGGAGAAGGTGGGCGACCGGCGC
  TCCTGCAACGACTGCCAGCAGATGATTGTTAAGCTGGGTGGTGGCACTGCCATTGCCGCCTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208942 representing NM_004599
  Red=Cloning site Green=Tags(s)
  
  MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSS
  SSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQ
  PQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQR
  VLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTP
  IQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKI
  IELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLK
  IEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFL
  CLSFNPLTSLLQWGGAHDSDQHPHSGSGRSVLSFESGSGGWFDWMMPTLLLWLVNGVIVLSVFVKLLVHG
  EPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAGNLQTCLAVLGRALPTSRLDLACSLSWNVIRYSLQK
  LRLVRWLLKKVFQCRRATPATEAGFEDEAKTSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNL
  AECAEEKIPPSTLVEIHLTAAMGLKTRCGGKLGFLASYFLSRAQSLCGPEHSAVPDSLRWLCHPLGQKFF
  MERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEY
  LKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALE
  VTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLL
  SLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRT
  HQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRR
  SCNDCQQMIVKLGGGTAIAAS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004599
• ORF Size: 3423 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004599.2, NP_004590.2
• RefSeq Size: 4325 bp
• RefSeq ORF: 3426 bp
• Locus ID: 6721
• Cytogenetics: 22q13.2
• Domains: HLH
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]'