Centaurin beta 2 (ACAP2) (NM_012287) Human Tagged ORF Clone

CAT#: RG209171

ACAP2 (GFP-tagged) - Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 (ACAP2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACAP2
• Synonyms: CENTB2; CNT-B2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209171 representing NM_012287
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGACTGTGGATTTCGAGGAGTGTCTGAAGGACTCGCCCCGCTTCAGGGCAGCTTTGGAAGAAG
  TAGAAGGTGATGTGGCAGAATTGGAACTAAAACTTGATAAGCTTGTGAAACTTTGTATTGCAATGATTGA
  TACTGGAAAAGCCTTTTGTGTTGCAAATAAACAGTTCATGAATGGGATTCGAGACCTGGCTCAGTATTCT
  AGTAATGATGCTGTCGTTGAGACAAGTTTGACCAAGTTTTCTGACAGTCTTCAAGAAATGATAAATTTTC
  ACACAATCCTGTTTGACCAAACTCAGAGATCAATTAAGGCACAGCTTCAGAACTTTGTTAAAGAAGATCT
  TAGAAAATTCAAAGATGCCAAGAAGCAATTCGAAAAAGTCAGTGAAGAAAAAGAAAATGCGTTAGTAAAA
  AATGCCCAAGTACAAAGAAACAAACAACATGAAGTTGAAGAAGCCACCAACATTCTGACAGCAACAAGAA
  AATGTTTCCGACACATAGCCCTCGATTATGTGCTTCAGATTAATGTTCTTCAATCAAAAAGGAGATCAGA
  AATCCTAAAATCAATGTTGTCATTTATGTATGCCCATTTGGCCTTCTTTCATCAAGGATATGATCTGTTT
  AGTGAACTTGGACCCTACATGAAGGATCTTGGTGCACAGTTGGATCGACTGGTTGTGGATGCAGCAAAGG
  AGAAAAGAGAAATGGAGCAAAAACATTCCACCATTCAACAAAAGGATTTCTCCAGTGATGATTCTAAGTT
  AGAATATAACGTAGATGCTGCAAATGGCATAGTTATGGAAGGATATCTGTTCAAACGAGCCAGCAATGCC
  TTCAAAACTTGGAACAGGCGCTGGTTTTCAATACAGAATAATCAGTTGGTTTACCAGAAAAAATTTAAGG
  ATAATCCGACTGTGGTAGTTGAAGACCTCAGGCTTTGCACAGTGAAACATTGTGAAGACATAGAGCGACG
  ATTCTGCTTTGAGGTGGTCTCGCCAACAAAAAGTTGCATGCTCCAGGCAGATTCCGAAAAGCTGCGCCAG
  GCATGGATTAAGGCTGTTCAGACCAGTATTGCTACTGCTTATAGAGAGAAGGGTGATGAATCAGAGAAGC
  TGGATAAGAAATCATCTCCATCCACAGGAAGCCTAGATTCTGGAAATGAGTCCAAAGAGAAATTATTGAA
  AGGAGAAAGTGCGCTTCAGCGGGTCCAGTGTATCCCTGGCAATGCCAGCTGTTGTGACTGTGGCCTGGCA
  GATCCACGGTGGGCCAGCATCAACCTGGGCATCACCTTGTGTATCGAGTGCTCCGGAATTCACCGGAGCC
  TTGGGGTTCATTTTTCAAAAGTACGATCTTTAACTTTAGACACCTGGGAGCCAGAACTTTTAAAGCTTAT
  GTGTGAGTTGGGGAATGATGTTATAAATCGAGTTTATGAAGCTAATGTGGAAAAAATGGGAATAAAGAAA
  CCCCAACCAGGACAAAGACAGGAGAAGGAGGCATATATCAGAGCAAAATATGTGGAGAGGAAATTTGTGG
  ATAAATATTCTATATCATTATCACCTCCTGAGCAGCAAAAAAAGTTTGTCTCTAAAAGTTCTGAAGAAAA
  GAGGCTGAGCATTTCTAAATTTGGGCCAGGGGACCAAGTCAGAGCATCTGCCCAAAGTTCAGTCAGAAGT
  AATGACAGTGAAATTCAGCAGAGCTCTGATGATGGAAGAGAATCTTTACCCTCCACGGTGTCAGCCAATA
  GTTTATATGAGCCTGAAGGAGAAAGGCAAGATTCTTCTATGTTTCTTGACTCGAAACATCTTAATCCAGG
  ACTTCAGCTTTATAGGGCGTCATATGAAAAAAACCTTCCTAAAATGGCTGAGGCTTTGGCTCATGGTGCA
  GACGTGAACTGGGCCAATTCAGAGGAAAACAAAGCGACGCCACTTATTCAGGCTGTATTAGGGGGCTCTT
  TGGTGACGTGTGAGTTCCTCCTACAGAATGGTGCTAATGTCAACCAAAGAGATGTCCAAGGGCGGGGACC
  ATTGCACCATGCCACCGTCTTAGGGCACACAGGGCAGGTATGTTTATTCCTAAAACGAGGTGCCAATCAA
  CATGCCACTGATGAAGAAGGGAAAGACCCTTTGAGCATAGCTGTGGAAGCAGCCAATGCTGATATAGTCA
  CCTTGTTACGTTTAGCAAGAATGAATGAAGAGATGCGGGAATCAGAAGGACTTTATGGACAGCCAGGTGA
  TGAAACTTATCAGGACATATTTCGTGATTTTTCCCAAATGGCATCCAATAATCCAGAGAAACTAAATCGT
  TTCCAGCAAGATTCACAGAAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209171 representing NM_012287
  Red=Cloning site Green=Tags(s)
  
  MKMTVDFEECLKDSPRFRAALEEVEGDVAELELKLDKLVKLCIAMIDTGKAFCVANKQFMNGIRDLAQYS
  SNDAVVETSLTKFSDSLQEMINFHTILFDQTQRSIKAQLQNFVKEDLRKFKDAKKQFEKVSEEKENALVK
  NAQVQRNKQHEVEEATNILTATRKCFRHIALDYVLQINVLQSKRRSEILKSMLSFMYAHLAFFHQGYDLF
  SELGPYMKDLGAQLDRLVVDAAKEKREMEQKHSTIQQKDFSSDDSKLEYNVDAANGIVMEGYLFKRASNA
  FKTWNRRWFSIQNNQLVYQKKFKDNPTVVVEDLRLCTVKHCEDIERRFCFEVVSPTKSCMLQADSEKLRQ
  AWIKAVQTSIATAYREKGDESEKLDKKSSPSTGSLDSGNESKEKLLKGESALQRVQCIPGNASCCDCGLA
  DPRWASINLGITLCIECSGIHRSLGVHFSKVRSLTLDTWEPELLKLMCELGNDVINRVYEANVEKMGIKK
  PQPGQRQEKEAYIRAKYVERKFVDKYSISLSPPEQQKKFVSKSSEEKRLSISKFGPGDQVRASAQSSVRS
  NDSEIQQSSDDGRESLPSTVSANSLYEPEGERQDSSMFLDSKHLNPGLQLYRASYEKNLPKMAEALAHGA
  DVNWANSEENKATPLIQAVLGGSLVTCEFLLQNGANVNQRDVQGRGPLHHATVLGHTGQVCLFLKRGANQ
  HATDEEGKDPLSIAVEAANADIVTLLRLARMNEEMRESEGLYGQPGDETYQDIFRDFSQMASNNPEKLNR
  FQQDSQKF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012287
• ORF Size: 2334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012287.3, NP_036419.2
• RefSeq Size: 7102
• RefSeq ORF: 2337
• Locus ID: 23527
• Domains: ArfGap, PH, ANK
• Protein Pathways: Endocytosis