Centaurin beta 2 (ACAP2) (NM_012287) Human Tagged ORF Clone

CAT#: RC209171

ACAP2 (Myc-DDK-tagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 (ACAP2)

Product Images

Western blot validation of overexpression lysate (Cat# LY415840) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209171 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ACAP2 protein (Cat# TP309171). The protein was produced from HEK293T cells transfected with ACAP2 cDNA clone (Cat# RC209171) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACAP2
• Synonyms: CENTB2; CNT-B2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209171 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGACTGTGGATTTCGAGGAGTGTCTGAAGGACTCGCCCCGCTTCAGGGCAGCTTTGGAAGAAG
  TAGAAGGTGATGTGGCAGAATTGGAACTAAAACTTGATAAGCTTGTGAAACTTTGTATTGCAATGATTGA
  TACTGGAAAAGCCTTTTGTGTTGCAAATAAACAGTTCATGAATGGGATTCGAGACCTGGCTCAGTATTCT
  AGTAATGATGCTGTCGTTGAGACAAGTTTGACCAAGTTTTCTGACAGTCTTCAAGAAATGATAAATTTTC
  ACACAATCCTGTTTGACCAAACTCAGAGATCAATTAAGGCACAGCTTCAGAACTTTGTTAAAGAAGATCT
  TAGAAAATTCAAAGATGCCAAGAAGCAATTCGAAAAAGTCAGTGAAGAAAAAGAAAATGCGTTAGTAAAA
  AATGCCCAAGTACAAAGAAACAAACAACATGAAGTTGAAGAAGCCACCAACATTCTGACAGCAACAAGAA
  AATGTTTCCGACACATAGCCCTCGATTATGTGCTTCAGATTAATGTTCTTCAATCAAAAAGGAGATCAGA
  AATCCTAAAATCAATGTTGTCATTTATGTATGCCCATTTGGCCTTCTTTCATCAAGGATATGATCTGTTT
  AGTGAACTTGGACCCTACATGAAGGATCTTGGTGCACAGTTGGATCGACTGGTTGTGGATGCAGCAAAGG
  AGAAAAGAGAAATGGAGCAAAAACATTCCACCATTCAACAAAAGGATTTCTCCAGTGATGATTCTAAGTT
  AGAATATAACGTAGATGCTGCAAATGGCATAGTTATGGAAGGATATCTGTTCAAACGAGCCAGCAATGCC
  TTCAAAACTTGGAACAGGCGCTGGTTTTCAATACAGAATAATCAGTTGGTTTACCAGAAAAAATTTAAGG
  ATAATCCGACTGTGGTAGTTGAAGACCTCAGGCTTTGCACAGTGAAACATTGTGAAGACATAGAGCGACG
  ATTCTGCTTTGAGGTGGTCTCGCCAACAAAAAGTTGCATGCTCCAGGCAGATTCCGAAAAGCTGCGCCAG
  GCATGGATTAAGGCTGTTCAGACCAGTATTGCTACTGCTTATAGAGAGAAGGGTGATGAATCAGAGAAGC
  TGGATAAGAAATCATCTCCATCCACAGGAAGCCTAGATTCTGGAAATGAGTCCAAAGAGAAATTATTGAA
  AGGAGAAAGTGCGCTTCAGCGGGTCCAGTGTATCCCTGGCAATGCCAGCTGTTGTGACTGTGGCCTGGCA
  GATCCACGGTGGGCCAGCATCAACCTGGGCATCACCTTGTGTATCGAGTGCTCCGGAATTCACCGGAGCC
  TTGGGGTTCATTTTTCAAAAGTACGATCTTTAACTTTAGACACCTGGGAGCCAGAACTTTTAAAGCTTAT
  GTGTGAGTTGGGGAATGATGTTATAAATCGAGTTTATGAAGCTAATGTGGAAAAAATGGGAATAAAGAAA
  CCCCAACCAGGACAAAGACAGGAGAAGGAGGCATATATCAGAGCAAAATATGTGGAGAGGAAATTTGTGG
  ATAAATATTCTATATCATTATCACCTCCTGAGCAGCAAAAAAAGTTTGTCTCTAAAAGTTCTGAAGAAAA
  GAGGCTGAGCATTTCTAAATTTGGGCCAGGGGACCAAGTCAGAGCATCTGCCCAAAGTTCAGTCAGAAGT
  AATGACAGTGAAATTCAGCAGAGCTCTGATGATGGAAGAGAATCTTTACCCTCCACGGTGTCAGCCAATA
  GTTTATATGAGCCTGAAGGAGAAAGGCAAGATTCTTCTATGTTTCTTGACTCGAAACATCTTAATCCAGG
  ACTTCAGCTTTATAGGGCGTCATATGAAAAAAACCTTCCTAAAATGGCTGAGGCTTTGGCTCATGGTGCA
  GACGTGAACTGGGCCAATTCAGAGGAAAACAAAGCGACGCCACTTATTCAGGCTGTATTAGGGGGCTCTT
  TGGTGACGTGTGAGTTCCTCCTACAGAATGGTGCTAATGTCAACCAAAGAGATGTCCAAGGGCGGGGACC
  ATTGCACCATGCCACCGTCTTAGGGCACACAGGGCAGGTATGTTTATTCCTAAAACGAGGTGCCAATCAA
  CATGCCACTGATGAAGAAGGGAAAGACCCTTTGAGCATAGCTGTGGAAGCAGCCAATGCTGATATAGTCA
  CCTTGTTACGTTTAGCAAGAATGAATGAAGAGATGCGGGAATCAGAAGGACTTTATGGACAGCCAGGTGA
  TGAAACTTATCAGGACATATTTCGTGATTTTTCCCAAATGGCATCCAATAATCCAGAGAAACTAAATCGT
  TTCCAGCAAGATTCACAGAAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209171 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKMTVDFEECLKDSPRFRAALEEVEGDVAELELKLDKLVKLCIAMIDTGKAFCVANKQFMNGIRDLAQYS
  SNDAVVETSLTKFSDSLQEMINFHTILFDQTQRSIKAQLQNFVKEDLRKFKDAKKQFEKVSEEKENALVK
  NAQVQRNKQHEVEEATNILTATRKCFRHIALDYVLQINVLQSKRRSEILKSMLSFMYAHLAFFHQGYDLF
  SELGPYMKDLGAQLDRLVVDAAKEKREMEQKHSTIQQKDFSSDDSKLEYNVDAANGIVMEGYLFKRASNA
  FKTWNRRWFSIQNNQLVYQKKFKDNPTVVVEDLRLCTVKHCEDIERRFCFEVVSPTKSCMLQADSEKLRQ
  AWIKAVQTSIATAYREKGDESEKLDKKSSPSTGSLDSGNESKEKLLKGESALQRVQCIPGNASCCDCGLA
  DPRWASINLGITLCIECSGIHRSLGVHFSKVRSLTLDTWEPELLKLMCELGNDVINRVYEANVEKMGIKK
  PQPGQRQEKEAYIRAKYVERKFVDKYSISLSPPEQQKKFVSKSSEEKRLSISKFGPGDQVRASAQSSVRS
  NDSEIQQSSDDGRESLPSTVSANSLYEPEGERQDSSMFLDSKHLNPGLQLYRASYEKNLPKMAEALAHGA
  DVNWANSEENKATPLIQAVLGGSLVTCEFLLQNGANVNQRDVQGRGPLHHATVLGHTGQVCLFLKRGANQ
  HATDEEGKDPLSIAVEAANADIVTLLRLARMNEEMRESEGLYGQPGDETYQDIFRDFSQMASNNPEKLNR
  FQQDSQKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012287
• ORF Size: 2334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012287.3, NM_012287.4, NM_012287.5, NP_036419.2, NP_036419.3
• RefSeq Size: 7187 bp
• RefSeq ORF: 2337 bp
• Locus ID: 23527
• Domains: ArfGap, PH, ANK
• Protein Pathways: Endocytosis
• MW: 88.1 kDa