eNOS (NOS3) (NM_000603) Human Tagged ORF Clone

CAT#: RG209228

NOS3 (GFP-tagged) - Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NOS3
• Synonyms: ECNOS; eNOS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209228 representing NM_000603
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACTTGAAGAGCGTGGCCCAGGAGCCTGGGCCACCCTGCGGCCTGGGGCTGGGGCTGGGCCTTG
  GGCTGTGCGGCAAGCAGGGCCCAGCCACCCCGGCCCCTGAGCCCAGCCGGGCCCCAGCATCCCTACTCCC
  ACCAGCGCCAGAACACAGCCCCCCGAGCTCCCCGCTAACCCAGCCCCCAGAGGGGCCCAAGTTCCCTCGT
  GTGAAGAACTGGGAGGTGGGGAGCATCACCTATGACACCCTCAGCGCCCAGGCGCAGCAGGATGGGCCCT
  GCACCCCAAGACGCTGCCTGGGCTCCCTGGTATTTCCACGGAAACTACAGGGCCGGCCCTCCCCCGGCCC
  CCCGGCCCCTGAGCAGCTGCTGAGTCAGGCCCGGGACTTCATCAACCAGTACTACAGCTCCATTAAGAGG
  AGCGGCTCCCAGGCCCACGAACAGCGGCTTCAAGAGGTGGAAGCCGAGGTGGCAGCCACAGGCACCTACC
  AGCTTAGGGAGAGCGAGCTGGTGTTCGGGGCTAAGCAGGCCTGGCGCAACGCTCCCCGCTGCGTGGGCCG
  GATCCAGTGGGGGAAGCTGCAGGTGTTCGATGCCCGGGACTGCAGGTCTGCACAGGAAATGTTCACCTAC
  ATCTGCAACCACATCAAGTATGCCACCAACCGGGGCAACCTTCGCTCGGCCATCACAGTGTTCCCGCAGC
  GCTGCCCTGGCCGAGGAGACTTCCGAATCTGGAACAGCCAGCTGGTGCGCTACGCGGGCTACCGGCAGCA
  GGATGGCTCTGTGCGGGGGGACCCAGCCAACGTGGAGATCACCGAGCTCTGCATTCAGCACGGCTGGACC
  CCAGGAAACGGTCGCTTCGACGTGCTGCCCCTGCTGCTGCAGGCCCCAGATGATCCCCCAGAACTCTTCC
  TTCTGCCCCCCGAGCTGGTCCTTGAGGTGCCCCTGGAGCACCCCACGCTGGAGTGGTTTGCAGCCCTGGG
  CCTGCGCTGGTACGCCCTCCCGGCAGTGTCCAACATGCTGCTGGAAATTGGGGGCCTGGAGTTCCCCGCA
  GCCCCCTTCAGTGGCTGGTACATGAGCACTGAGATCGGCACGAGGAACCTGTGTGACCCTCACCGCTACA
  ACATCCTGGAGGATGTGGCTGTCTGCATGGACCTGGATACCCGGACCACCTCGTCCCTGTGGAAAGACAA
  GGCAGCAGTGGAAATCAACGTGGCCGTGCTGCACAGTTACCAGCTAGCCAAAGTCACCATCGTGGACCAC
  CACGCCGCCACGGCCTCTTTCATGAAGCACCTGGAGAATGAGCAGAAGGCCAGGGGGGGCTGCCCTGCAG
  ACTGGGCCTGGATCGTGCCCCCCATCTCGGGCAGCCTCACTCCTGTTTTCCATCAGGAGATGGTCAACTA
  TTTCCTGTCCCCGGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGGAGTGCCGCCAAGGGCACCGGCATC
  ACCAGGAAGAAGACCTTTAAAGAAGTGGCCAACGCCGTGAAGATCTCCGCCTCGCTCATGGGCACGGTGA
  TGGCGAAGCGAGTGAAGGCGACAATCCTGTATGGCTCCGAGACCGGCCGGGCCCAGAGCTACGCACAGCA
  GCTGGGGAGACTCTTCCGGAAGGCTTTTGATCCCCGGGTCCTGTGTATGGATGAGTATGACGTGGTGTCC
  CTCGAACACGAGACGCTGGTGCTGGTGGTAACCAGCACATTTGGGAATGGGGATCCCCCGGAGAATGGAG
  AGAGCTTTGCAGCTGCCCTGATGGAGATGTCCGGCCCCTACAACAGCTCCCCTCGGCCGGAACAGCACAA
  GAGTTATAAGATCCGCTTCAACAGCATCTCCTGCTCAGACCCACTGGTGTCCTCTTGGCGGCGGAAGAGG
  AAGGAGTCCAGTAACACAGACAGTGCAGGGGCCCTGGGCACCCTCAGGTTCTGTGTGTTCGGGCTCGGCT
  CCCGGGCATACCCCCACTTCTGCGCCTTTGCTCGTGCCGTGGACACACGGCTGGAGGAACTGGGCGGGGA
  GCGGCTGCTGCAGCTGGGCCAGGGCGACGAGCTGTGCGGCCAGGAGGAGGCCTTCCGAGGCTGGGCCCAG
  GCTGCCTTCCAGGCCGCCTGTGAGACCTTCTGTGTGGGAGAGGATGCCAAGGCCGCCGCCCGAGACATCT
  TCAGCCCCAAACGGAGCTGGAAGCGCCAGAGGTACCGGCTGAGCGCCCAGGCCGAGGGCCTGCAGTTGCT
  GCCAGGTCTGATCCACGTGCACAGGCGGAAGATGTTCCAGGCTACAATCCGCTCAGTGGAAAACCTGCAA
  AGCAGCAAGTCCACGAGGGCCACCATCCTGGTGCGCCTGGACACCGGAGGCCAGGAGGGGCTGCAGTACC
  AGCCGGGGGACCACATAGGTGTCTGCCCGCCCAACCGGCCCGGCCTTGTGGAGGCGCTGCTGAGCCGCGT
  GGAGGACCCGCCGGCGCCCACTGAGCCCGTGGCAGTAGAGCAGCTGGAGAAGGGCAGCCCTGGTGGCCCT
  CCCCCCGGCTGGGTGCGGGACCCCCGGCTGCCCCCGTGCACGCTGCGCCAGGCTCTCACCTTCTTCCTGG
  ACATCACCTCCCCACCCAGCCCTCAGCTCTTGCGGCTGCTCAGCACCTTGGCAGAAGAGCCCAGGGAACA
  GCAGGAGCTGGAGGCCCTCAGCCAGGATCCCCGACGCTACGAGGAGTGGAAGTGGTTCCGCTGCCCCACG
  CTGCTGGAGGTGCTGGAGCAGTTCCCGTCGGTGGCGCTGCCTGCCCCACTGCTCCTCACCCAGCTGCCTC
  TGCTCCAGCCCCGGTACTACTCAGTCAGCTCGGCACCCAGCACCCACCCAGGAGAGATCCACCTCACTGT
  AGCTGTGCTGGCATACAGGACTCAGGATGGGCTGGGCCCCCTGCACTATGGAGTCTGCTCCACGTGGCTA
  AGCCAGCTCAAGCCCGGAGACCCTGTGCCCTGCTTCATCCGGGGGGCTCCCTCCTTCCGGCTGCCACCCG
  ATCCCAGCTTGCCCTGCATCCTGGTGGGTCCAGGCACTGGCATTGCCCCCTTCCGGGGATTCTGGCAGGA
  GCGGCTGCATGACATTGAGAGCAAAGGGCTGCAGCCCACTCCCATGACTTTGGTGTTCGGCTGCCGATGC
  TCCCAACTTGACCATCTCTACCGCGACGAGGTGCAGAACGCCCAGCAGCGCGGGGTGTTTGGCCGAGTCC
  TCACCGCCTTCTCCCGGGAACCTGACAACCCCAAGACCTACGTGCAGGACATCCTGAGGACGGAGCTGGC
  TGCGGAGGTGCACCGCGTGCTGTGCCTCGAGCGGGGCCACATGTTTGTCTGCGGCGATGTTACCATGGCA
  ACCAACGTCCTGCAGACCGTGCAGCGCATCCTGGCGACGGAGGGCGACATGGAGCTGGACGAGGCCGGCG
  ACGTCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAAGACATTTTCGGGCTCACGCTGCGCACCCA
  GGAGGTGACAAGCCGCATACGCACCCAGAGCTTTTCCTTGCAGGAGCGTCAGTTGCGGGGCGCAGTGCCC
  TGGGCGTTCGACCCTCCCGGCTCAGACACCAACAGCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209228 representing NM_000603
  Red=Cloning site Green=Tags(s)
  
  MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPR
  VKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKR
  SGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTY
  ICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWT
  PGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPA
  APFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDH
  HAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGI
  TRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVS
  LEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKR
  KESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQ
  AAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQ
  SSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGP
  PPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPT
  LLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWL
  SQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRC
  SQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMA
  TNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVP
  WAFDPPGSDTNSP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000603
• ORF Size: 3609 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000603.3, NP_000594.2
• RefSeq Size: 4341 bp
• RefSeq ORF: 3612 bp
• Locus ID: 4846
• Cytogenetics: 7q36.1
• Domains: flavodoxin, NAD_binding_1, FAD_binding_1, NO_synthase
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway
• Gene Summary: 'Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]'