AMMECR1 (NM_001025580) Human Tagged ORF Clone

CAT#: RG209236

AMMECR1 (GFP-tagged) - Human Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1 (AMMECR1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AMMECR1
• Synonyms: AMMERC1; MFHIEN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209236 representing NM_001025580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGTTGCTGCGGGGTGAAGAAGCAGAAACTGTCCAGTTCGCCCCCCTCTGGCTCGGGTGGCG
  GTGGTGGCGCCTCCTCCTCCTCCCACTGCAGCGGAGAGAGCCAGTGCCGAGCTGGGGAGCTGGGACTAGG
  AGGCGCCGGTACGCGGCTCAACGGGCTGGGAGGTCTAACCGGAGGAGGTAGCGGCAGCGGCTGTACCCTC
  TCTCCCCCCCAGGGCTGCGGCGGCGGCGGCGGGGGGATCGCCCTGTCGCCACCTCCGAGCTGCGGAGTGG
  GGACCCTACTTTCTACCCCGGCCGCCGCCACCTCTTCCTCACCCTCCTCATCGTCCGCCGCCTCGTCCTC
  ATCGCCGGGCTCCCGGAAGATGGTGGTGTCAGCAGAGATGTGCTGCTTTTGCTTCGATGTGCTCTACTGT
  CACCTGTATGGATACCAGCAGCCCCGGACCCCCCGATTCACCAACGAGCCCTATGCCCTTAAAGATAGCC
  GTTTTCCCCCAATGACAAGGGATGAGCTGCCACGGCTTTTCTGCTCAGTGTCTCTGCTCACTAACTTTGA
  AGATGTCTGTGATTATTTGGACTGGGAGGTGGGTGTACATGGCATTAGAATAGAATTCATCAATGAAAAA
  GGATCAAAACGCACCGCCACCTACCTACCGGAGGTTGCAAAGGAGCAAGGATGGGACCATATACAGACCA
  TAGACTCCTTATTGAGGAAAGGAGGATACAAAGCTCCGATTACTAATGAATTCAGGAAAACCATAAAACT
  GACCAGGTATCGTAGTGAAAAGATGACCCTGAGCTATGCTGAATACCTTGCTCATCGCCAGCATCATCAT
  TTCCAAAATGGCATTGGGCATCCCCTTCCGCCATACAACCATTATTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209236 representing NM_001025580
  Red=Cloning site Green=Tags(s)
  
  MAAGCCGVKKQKLSSSPPSGSGGGGGASSSSHCSGESQCRAGELGLGGAGTRLNGLGGLTGGGSGSGCTL
  SPPQGCGGGGGGIALSPPPSCGVGTLLSTPAAATSSSPSSSSAASSSSPGSRKMVVSAEMCCFCFDVLYC
  HLYGYQQPRTPRFTNEPYALKDSRFPPMTRDELPRLFCSVSLLTNFEDVCDYLDWEVGVHGIRIEFINEK
  GSKRTATYLPEVAKEQGWDHIQTIDSLLRKGGYKAPITNEFRKTIKLTRYRSEKMTLSYAEYLAHRQHHH
  FQNGIGHPLPPYNHYS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025580
• ORF Size: 888 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025580.1, NP_001020751.1
• RefSeq Size: 5320 bp
• RefSeq ORF: 891 bp
• Locus ID: 9949
• Cytogenetics: Xq23
• Protein Families: Druggable Genome
• Gene Summary: The exact function of this gene is not known, however, submicroscopic deletion of the X chromosome including this gene, COL4A5, and FACL4 genes, result in a contiguous gene deletion syndrome, the AMME complex (Alport syndrome, mental retardation, midface hypoplasia, and elliptocytosis). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]