AMMECR1 (NM_001025580) Human Untagged Clone

CAT#: SC302419

AMMECR1 (untagged)-Human Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1 (AMMECR1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMMECR1
• Synonyms: AMMERC1; MFHIEN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001025580, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGGTTGCTGCGGGGTGAAGAAGCAGAAACTGTCCAGTTCGCCCCCCTCTGGCTCGGGTGGCG
  GTGGTGGCGCCTCCTCCTCCTCCCACTGCAGCGGAGAGAGCCAGTGCCGAGCTGGGGAGCTGGGACTAGG
  AGGCGCCGGTACGCGGCTCAACGGGCTGGGAGGTCTAACCGGAGGAGGTAGCGGCAGCGGCTGTACCCTC
  TCTCCCCCCCAGGGCTGCGGCGGCGGCGGCGGGGGGATCGCCCTGTCGCCACCTCCGAGCTGCGGAGTGG
  GGACCCTACTTTCTACCCCGGCCGCCGCCACCTCTTCCTCACCCTCCTCATCGTCCGCCGCCTCGTCCTC
  ATCGCCGGGCTCCCGGAAGATGGTGGTGTCAGCAGAGATGTGCTGCTTTTGCTTCGATGTGCTCTACTGT
  CACCTGTATGGATACCAGCAGCCCCGGACCCCCCGATTCACCAACGAGCCCTATGCCCTTAAAGATAGCC
  GTTTTCCCCCAATGACAAGGGATGAGCTGCCACGGCTTTTCTGCTCAGTGTCTCTGCTCACTAACTTTGA
  AGATGTCTGTGATTATTTGGACTGGGAGGTGGGTGTACATGGCATTAGAATAGAATTCATCAATGAAAAA
  GGATCAAAACGCACCGCCACCTACCTACCGGAGGTTGCAAAGGAGCAAGGATGGGACCATATACAGACCA
  TAGACTCCTTATTGAGGAAAGGAGGATACAAAGCTCCGATTACTAATGAATTCAGGAAAACCATAAAACT
  GACCAGGTATCGTAGTGAAAAGATGACCCTGAGCTATGCTGAATACCTTGCTCATCGCCAGCATCATCAT
  TTCCAAAATGGCATTGGGCATCCCCTTCCGCCATACAACCATTATTCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001025580
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025580.1, NP_001020751.1
• RefSeq Size: 5320 bp
• RefSeq ORF: 891 bp
• Locus ID: 9949
• Cytogenetics: Xq23
• Protein Families: Druggable Genome
• Gene Summary: The exact function of this gene is not known, however, submicroscopic deletion of the X chromosome including this gene, COL4A5, and FACL4 genes, result in a contiguous gene deletion syndrome, the AMME complex (Alport syndrome, mental retardation, midface hypoplasia, and elliptocytosis). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) is missing an in-frame coding exon compared to variant 1, resulting in a shorter isoform (2) lacking an internal protein segment compared to isoform 1.