Apc4 (ANAPC4) (NM_013367) Human Tagged ORF Clone

CAT#: RG209242

ANAPC4 (GFP-tagged) - Human anaphase promoting complex subunit 4 (ANAPC4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANAPC4
• Synonyms: APC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209242 representing NM_013367
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCGTTTTCCGACCTGTTTCCCATCCTTCCGGGTGGTGGGAGAGAAGCAGCTCCCGCAGGAGATTA
  TTTTCCTGGTCTGGTCGCCCAAGCGGGATCTCATTGCTTTGGCCAACACAGCTGGCGAGGTTTTACTTCA
  TCGACTGGCAAGTTTTCATCGAGTTTGGAGTTTTCCACCAAATGAAAATACAGGAAAGGAGGTGACGTGT
  CTGGCATGGAGACCAGATGGCAAACTTTTGGCCTTTGCTCTTGCTGATACCAAGAAAATTGTTTTGTGTG
  ATGTAGAAAAACCTGAGAGCTTACACTCTTTTTCTGTGGAGGCTCCAGTTTCCTGTATGCATTGGATGGA
  AGTGACAGTAGAAAGCAGTGTTCTCACATCATTTTATAATGCTGAGGATGAATCAAATCTTCTCTTACCT
  AAACTACCTACACTGCCAAAAAACTATAGCAACACCTCAAAAATATTTAGTGAAGAAAATTCTGATGAAA
  TTATTAAGCTCTTGGGAGACGTCAGGCTTAATATTCTCGTCCTTGGAGGAAGCTCTGGATTTATTGAGCT
  TTATGCTTATGGAATGTTTAAAATTGCTCGAGTCACAGGGATTGCTGGTACTTGTCTTGCATTATGTTTA
  TCAAGTGATTTGAAATCATTATCAGTGGTCACAGAAGTCTCTACCAATGGTGCTTCAGAAGTTTCATACT
  TTCAGCTTGAAACTAATCTGTTGTACTCTTTCTTACCTGAAGTAACTCGGATGGCCAGAAAGTTTACTCA
  TATTTCAGCTCTGTTACAGTATATAAATTTGTCACTAACATGTATGTGTGAAGCATGGGAAGAAATACTA
  ATGCAGATGGATTCTCGTCTCACCAAGTTTGTGCAGGAAAAGAACACAACCACATCAGTGCAAGATGAGT
  TCATGCACTTGCTATTATGGGGGAAAGCAAGTGCTGAACTTCAGACTCTCTTGATGAATCAGTTAACAGT
  AAAGGGCTTGAAAAAGCTTGGCCAGTCTATAGAGTCATCATACTCCAGTATACAAAAATTGGTCATAAGT
  CATTTACAGAGTGGCTCGGAGTCTTTATTATACCATTTGAGTGAATTGAAAGGAATGGCTTCATGGAAGC
  AAAAATATGAACCTCTTGGACTAGATGCTGCAGGAATCGAAGAAGCTATAACTGCTGTGGGTTCTTTTAT
  ACTCAAGGCAAATGAACTTCTTCAAGTTATAGATAGTAGTATGAAAAACTTCAAAGCATTTTTTCGGTGG
  CTTTATGTGGCAATGTTGAGAATGACAGAAGACCATGTGCTTCCCGAGCTGAATAAGATGACTCAGAAAG
  ATATCACATTTGTTGCTGAATTTCTTACTGAACATTTCAATGAGGCTCCAGACCTTTATAATCGAAAAGG
  AAAATACTTTAACGTTGAAAGAGTTGGTCAGTACTTGAAAGATGAAGATGATGATCTTGTGTCACCCCCT
  AACACAGAAGGAAACCAGTGGTATGACTTTCTTCAAAATAGCAGCCACCTTAAAGAAAGTCCTTTGCTGT
  TTCCTTATTATCCTCGAAAATCATTGCATTTTGTGAAAAGGCGGATGGAGAATATTATTGATCAGTGTTT
  GCAAAAGCCAGCAGATGTAATTGGAAAATCGATGAATCAAGCAATCTGTATTCCATTGTATAGAGATACC
  AGAAGTGAGGATTCTACACGTAGATTGTTCAAATTTCCTTTTCTGTGGAATAATAAAACTTCAAATCTAC
  ATTATCTTCTTTTTACTATTCTAGAAGATTCACTTTATAAAATGTGCATCTTAAGGAGACATACTGATAT
  TTCTCAATCTGTGAGTAATGGACTAATTGCTATTAAATTTGGGAGCTTTACATATGCCACAACAGAAAAA
  GTCAGAAGAAGCATCTACAGTTGTTTAGATGCACAGTTTTATGATGATGAAACTGTAACAGTAGTTCTTA
  AAGACACTGTAGGACGTGAAGGAAGAGATAGACTCTTGGTCCAGCTGCCTTTGTCTTTAGTATATAACAG
  TGAAGATTCTGCAGAATATCAGTTCACTGGGACTTATTCTACAAGGCTAGATGAACAGTGTAGTGCTATT
  CCCACCCGTACCATGCATTTCGAGAAGCACTGGAGATTACTGGAAAGTATGAAAGCACAGTATGTTGCTG
  GGAATGGTTTTCGAAAAGTGTCCTGTGTGTTAAGCTCAAATCTTCGTCATGTGAGAGTATTTGAAATGGA
  CATAGATGATGAATGGGAGCTCGATGGGTCTTCAGATGAAGAGGAGGAGGCCAGTAATAAGCCTGTAAAA
  ATAAAGGAAGAAGTGTTGTCGGAGTCAGAGGCAGAGAACCAACAAGCTGGTGCTGCCGCTTTAGCTCCAG
  AGATAGTCATTAAAGTGGAAAAACTTGACCCTGAGCTAGACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209242 representing NM_013367
  Red=Cloning site Green=Tags(s)
  
  MLRFPTCFPSFRVVGEKQLPQEIIFLVWSPKRDLIALANTAGEVLLHRLASFHRVWSFPPNENTGKEVTC
  LAWRPDGKLLAFALADTKKIVLCDVEKPESLHSFSVEAPVSCMHWMEVTVESSVLTSFYNAEDESNLLLP
  KLPTLPKNYSNTSKIFSEENSDEIIKLLGDVRLNILVLGGSSGFIELYAYGMFKIARVTGIAGTCLALCL
  SSDLKSLSVVTEVSTNGASEVSYFQLETNLLYSFLPEVTRMARKFTHISALLQYINLSLTCMCEAWEEIL
  MQMDSRLTKFVQEKNTTTSVQDEFMHLLLWGKASAELQTLLMNQLTVKGLKKLGQSIESSYSSIQKLVIS
  HLQSGSESLLYHLSELKGMASWKQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRW
  LYVAMLRMTEDHVLPELNKMTQKDITFVAEFLTEHFNEAPDLYNRKGKYFNVERVGQYLKDEDDDLVSPP
  NTEGNQWYDFLQNSSHLKESPLLFPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQAICIPLYRDT
  RSEDSTRRLFKFPFLWNNKTSNLHYLLFTILEDSLYKMCILRRHTDISQSVSNGLIAIKFGSFTYATTEK
  VRRSIYSCLDAQFYDDETVTVVLKDTVGREGRDRLLVQLPLSLVYNSEDSAEYQFTGTYSTRLDEQCSAI
  PTRTMHFEKHWRLLESMKAQYVAGNGFRKVSCVLSSNLRHVRVFEMDIDDEWELDGSSDEEEEASNKPVK
  IKEEVLSESEAENQQAGAAALAPEIVIKVEKLDPELDS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013367
• ORF Size: 2424 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013367.2, NP_037499.2
• RefSeq Size: 2701
• RefSeq ORF: 2427
• Locus ID: 29945
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]