BACH1 (NM_001186) Human Tagged ORF Clone

CAT#: RG209275

BACH1 (GFP-tagged) - Human BTB and CNC homology 1, basic leucine zipper transcription factor 1 (BACH1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BACH1
• Synonyms: BACH-1; BTBD24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209275 representing NM_001186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGAGTGAGAACTCGGTTTTTGCCTATGAATCTTCTGTGCATAGCACCAATGTTTTACTCAGCC
  TTAATGACCAGCGGAAGAAAGATGTGCTGTGCGATGTCACCATCTTTGTGGAGGGACAGCGGTTCCGCGC
  TCACCGGTCCGTGCTGGCGGCATGCAGCAGTTACTTCCACTCAAGAATCGTAGGCCAGGCTGATGGAGAG
  CTGAACATTACTCTTCCAGAAGAGGTGACAGTTAAAGGATTTGAACCTTTAATTCAGTTTGCCTACACTG
  CTAAACTGATTTTAAGTAAAGAGAATGTGGATGAAGTGTGCAAATGTGTGGAGTTTTTAAGTGTACATAA
  TATTGAGGAATCCTGCTTTCAGTTTCTGAAATTTAAGTTTTTGGACTCCACTGCAGACCAGCAAGAATGC
  CCAAGAAAAAAATGCTTTTCATCACACTGTCAGAAAACAGACCTTAAACTTTCACTTTTGGACCAGAGGG
  ATCTAGAAACTGATGAAGTGGAGGAATTTCTGGAAAATAAAAATGTTCAGACTCCTCAGTGTAAACTCCG
  CAGGTATCAAGGAAATGCAAAAGCCTCACCTCCTCTACAAGACAGTGCCAGTCAGACATATGAGTCCATG
  TGCTTAGAGAAGGATGCTGCTCTGGCCTTGCCTTCTTTATGCCCCAAATACAGAAAATTCCAAAAAGCAT
  TTGGAACTGACAGAGTCCGTACTGGGGAATCTAGTGTCAAAGACATTCATGCTTCTGTTCAGCCAAATGA
  AAGGTCTGAAAATGAATGCCTGGGAGGAGTCCCGGAGTGTAGAGATTTGCAGGTGATGTTAAAATGTGAC
  GAAAGTAAATTAGCAATGGAACCTGAAGAAACGAAGAAAGATCCTGCTTCTCAGTGCCCAACTGAAAAAT
  CAGAAGTGACTCCTTTCCCCCACAATTCTTCCATAGACCCTCATGGACTTTATTCTTTGTCTCTTTTACA
  CACATATGACCAATATGGTGACTTGAATTTTGCTGGTATGCAAAACACAACAGTGTTAACAGAAAAGCCT
  TTGTCAGGTACAGACGTCCAAGAAAAAACATTTGGTGAAAGTCAGGATTTACCTTTGAAATCCGACTTGG
  GCACCAGGGAAGATAGTAGTGTTGCATCTAGTGATAGGAGTAGTGTGGAGCGAGAAGTGGCAGAACACCT
  AGCAAAAGGCTTCTGGAGTGACATTTGCAGCACGGACACTCCTTGCCAAATGCAGTTATCACCTGCTGTG
  GCCAAAGATGGCTCAGAACAGATCTCACAGAAACGGTCTGAGTGTCCGTGGTTAGGTATCAGGATTAGTG
  AGAGCCCAGAACCAGGTCAAAGGACTTTCACAACATTAAGTTCTGTCAACTGCCCTTTTATAAGTACTCT
  GAGTACTGAAGGCTGTTCAAGCAATTTGGAAATTGGAAACGATGATTATGTTTCAGAACCCCAGCAAGAA
  CCTTGCCCATATGCTTGTGTCATTAGCTTGGGAGACGACTCTGAGACGGACACCGAAGGAGACAGTGAAT
  CCTGTTCAGCCAGAGAACAAGAATGTGAGGTAAAACTGCCATTCAATGCACAACGGATAATTTCACTGTC
  TCGAAATGATTTTCAGTCCTTGTTGAAAATGCACAAGCTTACTCCAGAACAGCTGGATTGTATCCATGAT
  ATTCGAAGAAGAAGTAAAAACAGAATTGCTGCACAGCGCTGTCGCAAGAGAAAACTTGACTGTATACAGA
  ATCTTGAATCAGAAATTGAGAAGCTGCAAAGTGAAAAGGAGAGCTTGTTGAAGGAAAGAGATCACATTTT
  GTCAACTCTGGGTGAGACAAAGCAGAACCTAACTGGACTTTGCCAGAAAGTTTGTAAAGAAGCAGCTCTG
  AGTCAAGAACAAATACAGATACTCGCCAAGTACTCAGCTGCAGATTGCCCACTTTCATTTTTAATTTCTG
  AAAAAGATAAAAGTACTCCTGATGGTGAACTGGCGTTACCATCAATTTTCAGTTTATCTGACCGGCCTCC
  AGCAGTGCTGCCTCCCTGTGCCAGAGGAAACAGTGAGCCTGGCTACGCGCGAGGGCAGGAGTCCCAGCAG
  ATGTCCACAGCCACCTCTGAGCAAGCTGGGCCTGCGGAACAGTGTCGTCAGAGTGGTGGGATCTCAGATT
  TCTGTCAGCAGATGACTGATAAATGTACTACTGATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209275 representing NM_001186
  Red=Cloning site Green=Tags(s)
  
  MSLSENSVFAYESSVHSTNVLLSLNDQRKKDVLCDVTIFVEGQRFRAHRSVLAACSSYFHSRIVGQADGE
  LNITLPEEVTVKGFEPLIQFAYTAKLILSKENVDEVCKCVEFLSVHNIEESCFQFLKFKFLDSTADQQEC
  PRKKCFSSHCQKTDLKLSLLDQRDLETDEVEEFLENKNVQTPQCKLRRYQGNAKASPPLQDSASQTYESM
  CLEKDAALALPSLCPKYRKFQKAFGTDRVRTGESSVKDIHASVQPNERSENECLGGVPECRDLQVMLKCD
  ESKLAMEPEETKKDPASQCPTEKSEVTPFPHNSSIDPHGLYSLSLLHTYDQYGDLNFAGMQNTTVLTEKP
  LSGTDVQEKTFGESQDLPLKSDLGTREDSSVASSDRSSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAV
  AKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQE
  PCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHD
  IRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAAL
  SQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQ
  MSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001186
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001186.2, NP_001177.1
• RefSeq Size: 5642 bp
• RefSeq ORF: 2211 bp
• Locus ID: 571
• Cytogenetics: 21q21.3
• Domains: BTB, BRLZ
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, May 2009]'