PARD6B (NM_032521) Human Tagged ORF Clone

CAT#: RG209290

PARD6B (GFP-tagged) - Human par-6 partitioning defective 6 homolog beta (C. elegans) (PARD6B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PARD6B
• Synonyms: PAR6B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209290 representing NM_032521
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGCAGCCACCGGCACGGGGCGGGCAGCGGCTGCCTGGGCACTATGGAGGTGAAGAGCAAGTTTG
  GAGCTGAATTTCGTCGGTTTTCGCTGGAAAGATCAAAACCTGGAAAATTTGAGGAGTTTTATGGATTACT
  ACAACATGTTCATAAGATCCCCAATGTTGACGTTTTGGTAGGCTATGCAGACATCCATGGAGACTTACTA
  CCTATAAATAATGATGATAATTATCACAAAGCTGTTTCAACGGCCAATCCACTGCTTAGGATATTTATAC
  AAAAGAAGGAAGAAGCAGACTACAGTGCCTTTGGTACAGACACGCTAATAAAGAAGAAGAATGTTTTAAC
  CAACGTATTGCGTCCTGACAACCATAGAAAAAAGCCACATATAGTCATTAGTATGCCCCAAGACTTTAGA
  CCTGTGTCTTCTATTATAGACGTGGATATTCTCCCAGAAACGCATCGTAGGGTACGTCTTTACAAATACG
  GCACGGAGAAACCCCTAGGATTCTACATCCGGGATGGCTCCAGTGTCAGGGTAACACCACATGGCTTAGA
  AAAGGTTCCAGGGATCTTTATATCCAGGCTTGTCCCAGGAGGTCTGGCTCAAAGTACAGGACTATTAGCT
  GTTAATGATGAAGTTTTAGAAGTTAATGGCATAGAAGTTTCAGGGAAGAGCCTTGATCAAGTAACAGACA
  TGATGATTGCAAATAGCCGTAACCTCATCATAACAGTGAGACCGGCAAACCAGAGGAATAATGTTGTGAG
  GAACAGTCGGACTTCTGGCAGTTCCGGTCAGTCTACTGATAACAGCCTTCTTGGCTACCCACAGCAGATT
  GAACCAAGCTTTGAGCCAGAGGATGAAGACAGCGAAGAAGATGACATTATCATTGAAGACAATGGAGTGC
  CACAGCAGATTCCAAAAGCTGTTCCTAATACTGAGAGCCTGGAGTCATTAACACAGATAGAGTTAAGCTT
  TGAGTCTGGACAGAATGGCTTTATTCCCTCTAATGAAGTGAGCTTAGCAGCCATAGCAAGCAGCTCAAAC
  ACGGAATTTGAAACACATGCTCCAGATCAAAAACTCTTAGAAGAAGATGGAACAATCATAACATTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209290 representing NM_032521
  Red=Cloning site Green=Tags(s)
  
  MNRSHRHGAGSGCLGTMEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLL
  PINNDDNYHKAVSTANPLLRIFIQKKEEADYSAFGTDTLIKKKNVLTNVLRPDNHRKKPHIVISMPQDFR
  PVSSIIDVDILPETHRRVRLYKYGTEKPLGFYIRDGSSVRVTPHGLEKVPGIFISRLVPGGLAQSTGLLA
  VNDEVLEVNGIEVSGKSLDQVTDMMIANSRNLIITVRPANQRNNVVRNSRTSGSSGQSTDNSLLGYPQQI
  EPSFEPEDEDSEEDDIIIEDNGVPQQIPKAVPNTESLESLTQIELSFESGQNGFIPSNEVSLAAIASSSN
  TEFETHAPDQKLLEEDGTIITL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032521
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_032521.1, NP_115910.1
• RefSeq Size: 3655
• RefSeq ORF: 1119
• Locus ID: 84612
• Protein Pathways: Endocytosis, Tight junction
• Gene Summary: This gene is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. This cytoplasmic protein is involved in asymmetrical cell division and cell polarization processes as a member of a multi-protein complex. [provided by RefSeq, Jul 2008]