Pyrophosphatase 1 (PPA1) (NM_021129) Human Tagged ORF Clone
CAT#: RG209393
- TrueORF®
PPA1 (GFP-tagged) - Human pyrophosphatase (inorganic) 1 (PPA1)
"NM_021129" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PPA1 |
Synonyms | HEL-S-66p; IOPPP; PP; PP1; SID6-8061 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209393 representing NM_021129
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCTTCAGCACCGAGGAGCGCGCCGCGCCCTTCTCCCTGGAGTACCGAGTCTTCCTCAAAAATG AGAAAGGACAATATATATCTCCATTTCATGATATTCCAATTTATGCAGATAAGGATGTGTTTCACATGGT AGTTGAAGTACCACGCTGGTCTAATGCAAAAATGGAGATTGCTACAAAGGACCCTTTAAACCCTATTAAA CAAGATGTGAAAAAAGGAAAACTTCGCTATGTTGCGAATTTGTTCCCGTATAAAGGATATATCTGGAACT ATGGTGCCATCCCTCAGACTTGGGAAGACCCAGGGCACAATGATAAACATACTGGCTGTTGTGGTGACAA TGACCCAATTGATGTGTGTGAAATTGGAAGCAAGGTATGTGCAAGAGGTGAAATAATTGGCGTGAAAGTT CTAGGCATATTGGCTATGATTGACGAAGGGGAAACCGACTGGAAAGTCATTGCCATTAATGTGGATGATC CTGATGCAGCCAATTATAATGATATCAATGATGTCAAACGGCTGAAACCTGGCTACTTAGAAGCTACTGT GGACTGGTTTAGAAGGTATAAGGTTCCTGATGGAAAACCAGAAAATGAGTTTGCGTTTAATGCAGAATTT AAAGATAAGGACTTTGCCATTGATATTATTAAAAGCACTCATGACCATTGGAAAGCATTAGTGACTAAGA AAACGAATGGAAAAGGAATCAGTTGCATGAATACAACTTTGTCTGAGAGCCCCTTCAAGTGTGATCCTGA TGCTGCCAGAGCCATTGTGGATGCTTTACCACCACCCTGTGAATCTGCCTGCACAGTACCAACAGACGTG GATAAGTGGTTCCATCACCAGAAAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209393 representing NM_021129
Red=Cloning site Green=Tags(s) MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIK QDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKV LGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEF KDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDV DKWFHHQKN TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021129 |
ORF Size | 867 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021129.3, NP_066952.1 |
RefSeq Size | 1316 bp |
RefSeq ORF | 870 bp |
Locus ID | 5464 |
Cytogenetics | 10q22.1 |
Domains | Pyrophosphatase |
Protein Families | ES Cell Differentiation/IPS |
Protein Pathways | Oxidative phosphorylation |
Gene Summary | 'The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC112976 | PPA1 (untagged)-Human pyrophosphatase (inorganic) 1 (PPA1) |
USD 660.00 |
|
RC209393 | PPA1 (Myc-DDK-tagged)-Human pyrophosphatase (inorganic) 1 (PPA1) |
USD 420.00 |
|
RC209393L1 | Lenti ORF clone of Human pyrophosphatase (inorganic) 1 (PPA1), Myc-DDK-tagged |
USD 768.00 |
|
RC209393L2 | Lenti ORF clone of Human pyrophosphatase (inorganic) 1 (PPA1), mGFP tagged |
USD 620.00 |
|
RC209393L3 | Lenti ORF clone of Human pyrophosphatase (inorganic) 1 (PPA1), Myc-DDK-tagged |
USD 620.00 |
|
RC209393L4 | Lenti ORF clone of Human pyrophosphatase (inorganic) 1 (PPA1), mGFP tagged |
USD 620.00 |
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