Pyrophosphatase 1 (PPA1) (NM_021129) Human Tagged ORF Clone

CAT#: RC209393

PPA1 (Myc-DDK-tagged)-Human pyrophosphatase (inorganic) 1 (PPA1)

Product Images

Western blot validation of overexpression lysate (Cat# LY412068) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209393 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PPA1 protein (Cat# TP309393). The protein was produced from HEK293T cells transfected with PPA1 cDNA clone (Cat# RC209393) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPA1
• Synonyms: HEL-S-66p; IOPPP; PP; PP1; SID6-8061
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209393 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCTTCAGCACCGAGGAGCGCGCCGCGCCCTTCTCCCTGGAGTACCGAGTCTTCCTCAAAAATG
  AGAAAGGACAATATATATCTCCATTTCATGATATTCCAATTTATGCAGATAAGGATGTGTTTCACATGGT
  AGTTGAAGTACCACGCTGGTCTAATGCAAAAATGGAGATTGCTACAAAGGACCCTTTAAACCCTATTAAA
  CAAGATGTGAAAAAAGGAAAACTTCGCTATGTTGCGAATTTGTTCCCGTATAAAGGATATATCTGGAACT
  ATGGTGCCATCCCTCAGACTTGGGAAGACCCAGGGCACAATGATAAACATACTGGCTGTTGTGGTGACAA
  TGACCCAATTGATGTGTGTGAAATTGGAAGCAAGGTATGTGCAAGAGGTGAAATAATTGGCGTGAAAGTT
  CTAGGCATATTGGCTATGATTGACGAAGGGGAAACCGACTGGAAAGTCATTGCCATTAATGTGGATGATC
  CTGATGCAGCCAATTATAATGATATCAATGATGTCAAACGGCTGAAACCTGGCTACTTAGAAGCTACTGT
  GGACTGGTTTAGAAGGTATAAGGTTCCTGATGGAAAACCAGAAAATGAGTTTGCGTTTAATGCAGAATTT
  AAAGATAAGGACTTTGCCATTGATATTATTAAAAGCACTCATGACCATTGGAAAGCATTAGTGACTAAGA
  AAACGAATGGAAAAGGAATCAGTTGCATGAATACAACTTTGTCTGAGAGCCCCTTCAAGTGTGATCCTGA
  TGCTGCCAGAGCCATTGTGGATGCTTTACCACCACCCTGTGAATCTGCCTGCACAGTACCAACAGACGTG
  GATAAGTGGTTCCATCACCAGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209393 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIK
  QDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKV
  LGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEF
  KDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDV
  DKWFHHQKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021129
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021129.1, NM_021129.2, NM_021129.3, NP_066952.1
• RefSeq Size: 1316 bp
• RefSeq ORF: 870 bp
• Locus ID: 5464
• Cytogenetics: 10q22.1
• Domains: Pyrophosphatase
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Oxidative phosphorylation
• MW: 32.7 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008]'