TANK (NM_004180) Human Tagged ORF Clone

CAT#: RG209759

TANK (GFP-tagged) - Human TRAF family member-associated NFKB activator (TANK), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TANK
• Synonyms: I-TRAF; ITRAF; TRAF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209759 representing NM_004180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAAAACATTGGCGAGCAACTCAATAAAGCGTATGAAGCCTTCCGGCAGGCATGCATGGATAGAG
  ATTCTGCAGTAAAAGAATTACAGCAAAAGACTGAGAACTATGAGCAGAGAATACGTGAACAACAGGAACA
  GCTGTCACTTCAACAGACTATTATTGACAAGCTAAAATCTCAGTTACTTCTTGTGAATTCCACTCAAGAT
  AACAATTATGGCTGTGTTCCTCTGCTTGAAGACAGTGAAACAAGAAAGAATAATTTGACTCTTGATCAGC
  CACAAGATAAAGTGATTTCAGGAATAGCAAGAGAAAAACTACCAAAGGTAAGAAGACAAGAGGTTTCTTC
  TCCTAGAAAAGAAACTTCAGCAAGGAGTCTTGGCAGTCCTTTGCTCCATGAAAGGGGTAATATAGAGAAG
  ACTTTCTGGGATCTGAAAGAAGAATTTCATAAAATATGCATGCTAGCAAAAGCACAGAAAGACCACTTAA
  GCAAACTTAATATACCAGACACTGCAACTGAAACACAGTGCTCTGTGCCTATACAGTGTACGGATAAAAC
  AGATAAACAAGAAGCGCTGTTTAAGCCTCAGGCTAAAGATGATATAAATAGAGGTGCACCATCCATCACA
  TCTGTCACACCAAGAGGACTGTGCAGAGATGAGGAAGACACCTCTTTTGAATCACTTTCTAAATTCAATG
  TCAAGTTTCCACCTATGGACAATGACTCAACTTTCTTACATAGCACTCCAGAGAGACCCGGCATCCTTAG
  TCCTGCCACGTCTGAGGCAGTGTGCCAAGAGAAATTTAATATGGAGTTCAGAGACAACCCAGGGAACTTT
  GTTAAAACAGAAGAAACTTTATTTGAAATTCAGGGAATTGACCCCATAGCTTCAGCTATACAAAACCTTA
  AAACAACTGACAAAACAAAGCCCTCAAATCTCGTAAACACTTGTATCAGGACAACTCTGGATAGAGCTGC
  GTGTTTGCCACCTGGAGACCATAATGCATTATATGTAAATAGCTTCCCACTTCTGGACCCATCTGATGCA
  CCTTTTCCCTCACTCGATTCCCCGGGAAAAGCAATCCGAGGACCACAGCAGCCCATTTGGAAGCCCTTTC
  CTAATCAAGACAGTGACTCGGTGGTACTAAGTGGCACAGACTCAGAACTGCATATACCTCGAGTATGTGA
  ATTCTGTCAAGCAGTTTTCCCACCATCCATTACATCCAGGGGGGATTTCCTTCGGCATCTTAATTCACAC
  TTCAATGGAGAGACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209759 representing NM_004180
  Red=Cloning site Green=Tags(s)
  
  MDKNIGEQLNKAYEAFRQACMDRDSAVKELQQKTENYEQRIREQQEQLSLQQTIIDKLKSQLLLVNSTQD
  NNYGCVPLLEDSETRKNNLTLDQPQDKVISGIAREKLPKVRRQEVSSPRKETSARSLGSPLLHERGNIEK
  TFWDLKEEFHKICMLAKAQKDHLSKLNIPDTATETQCSVPIQCTDKTDKQEALFKPQAKDDINRGAPSIT
  SVTPRGLCRDEEDTSFESLSKFNVKFPPMDNDSTFLHSTPERPGILSPATSEAVCQEKFNMEFRDNPGNF
  VKTEETLFEIQGIDPIASAIQNLKTTDKTKPSNLVNTCIRTTLDRAACLPPGDHNALYVNSFPLLDPSDA
  PFPSLDSPGKAIRGPQQPIWKPFPNQDSDSVVLSGTDSELHIPRVCEFCQAVFPPSITSRGDFLRHLNSH
  FNGET
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004180
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004180.2, NP_004171.2
• RefSeq Size: 2089
• RefSeq ORF: 1278
• Locus ID: 10010
• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]