PAPP A (PAPPA) (NM_002581) Human Tagged ORF Clone

CAT#: RG209767

PAPPA (GFP-tagged) - Human pregnancy-associated plasma protein A, pappalysin 1 (PAPPA)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PAPPA
• Synonyms: ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209767 representing NM_002581
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTCTGGAGTTGGGTGCTGCACCTGGGGCTGCTGAGCGCCGCGCTGGGCTGCGGGCTGGCCGAGC
  GTCCCCGCCGGGCCCGGAGAGACCCGCGGGCCGGCCGACCCCCGCGCCCCGCCGCCGGCCCGGCCACCTG
  CGCCACCCGGGCGGCCCGCGGCCGCCGCGCCTCGCCGCCGCCGCCGCCGCCGCCGGGCGGTGCCTGGGAA
  GCCGTGCGCGTCCCCCGGCGGCGGCAGCAGCGGGAGGCGAGGGGCGCCACCGAGGAGCCGAGCCCGCCGA
  GCCGGGCGCTCTATTTCAGCGGGCGAGGCGAGCAGCTGCGCCTCCGGGCCGACCTCGAGCTGCCCCGGGA
  CGCGTTCACGCTGCAAGTGTGGCTGCGAGCGGAGGGGGGCCAGAGGTCTCCGGCAGTGATCACAGGGCTG
  TATGACAAATGTTCTTATATCTCACGTGACCGAGGATGGGTCGTGGGCATTCACACCATCAGTGACCAAG
  ACAACAAAGACCCACGCTACTTTTTCTCCTTGAAGACAGACCGAGCCCGGCAAGTGACCACCATCAATGC
  CCACCGCAGCTACCTCCCAGGCCAGTGGGTATACCTAGCTGCCACCTATGATGGGCAGTTCATGAAGCTC
  TATGTGAATGGTGCCCAGGTGGCCACCTCTGGGGAACAAGTGGGTGGCATATTCAGCCCACTGACCCAGA
  AGTGCAAAGTGCTCATGTTAGGGGGCAGTGCCCTGAATCACAACTACCGGGGCTACATCGAGCACTTCAG
  TCTGTGGAAGGTGGCCAGGACTCAGCGGGAGATACTGTCTGACATGGAAACCCATGGCGCCCACACTGCT
  CTACCTCAGCTCCTCCTCCAGGAGAACTGGGACAATGTGAAGCATGCCTGGTCCCCCATGAAGGATGGCA
  GCAGCCCCAAAGTGGAATTCAGCAATGCCCACGGCTTTCTGCTGGACACGAGTCTGGAGCCTCCTCTGTG
  TGGACAGACATTGTGTGACAACACAGAGGTCATTGCCAGCTACAATCAGCTCTCAAGTTTCCGCCAGCCC
  AAGGTGGTGCGCTACCGCGTGGTCAACCTCTATGAAGATGATCATAAGAACCCGACGGTGACGCGCGAGC
  AGGTGGACTTCCAGCACCATCAGCTGGCTGAGGCCTTCAAGCAATACAACATCTCCTGGGAGCTGGACGT
  GCTGGAGGTGAGCAACTCCTCTCTTCGCCGCCGCCTCATCCTGGCCAACTGTGACATCAGCAAGATTGGG
  GATGAGAACTGTGACCCCGAGTGCAACCACACGCTGACGGGCCACGACGGCGGGGATTGCCGCCACCTGC
  GCCACCCTGCCTTCGTGAAGAAGCAGCACAACGGGGTGTGTGACATGGACTGCAACTATGAACGGTTCAA
  CTTTGATGGTGGAGAGTGCTGTGACCCTGAAATCACCAATGTCACTCAGACTTGCTTTGACCCCGACTCT
  CCACACAGAGCCTACTTGGATGTTAATGAGCTGAAGAACATTCTTAAATTGGATGGATCAACACATCTCA
  ATATTTTCTTTGCAAAATCCTCAGAGGAGGAGTTGGCAGGAGTAGCAACTTGGCCATGGGACAAGGAGGC
  CCTGATGCACTTAGGTGGCATTGTCTTGAACCCATCTTTCTATGGCATGCCTGGGCACACCCACACCATG
  ATCCATGAGATTGGTCACAGCCTGGGCCTCTATCACGTCTTCCGAGGCATCTCAGAAATCCAGTCCTGCA
  GTGACCCCTGCATGGAGACAGAGCCCTCCTTCGAGACTGGAGACCTCTGCAATGATACCAACCCAGCCCC
  TAAACACAAGTCCTGTGGTGACCCAGGGCCAGGAAATGACACCTGTGGCTTTCATAGCTTCTTCAACACT
  CCTTACAACAACTTCATGAGCTATGCAGATGACGACTGTACGGACTCCTTCACGCCCAATCAAGTCGCCA
  GAATGCACTGTTACCTGGACCTGGTCTACCAGGGCTGGCAGCCCTCCAGGAAACCAGCGCCTGTTGCCCT
  CGCCCCCCAAGTTCTGGGCCACACAACGGACTCTGTGACACTGGAGTGGTTCCCACCTATAGATGGCCAT
  TTCTTTGAAAGAGAATTGGGATCAGCATGTCATCTTTGCCTGGAAGGGAGAATCCTGGTGCAGTATGCTT
  CCAACGCTTCCTCCCCAATGCCCTGCAGCCCATCAGGACACTGGAGCCCTCGTGAAGCAGAAGGTCATCC
  TGATGTTGAACAGCCCTGTAAGTCCAGTGTCCGCACCTGGAGCCCAAATTCAGCTGTCAACCCACACACG
  GTTCCTCCAGCCTGCCCTGAGCCTCAAGGCTGCTACCTCGAGCTGGAGTTCCTCTACCCCTTGGTCCCTG
  AGTCTCTGACCATTTGGGTGACCTTTGTCTCCACTGACTGGGACTCTAGTGGAGCTGTCAATGACATCAA
  ACTGTTGGCTGTCAGTGGGAAGAACATCTCCCTGGGTCCTCAGAATGTCTTCTGTGATGTCCCACTGACC
  ATCAGACTCTGGGACGTGGGCGAGGAGGTGTATGGCATCCAAATCTACACGCTGGATGAGCACCTGGAGA
  TCGATGCTGCCATGTTGACCTCCACTGCAGACACCCCACTCTGTCTACAGTGTAAGCCCCTGAAGTATAA
  GGTGGTCCGGGACCCTCCTCTCCAGATGGATGTGGCCTCCATCCTACATCTCAATAGGAAATTCGTAGAC
  ATGGATCTAAATCTTGGCAGTGTGTACCAGTATTGGGTCATAACTATTTCAGGAACTGAAGAGAGTGAGC
  CATCACCTGCTGTCACATACATCCATGGAAGTGGGTACTGTGGCGATGGCATTATACAAAAAGACCAAGG
  TGAACAATGCGACGACATGAATAAGATCAATGGTGATGGCTGCTCCCTTTTCTGCCGACAAGAAGTCTCC
  TTCAATTGTATTGATGAACCCAGCCGGTGCTATTTCCATGATGGTGATGGGGTATGTGAGGAGTTTGAAC
  AAAAAACCAGCATTAAGGACTGTGGTGTCTACACGCCCCAGGGATTCCTGGATCAGTGGGCATCCAATGC
  TTCAGTATCTCATCAAGACCAGCAATGCCCAGGCTGGGTCATCATCGGACAGCCAGCAGCATCCCAGGTG
  TGTCGAACCAAGGTGATAGATCTCAGTGAAGGCATTTCCCAGCATGCCTGGTACCCTTGCACCATCAGCT
  ACCCATATTCCCAGCTGGCTCAGACCACTTTTTGGCTCCGGGCGTATTTTTCTCAACCAATGGTTGCCGC
  AGCTGTCATTGTCCACCTGGTGACGGATGGGACATATTATGGGGACCAAAAGCAGGAGACCATCAGCGTG
  CAGCTGCTTGATACCAAAGATCAGAGCCACGATCTAGGCCTCCATGTCCTGAGCTGCAGGAACAATCCCC
  TGATTATCCCTGTGGTCCATGACCTCAGCCAGCCCTTCTACCACAGCCAGGCGGTACGTGTGAGCTTCAG
  TTCGCCCCTGGTCGCCATCTCGGGGGTGGCCCTCCGTTCCTTCGACAACTTTGACCCCGTCACCCTGAGC
  AGCTGCCAGAGAGGGGAGACCTACAGCCCTGCCGAGCAGAGCTGCGTGCACTTCGCATGTGAGAAAACTG
  ACTGTCCAGAGCTGGCTGTGGAGAATGCTTATCTCAATTGCTCCAGCAGCGACCGCTACCACGGTGCCCA
  GTGTACTGTGAGCTGCCGGACAGGCTACGTGCTCCAGATACGGCGGGATGATGAGCTGATCAAGAGCCAG
  ACGGGACCCAGCGTCACAGTGACCTGTACAGAGGGCAAGTGGAATAAGCAGGTGGCCTGTGAGCCAGTCG
  ACTGCAGCATCCCAGATCACCATCAAGTCTATGCTGCCTCCTTCTCCTGCCCTGAGGGCACCACCTTTGG
  CAGTCAATGTTCCTTCCAGTGCCGTCACCCTGCACAATTGAAAGGCAACAACAGCCTCCTGACCTGCATG
  GAGGATGGGCTGTGGTCCTTCCCAGAGGCCCTGTGTGAGCTCATGTGCCTCGCTCCACCCCCTGTGCCCA
  ATGCAGACCTCCAGACCGCCCGGTGCCGAGAGAATAAGCACAAGGTGGGCTCCTTCTGCAAATACAAATG
  CAAGCCTGGATACCATGTGCCTGGATCCTCTCGGAAGTCAAAGAAACGGGCCTTCAAGACTCAGTGTACC
  CAGGATGGCAGCTGGCAGGAGGGAGCTTGTGTTCCTGTGACCTGTGACCCACCTCCACCAAAATTCCATG
  GGCTCTACCAGTGTACTAATGGCTTCCAGTTCAACAGTGAGTGTAGGATCAAGTGTGAAGACAGTGATGC
  CTCCCAGGGACTTGGGAGCAATGTCATTCATTGCCGGAAAGATGGCACCTGGAACGGCTCCTTCCATGTC
  TGCCAGGAGATGCAAGGCCAGTGCTCGGTTCCAAACGAGCTCAACAGCAACCTCAAACTGCAGTGCCCTG
  ATGGCTATGCCATAGGGTCGGAGTGTGCCACCTCGTGCCTGGACCACAACAGCGAGTCCATCATCCTGCC
  AATGAACGTGACCGTGCGTGACATCCCCCACTGGCTGAACCCCACACGGGTAGAGAGAGTTGTCTGCACT
  GCTGGTCTCAAGTGGTATCCTCACCCTGCTCTGATTCACTGTGTCAAAGGCTGTGAGCCCTTCATGGGAG
  ACAATTATTGTGATGCCATCAACAACCGAGCCTTTTGCAACTATGACGGTGGGGATTGCTGCACCTCCAC
  AGTGAAGACCAAAAAGGTCACCCCATTCCCTATGTCCTGTGACCTACAAGGTGACTGTGCTTGTCGGGAC
  CCCCAGGCCCAAGAACACAGCCGGAAAGACCTCCAGGGATACAGCCATGGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209767 representing NM_002581
  Red=Cloning site Green=Tags(s)
  
  MRLWSWVLHLGLLSAALGCGLAERPRRARRDPRAGRPPRPAAGPATCATRAARGRRASPPPPPPPGGAWE
  AVRVPRRRQQREARGATEEPSPPSRALYFSGRGEQLRLRADLELPRDAFTLQVWLRAEGGQRSPAVITGL
  YDKCSYISRDRGWVVGIHTISDQDNKDPRYFFSLKTDRARQVTTINAHRSYLPGQWVYLAATYDGQFMKL
  YVNGAQVATSGEQVGGIFSPLTQKCKVLMLGGSALNHNYRGYIEHFSLWKVARTQREILSDMETHGAHTA
  LPQLLLQENWDNVKHAWSPMKDGSSPKVEFSNAHGFLLDTSLEPPLCGQTLCDNTEVIASYNQLSSFRQP
  KVVRYRVVNLYEDDHKNPTVTREQVDFQHHQLAEAFKQYNISWELDVLEVSNSSLRRRLILANCDISKIG
  DENCDPECNHTLTGHDGGDCRHLRHPAFVKKQHNGVCDMDCNYERFNFDGGECCDPEITNVTQTCFDPDS
  PHRAYLDVNELKNILKLDGSTHLNIFFAKSSEEELAGVATWPWDKEALMHLGGIVLNPSFYGMPGHTHTM
  IHEIGHSLGLYHVFRGISEIQSCSDPCMETEPSFETGDLCNDTNPAPKHKSCGDPGPGNDTCGFHSFFNT
  PYNNFMSYADDDCTDSFTPNQVARMHCYLDLVYQGWQPSRKPAPVALAPQVLGHTTDSVTLEWFPPIDGH
  FFERELGSACHLCLEGRILVQYASNASSPMPCSPSGHWSPREAEGHPDVEQPCKSSVRTWSPNSAVNPHT
  VPPACPEPQGCYLELEFLYPLVPESLTIWVTFVSTDWDSSGAVNDIKLLAVSGKNISLGPQNVFCDVPLT
  IRLWDVGEEVYGIQIYTLDEHLEIDAAMLTSTADTPLCLQCKPLKYKVVRDPPLQMDVASILHLNRKFVD
  MDLNLGSVYQYWVITISGTEESEPSPAVTYIHGSGYCGDGIIQKDQGEQCDDMNKINGDGCSLFCRQEVS
  FNCIDEPSRCYFHDGDGVCEEFEQKTSIKDCGVYTPQGFLDQWASNASVSHQDQQCPGWVIIGQPAASQV
  CRTKVIDLSEGISQHAWYPCTISYPYSQLAQTTFWLRAYFSQPMVAAAVIVHLVTDGTYYGDQKQETISV
  QLLDTKDQSHDLGLHVLSCRNNPLIIPVVHDLSQPFYHSQAVRVSFSSPLVAISGVALRSFDNFDPVTLS
  SCQRGETYSPAEQSCVHFACEKTDCPELAVENAYLNCSSSDRYHGAQCTVSCRTGYVLQIRRDDELIKSQ
  TGPSVTVTCTEGKWNKQVACEPVDCSIPDHHQVYAASFSCPEGTTFGSQCSFQCRHPAQLKGNNSLLTCM
  EDGLWSFPEALCELMCLAPPPVPNADLQTARCRENKHKVGSFCKYKCKPGYHVPGSSRKSKKRAFKTQCT
  QDGSWQEGACVPVTCDPPPPKFHGLYQCTNGFQFNSECRIKCEDSDASQGLGSNVIHCRKDGTWNGSFHV
  CQEMQGQCSVPNELNSNLKLQCPDGYAIGSECATSCLDHNSESIILPMNVTVRDIPHWLNPTRVERVVCT
  AGLKWYPHPALIHCVKGCEPFMGDNYCDAINNRAFCNYDGGDCCTSTVKTKKVTPFPMSCDLQGDCACRD
  PQAQEHSRKDLQGYSHG
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002581
• ORF Size: 4881 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002581.3, NP_002572.2
• RefSeq Size: 11025
• RefSeq ORF: 4884
• Locus ID: 5069
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Gene Summary: This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017]