PSMD14 (NM_005805) Human Tagged ORF Clone

CAT#: RG209829

PSMD14 (GFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 (PSMD14)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PSMD14
• Synonyms: PAD1; POH1; RPN11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209829 representing NM_005805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGACTTCTTAGACTTGGAGGAGGTATGCCTGGACTGGGCCAGGGGCCACCTACAGATGCTCCTG
  CAGTGGACACAGCAGAACAAGTCTATATCTCTTCCCTGGCACTGTTAAAAATGTTAAAACATGGCCGTGC
  TGGAGTTCCAATGGAAGTTATGGGTTTGATGCTTGGAGAATTTGTTGATGATTATACCGTCAGAGTGATT
  GATGTGTTTGCTATGCCACAGTCAGGAACAGGTGTCAGTGTGGAGGCAGTTGATCCAGTGTTCCAAGCTA
  AAATGTTGGATATGTTGAAGCAGACAGGAAGGCCGGAGATGGTTGTTGGTTGGTATCACAGTCACCCTGG
  CTTTGGTTGTTGGCTTTCTGGTGTGGATATCAACACTCAGCAGAGCTTTGAAGCCTTGTCGGAGAGAGCT
  GTGGCAGTGGTTGTGGATCCCATTCAGAGTGTAAAAGGAAAGGTTGTTATTGATGCCTTCAGATTGATCA
  ATGCTAATATGATGGTCTTAGGACATGAACCAAGACAAACAACTTCGAATCTGGGTCACTTAAACAAGCC
  ATCTATCCAGGCATTAATTCATGGACTAAACAGACATTATTACTCCATTACTATTAACTATCGGAAAAAT
  GAACTGGAACAGAAGATGTTGCTAAATTTGCATAAGAAGAGTTGGATGGAAGGTTTGACACTTCAGGACT
  ACAGTGAACATTGTAAACACAATGAATCAGTGGTAAAGGAGATGTTGGAATTAGCCAAGAATTACAATAA
  GGCTGTAGAAGAAGAAGATAAGATGACACCTGAACAGCTGGCAATAAAGAATGTTGGCAAGCAGGACCCC
  AAACGTCATTTGGAGGAACATGTGGATGTACTTATGACCTCAAATATTGTCCAGTGTTTAGCAGCTATGT
  TGGATACTGTCGTATTTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209829 representing NM_005805
  Red=Cloning site Green=Tags(s)
  
  MDRLLRLGGGMPGLGQGPPTDAPAVDTAEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVRVI
  DVFAMPQSGTGVSVEAVDPVFQAKMLDMLKQTGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALSERA
  VAVVVDPIQSVKGKVVIDAFRLINANMMVLGHEPRQTTSNLGHLNKPSIQALIHGLNRHYYSITINYRKN
  ELEQKMLLNLHKKSWMEGLTLQDYSEHCKHNESVVKEMLELAKNYNKAVEEEDKMTPEQLAIKNVGKQDP
  KRHLEEHVDVLMTSNIVQCLAAMLDTVVFK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005805
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005805.2, NP_005796.1
• RefSeq Size: 1278
• RefSeq ORF: 933
• Locus ID: 10213
• Domains: JAB_MPN
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012]