MOS (NM_005372) Human Tagged ORF Clone

CAT#: RG210144

MOS (GFP-tagged) - Human v-mos Moloney murine sarcoma viral oncogene homolog (MOS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MOS
• Synonyms: MSV
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210144 representing NM_005372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGCCCCTGGCCCTACGCCCCTACCTCCGGAGCGAGTTTTCCCCATCGGTGGACGCGCGGCCCT
  GCAGCAGTCCCTCAGAGCTACCTGCGAAGCTGCTTCTGGGGGCCACTCTTCCTCGGGCCCCGCGGCTGCC
  GCGCCGGCTGGCCTGGTGCTCCATTGACTGGGAGCAGGTGTGCTTGCTGCAGAGGCTGGGAGCTGGAGGG
  TTTGGCTCGGTGTACAAGGCGACTTACCGCGGTGTTCCTGTGGCCATAAAGCAAGTGAACAAGTGCACCA
  AGAACCGACTAGCATCTCGGCGGAGTTTCTGGGCTGAGCTCAACGTAGCAAGGCTGCGCCACGATAACAT
  CGTGCGCGTGGTGGCTGCCAGCACGCGCACGCCCGCAGGGTCCAATAGCCTAGGGACCATCATCATGGAG
  TTCGGTGGCAACGTCACTTTACACCAAGTCATCTATGGCGCCGCCGGCCACCCTGAGGGGGACGCAGGGG
  AGCCTCACTGCCGCACTGGAGGACAGTTAAGTTTGGGAAAGTGTCTCAAGTACTCACTAGATGTTGTGAA
  CGGCCTGCTCTTCCTCCACTCGCAAAGCATTGTGCACTTGGACCTGAAGCCCGCGAACATCTTGATCAGT
  GAGCAGGATGTCTGTAAAATTAGTGACTTCGGTTGCTCTGAGAAGTTGGAAGATCTGCTGTGCTTCCAGA
  CACCCTCTTACCCTCTAGGAGGCACATACACCCACCGCGCCCCGGAGCTCCTGAAAGGAGAGGGCGTGAC
  GCCTAAAGCCGACATTTATTCCTTTGCCATCACTCTCTGGCAAATGACTACCAAGCAGGCGCCGTATTCG
  GGGGAGCGGCAGCACATACTGTACGCGGTGGTGGCCTACGACCTGCGCCCGTCCCTCTCCGCTGCCGTCT
  TCGAGGACTCGCTCCCCGGGCAGCGCCTTGGGGACGTCATCCAGCGCTGCTGGAGACCCAGCGCGGCGCA
  GAGGCCGAGCGCGCGGCTGCTTTTGGTGGATCTCACCTCTTTGAAAGCTGAACTCGGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210144 representing NM_005372
  Red=Cloning site Green=Tags(s)
  
  MPSPLALRPYLRSEFSPSVDARPCSSPSELPAKLLLGATLPRAPRLPRRLAWCSIDWEQVCLLQRLGAGG
  FGSVYKATYRGVPVAIKQVNKCTKNRLASRRSFWAELNVARLRHDNIVRVVAASTRTPAGSNSLGTIIME
  FGGNVTLHQVIYGAAGHPEGDAGEPHCRTGGQLSLGKCLKYSLDVVNGLLFLHSQSIVHLDLKPANILIS
  EQDVCKISDFGCSEKLEDLLCFQTPSYPLGGTYTHRAPELLKGEGVTPKADIYSFAITLWQMTTKQAPYS
  GERQHILYAVVAYDLRPSLSAAVFEDSLPGQRLGDVIQRCWRPSAAQRPSARLLLVDLTSLKAELG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005372
• ORF Size: 1040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005372.1, NP_005363.1
• RefSeq Size: 1041 bp
• RefSeq ORF: 1041 bp
• Locus ID: 4342
• Cytogenetics: 8q12.1
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation, Regulation of actin cytoskeleton
• Gene Summary: 'MOS is a serine/threonine kinase that activates the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (MAP2K1; MIM 176872) (Prasad et al., 2008 [PubMed 18246541]).[supplied by OMIM, Jul 2009]'