GPR15 (NM_005290) Human Tagged ORF Clone

CAT#: RG210145

  • TrueORF®

GPR15 (GFP-tagged) - Human G protein-coupled receptor 15 (GPR15)


  "NM_005290" in other vectors (6)

Reconstitution Protocol

USD 460.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol GPR15
Synonyms BOB
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG210145 representing NM_005290
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCAGAAGAAACTTCAGTTTATTTGGATTATTACTATGCTACGAGCCCAAACTCTGACATCAGGG
AGACCCACTCCCATGTTCCTTACACCTCTGTCTTCCTTCCAGTCTTTTACACAGCTGTGTTCCTGACTGG
AGTGCTGGGGAACCTTGTTCTCATGGGAGCGTTGCATTTCAAACCCGGCAGCCGAAGACTGATCGACATC
TTTATCATCAATCTGGCTGCCTCTGACTTCATTTTTCTTGTCACATTGCCTCTCTGGGTGGATAAAGAAG
CATCTCTAGGACTGTGGAGGACGGGCTCCTTCCTGTGCAAAGGGAGCTCCTACATGATCTCCGTCAATAT
GCACTGCAGTGTCCTCCTGCTCACTTGCATGAGTGTTGACCGCTACCTGGCCATTGTGTGGCCAGTCGTA
TCCAGGAAATTCAGAAGGACAGACTGTGCATATGTAGTCTGTGCCAGCATCTGGTTTATCTCCTGCCTGC
TGGGGTTGCCTACTCTTCTGTCCAGGGAGCTCACGCTGATTGATGATAAGCCATACTGTGCAGAGAAAAA
GGCAACTCCAATTAAACTCATATGGTCCCTGGTGGCCTTAATTTTCACCTTTTTTGTCCCTTTGTTGAGC
ATTGTGACCTGCTACTGTTGCATTGCAAGGAAGCTGTGTGCCCATTACCAGCAATCAGGAAAGCACAACA
AAAAGCTGAAGAAATCTATAAAGATCATCTTTATTGTCGTGGCAGCCTTTCTTGTCTCCTGGCTGCCCTT
CAATACTTTCAAGTTCCTGGCCATTGTCTCTGGGTTGCGGCAAGAACACTATTTACCCTCAGCTATTCTT
CAGCTTGGTATGGAGGTGAGTGGACCCTTGGCATTTGCCAACAGCTGTGTCAACCCTTTCATTTACTATA
TCTTCGACAGCTACATCCGCCGGGCCATTGTCCACTGCTTGTGCCCTTGCCTGAAAAACTATGACTTTGG
GAGTAGCACTGAGACATCAGATAGTCACCTCACTAAGGCTCTCTCCACCTTCATTCATGCAGAAGATTTT
GCCAGGAGGAGGAAGAGGTCTGTGTCACTCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG210145 representing NM_005290
Red=Cloning site Green=Tags(s)

MDPEETSVYLDYYYATSPNSDIRETHSHVPYTSVFLPVFYTAVFLTGVLGNLVLMGALHFKPGSRRLIDI
FIINLAASDFIFLVTLPLWVDKEASLGLWRTGSFLCKGSSYMISVNMHCSVLLLTCMSVDRYLAIVWPVV
SRKFRRTDCAYVVCASIWFISCLLGLPTLLSRELTLIDDKPYCAEKKATPIKLIWSLVALIFTFFVPLLS
IVTCYCCIARKLCAHYQQSGKHNKKLKKSIKIIFIVVAAFLVSWLPFNTFKFLAIVSGLRQEHYLPSAIL
QLGMEVSGPLAFANSCVNPFIYYIFDSYIRRAIVHCLCPCLKNYDFGSSTETSDSHLTKALSTFIHAEDF
ARRRKRSVSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005290
ORF Size 1081 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005290.1, NP_005281.1
RefSeq Size 1083 bp
RefSeq ORF 1083 bp
Locus ID 2838
Cytogenetics 3q11.2
Protein Families Druggable Genome, GPCR, Transmembrane
Gene Summary 'This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012]'

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