RPA4 (NM_013347) Human Tagged ORF Clone

CAT#: RG210239

RPA4 (GFP-tagged) - Human replication protein A4, 30kDa (RPA4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RPA4
• Synonyms: HSU24186
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210239 representing NM_013347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGAGTGGGTTTGGGAGCTATGGCAGCATTTCTGCTGCTGATGGAGCGAGTGGAGGCAGTGACC
  AACTGTGTGAGAGAGATGCAACTCCTGCTATTAAGACCCAAAGACCTAAGGTCCGAATTCAGGACGTTGT
  ACCGTGTAATGTGAACCAGCTTCTCAGCTCTACTGTGTTTGACCCTGTGTTCAAGGTTAGGGGAATTATA
  GTTTCCCAGGTCTCCATCGTGGGGGTAATCAGAGGGGCAGAGAAGGCTTCAAATCACATTTGTTACAAAA
  TTGATGATATGACCGCGAAACCAATCGAGGCCCGACAGTGGTTTGGTAGAGAGAAAGTCAAGCAGGTGAC
  TCCATTGTCAGTCGGAGTATATGTCAAAGTGTTTGGTATCCTCAAATGTCCCACGGGAACAAAGAGCCTT
  GAGGTATTGAAAATTCATGTCCTAGAGGACATGAACGAGTTCACCGTGCATATTCTGGAAACGGTCAATG
  CACACATGATGCTGGATAAAGCCCGTCGTGATACCACTGTAGAAAGTGTGCCTGTGTCTCCATCAGAAGT
  GAATGATGCTGGGGATAACGATGAGAGTCACCGCAATTTCATCCAGGACGAAGTGCTGCGTTTGATTCAT
  GAGTGTCCTCATCAGGAAGGGAAGAGCATCCATGAGCTCCGGGCTCAGCTCTGCGACCTTAGCGTCAAGG
  CCATCAAGGAAGCGATTGATTATCTGACCGTTGAGGGCCACATCTATCCCACTGTGGATCGGGAGCATTT
  TAAGTCTGCTGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210239 representing NM_013347
  Red=Cloning site Green=Tags(s)
  
  MSKSGFGSYGSISAADGASGGSDQLCERDATPAIKTQRPKVRIQDVVPCNVNQLLSSTVFDPVFKVRGII
  VSQVSIVGVIRGAEKASNHICYKIDDMTAKPIEARQWFGREKVKQVTPLSVGVYVKVFGILKCPTGTKSL
  EVLKIHVLEDMNEFTVHILETVNAHMMLDKARRDTTVESVPVSPSEVNDAGDNDESHRNFIQDEVLRLIH
  ECPHQEGKSIHELRAQLCDLSVKAIKEAIDYLTVEGHIYPTVDREHFKSAD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013347
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013347.1, NP_037479.1
• RefSeq Size: 1560
• RefSeq ORF: 786
• Locus ID: 29935
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair
• Gene Summary: This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication. [provided by RefSeq, Apr 2010]