SHARP2 (BHLHE40) (NM_003670) Human Tagged ORF Clone

CAT#: RG210294

BHLHE40 (GFP-tagged) - Human basic helix-loop-helix family, member e40 (BHLHE40)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BHLHE40
• Synonyms: BHLHB2; Clast5; DEC1; HLHB2; SHARP-2; SHARP2; STRA13; Stra14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210294 representing NM_003670
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGATCCCCAGCGCGCAACCACCCCCCGCCTGCCTGCCCAAAGCACCGGGACTGGAGCACGGAG
  ACCTACCAGGGATGTACCCTGCCCACATGTACCAAGTGTACAAGTCAAGACGGGGAATAAAGCGGAGCGA
  GGACAGCAAGGAGACCTACAAATTGCCGCACCGGCTCATCGAGAAAAAGAGACGTGACCGGATTAACGAG
  TGCATCGCCCAGCTGAAGGATCTCCTACCCGAACATCTCAAACTTACAACTTTGGGTCACTTGGAAAAAG
  CAGTGGTTCTTGAACTTACCTTGAAGCATGTGAAAGCACTAACAAACCTAATTGATCAGCAGCAGCAGAA
  AATCATTGCCCTGCAGAGTGGTTTACAAGCTGGTGAGCTGTCAGGGAGAAATGTCGAAACAGGTCAAGAG
  ATGTTCTGCTCAGGTTTCCAGACATGTGCCCGGGAGGTGCTTCAGTATCTGGCCAAGCACGAGAACACTC
  GGGACCTGAAGTCTTCGCAGCTTGTCACCCACCTCCACCGGGTGGTCTCGGAGCTGCTGCAGGGTGGTAC
  CTCCAGGAAGCCATCAGACCCAGCTCCCAAAGTGATGGACTTCAAGGAAAAACCCAGCTCTCCGGCCAAA
  GGTTCGGAAGGTCCTGGGAAAAACTGCGTGCCAGTCATCCAGCGGACTTTCGCTCACTCGAGTGGGGAGC
  AGAGCGGCAGCGACACGGACACAGACAGTGGCTATGGAGGAGAATCGGAGAAGGGCGACTTGCGCAGTGA
  GCAGCCGTGCTTCAAAAGTGACCACGGACGCAGGTTCACGATGGGAGAAAGGATCGGCGCAATTAAGCAA
  GAGTCCGAAGAACCCCCCACAAAAAAGAACCGGATGCAGCTTTCGGATGATGAAGGCCATTTCACTAGCA
  GTGACCTGATCAGCTCCCCGTTCCTGGGCCCACACCCACACCAGCCTCCTTTCTGCCTGCCCTTCTACCT
  GATCCCACCTTCAGCGACTGCCTACCTGCCCATGCTGGAGAAGTGCTGGTATCCCACCTCAGTGCCAGTG
  CTATACCCAGGCCTCAACGCCTCTGCCGCAGCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTC
  CCTTGCTCATGCCCCAGAGACTCCCTTCTCCCTTGCCAGCTCATCCGTCCGTCGACTCTTCTGTCTTGCT
  CCAAGCTCTGAAGCCAATCCCCCCTTTAAACTTAGAAACCAAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210294 representing NM_003670
  Red=Cloning site Green=Tags(s)
  
  MERIPSAQPPPACLPKAPGLEHGDLPGMYPAHMYQVYKSRRGIKRSEDSKETYKLPHRLIEKKRRDRINE
  CIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHVKALTNLIDQQQQKIIALQSGLQAGELSGRNVETGQE
  MFCSGFQTCAREVLQYLAKHENTRDLKSSQLVTHLHRVVSELLQGGTSRKPSDPAPKVMDFKEKPSSPAK
  GSEGPGKNCVPVIQRTFAHSSGEQSGSDTDTDSGYGGESEKGDLRSEQPCFKSDHGRRFTMGERIGAIKQ
  ESEEPPTKKNRMQLSDDEGHFTSSDLISSPFLGPHPHQPPFCLPFYLIPPSATAYLPMLEKCWYPTSVPV
  LYPGLNASAAALSSFMNPDKISAPLLMPQRLPSPLPAHPSVDSSVLLQALKPIPPLNLETKD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003670
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003670.1, NP_003661.1
• RefSeq Size: 2922
• RefSeq ORF: 1239
• Locus ID: 8553
• Domains: HLH, ORANGE
• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014]