MSL2L1 (MSL2) (NM_018133) Human Tagged ORF Clone

CAT#: RG210829

MSL2 (GFP-tagged) - Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MSL2
• Synonyms: MSL-2; MSL2L1; RNF184
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210829 representing NM_018133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCCGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTACGACCCCGGAG
  ACCCCAAGGCGTTTACTGAGATTAACAGGCTCTTGCCTTACTTCCGACAGTCCCTTTCGTGCTGTGTTTG
  CGGACATTTGCTACAAGATCCTATTGCACCCACCAACTCCACCTGCCAACATTATGTCTGCAAAACTTGT
  AAAGGCAAGAAAATGATGATGAAACCTTCCTGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAAAACA
  AGCAGTTAAGCATCCTAGTGAACTGCTACAAAAAACTATGCGAGTATATAACACAGACTACACTGGCACG
  GGATATAATAGAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGATGGATCATTGTTTTGT
  GAGGAGACAGAAAAACCCTCAGATTCATCCTTTACTTTGTGTTTGACACATTCCCCTTTACCTTCAACCT
  CAGAACCCACAACTGATCCTCAAGCTAGTTTATCTCCAATGTCTGAAAGCACCCTCAGCATTGCTATTGG
  CAGTTCTGTTATCAATGGTTTGCCTACTTATAATGGGCTTTCAATAGATAGATTTGGTATAAATATTCCT
  TCACCTGAACATTCAAATACGATTGACGTATGTAATACTGTTGACATAAAAACTGAGGATCTGTCTGACA
  GCCTGCCACCCGTTTGTGACACAGTAGCCACTGACTTATGTTCCACAGGCATTGATATCTGCAGTTTCAG
  TGAAGATATAAAACCTGGAGACTCTCTGTTACTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
  TCAAATACAGAGGTCTGTTGCCCTAATTTGCAGCCGAACTTGGAAGCCACTGTATCCAATGGACCTTTTC
  TGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTTATGTCCACCAGTCCTGCACTTCATGGGTTATC
  ATGTACAGCAGCAACTCCGAAGATAGCAAAATTGAATAGAAAACGATCCAGATCAGAGAGTGACAGTGAG
  AAAGTTCAGCCACTTCCAATTTCTACCATTATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACAG
  TGAAACGGGAGAGCAAAATTTCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACAACACCTAAAAT
  CAGCAAAACTGTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
  TCTAAAACCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATTCCTAGTCATCATTTTA
  TGCCAGGAAGTCCTACCAAGACTGTGTACAAAAAACCCCAGGAAAAGAAAGGGTGTAAATGTGGGCGTGC
  TACTCAAAATCCAAGTGTTCTTACATGCCGAGGCCAACGCTGCCCTTGCTACTCTAACCGCAAAGCCTGC
  TTAGATTGTATATGTCGTGGCTGCCAAAACTCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTG
  CCGTGCCAGAAAAGGCCTTGGAGCAGACCAGGCTCACTTTGGGCATTAACGTGACTAGCATTGCTGTGCG
  TAACGCTAGTACCAGCACCAGTGTAATAAATGTCACAGGGTCCCCAGTAACGACGTTTTTAGCTGCCAGT
  ACACATGATGATAAAAGTTTGGATGAAGCTATAGACATGAGATTCGACTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210829 representing NM_018133
  Red=Cloning site Green=Tags(s)
  
  MNPVNATALYISASRLVLNYDPGDPKAFTEINRLLPYFRQSLSCCVCGHLLQDPIAPTNSTCQHYVCKTC
  KGKKMMMKPSCSWCKDYEQFEENKQLSILVNCYKKLCEYITQTTLARDIIEAVDCSSDILALLNDGSLFC
  EETEKPSDSSFTLCLTHSPLPSTSEPTTDPQASLSPMSESTLSIAIGSSVINGLPTYNGLSIDRFGINIP
  SPEHSNTIDVCNTVDIKTEDLSDSLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETV
  SNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSE
  KVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSH
  SKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKAC
  LDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAAS
  THDDKSLDEAIDMRFDC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018133
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018133.2, NP_060603.2
• RefSeq Size: 4696
• RefSeq ORF: 1734
• Locus ID: 55167
• Protein Families: Druggable Genome
• Gene Summary: Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histine H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1. [UniProtKB/Swiss-Prot Function]