LONP2 (NM_031490) Human Tagged ORF Clone

CAT#: RG210875

LONP2 (GFP-tagged) - Human lon peptidase 2, peroxisomal (LONP2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LONP2
• Synonyms: LONP; LONPL; PLON; PSLON
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210875 representing NM_031490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCAGTGAGCCCCATCCAGATCCCCAGTCGCCTCCCGCTGCTGCTCACCCACGAGGGCGTCCTGC
  TGCCCGGCTCCACCATGCGCACCAGCGTGGACTCGGCCCGCAACCTGCAGCTGGTGCGGAGCCGCCTTCT
  GAAGGGCACGTCGCTGCAAAGCACCATCCTGGGCGTCATCCCCAACACGCCTGACCCCGCCAGCGACGCG
  CAGGACCTGCCGCCGCTGCACAGGATTGGCACAGCTGCACTGGCCGTTCAGGTTGTGGGCAGTAACTGGC
  CCAAGCCCCACTACACTCTGTTGATTACAGGCCTATGCCGTTTCCAGATTGTACAGGTCTTAAAAGAGAA
  GCCATATCCCATTGCTGAAGTGGAGCAGTTGGACCGACTTGAGGAGTTTCCCAACACCTGTAAAATGAGG
  GAGGAGCTAGGAGAACTATCAGAGCAGTTTTACAAATATGCAGTACAATTGGTTGAAATGTTGGATATGT
  CTGTCCCTGCAGTTGCTAAATTGAGACGTCTTTTAGATAGTCTTCCAAGGGAAGCTTTACCAGACATCTT
  GACATCAATTATCCGAACAAGCAACAAAGAGAAACTCCAGATTTTAGATGCTGTGAGCCTAGAGGAGCGG
  TTCAAGATGACTATACCACTGCTTGTCAGACAAATTGAAGGCCTGAAATTGCTTCAAAAAACCAGAAAAC
  CCAAGCAAGATGATGATAAGAGGGTTATAGCAATACGCCCTATTAGGAGAATTACACATATCTCAGGTAC
  TTTAGAAGATGAAGATGAAGATGAAGATAATGATGACATTGTCATGCTAGAGAAAAAAATACGAACATCT
  AGTATGCCAGAGCAGGCCCATAAAGTCTGTGTCAAAGAGATAAAGAGACTCAAAAAAATGCCTCAGTCAA
  TGCCAGAATATGCTCTGACTAGAAATTATTTGGAACTTATGGTAGAACTTCCTTGGAACAAAAGTACAAC
  TGACCGCCTGGACATTAGGGCAGCCCGGATTCTTCTGGATAATGACCATTACGCCATGGAAAAATTGAAG
  AAAAGAGTACTGGAATACTTGGCTGTCAGACAGCTCAAAAATAACCTGAAGGGCCCAATCCTATGCTTTG
  TTGGCCCTCCTGGAGTTGGTAAAACAAGTGTGGGAAGATCAGTGGCCAAGACTCTAGGTCGAGAGTTCCA
  CAGGATTGCACTTGGAGGAGTATGTGATCAGTCTGACATTCGAGGACACAGGCGCACCTATGTTGGCAGC
  ATGCCTGGTCGCATCATCAACGGCTTGAAGACTGTGGGAGTGAACAACCCAGTGTTCCTATTAGATGAGG
  TTGACAAACTGGGAAAAAGTCTACAGGGTGATCCAGCAGCAGCTCTGCTTGAGGTGTTGGATCCTGAACA
  AAACCATAACTTCACAGATCATTATCTAAATGTGGCCTTTGACCTTTCTCAAGTTCTTTTTATAGCTACT
  GCCAACACCACTGCTACCATTCCAGCTGCCTTGTTGGACAGAATGGAGATCATTCAGGTTCCAGGTTATA
  CACAGGAGGAGAAGATAGAGATTGCCCATAGGCACTTGATCCCCAAGCAGCTGGAACAACATGGGCTGAC
  TCCACAGCAGATTCAGATACCCCAGGTCACCACTCTTGACATCATCACCAGGTATACCAGAGAGGCAGGG
  GTTCGTTCTCTGGATAGAAAACTTGGGGCCATTTGCCGAGCTGTGGCCGTGAAGGTGGCAGAAGGACAGC
  ATAAGGAAGCCAAGTTGGACCGTTCTGATGTGACTGAGAGAGAAGGTTGCAGAGAACACATCTTAGAAGA
  TGAAAAACCTGAATCTATCAGTGACACTACTGACTTGGCTCTACCACCTGAAATGCCGATTTTGATTGAT
  TTCCATGCTCTGAAAGACATCCTTGGGCCCCCGATGTATGAAATGGAGGTATCTCAGCGTTTGAGTCAGC
  CAGGAGTAGCAATAGGTTTGGCTTGGACTCCCTTAGGTGGAGAAATCATGTTCGTGGAGGCGAGTCGAAT
  GGATGGCGAGGGCCAGTTAACTCTGACCGGCCAGCTCGGGGACGTGATGAAGGAGTCCGCCCACCTCGCT
  ATCAGCTGGCTCCGCAGCAACGCAAAGAAGTACCAGCTGACCAATGCTTTTGGAAGTTTTGATCTTCTTG
  ACAACACAGACATCCATCTGCACTTCCCAGCTGGAGCTGTCACAAAAGATGGACCATCTGCTGGAGTTAC
  CATAGTAACCTGTCTCGCCTCACTTTTTAGTGGGCGGCTGGTACGTTCAGATGTAGCCATGACTGGAGAA
  ATTACACTGAGAGGTCTTGTTCTTCCAGTGGGTGGAATTAAAGACAAAGTGCTGGCGGCACACAGAGCGG
  GACTGAAGCAAGTCATTATTCCTCGGAGAAATGAAAAAGACCTTGAGGGAATCCCAGGCAACGTACGACA
  GGATTTAAGTTTTGTCACAGCAAGCTGCCTGGATGAGGTTCTTAATGCAGCTTTTGATGGTGGCTTTACT
  GTCAAGACCAGACCTGGTCTGTTAAATAGCAAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210875 representing NM_031490
  Red=Cloning site Green=Tags(s)
  
  MSSVSPIQIPSRLPLLLTHEGVLLPGSTMRTSVDSARNLQLVRSRLLKGTSLQSTILGVIPNTPDPASDA
  QDLPPLHRIGTAALAVQVVGSNWPKPHYTLLITGLCRFQIVQVLKEKPYPIAEVEQLDRLEEFPNTCKMR
  EELGELSEQFYKYAVQLVEMLDMSVPAVAKLRRLLDSLPREALPDILTSIIRTSNKEKLQILDAVSLEER
  FKMTIPLLVRQIEGLKLLQKTRKPKQDDDKRVIAIRPIRRITHISGTLEDEDEDEDNDDIVMLEKKIRTS
  SMPEQAHKVCVKEIKRLKKMPQSMPEYALTRNYLELMVELPWNKSTTDRLDIRAARILLDNDHYAMEKLK
  KRVLEYLAVRQLKNNLKGPILCFVGPPGVGKTSVGRSVAKTLGREFHRIALGGVCDQSDIRGHRRTYVGS
  MPGRIINGLKTVGVNNPVFLLDEVDKLGKSLQGDPAAALLEVLDPEQNHNFTDHYLNVAFDLSQVLFIAT
  ANTTATIPAALLDRMEIIQVPGYTQEEKIEIAHRHLIPKQLEQHGLTPQQIQIPQVTTLDIITRYTREAG
  VRSLDRKLGAICRAVAVKVAEGQHKEAKLDRSDVTEREGCREHILEDEKPESISDTTDLALPPEMPILID
  FHALKDILGPPMYEMEVSQRLSQPGVAIGLAWTPLGGEIMFVEASRMDGEGQLTLTGQLGDVMKESAHLA
  ISWLRSNAKKYQLTNAFGSFDLLDNTDIHLHFPAGAVTKDGPSAGVTIVTCLASLFSGRLVRSDVAMTGE
  ITLRGLVLPVGGIKDKVLAAHRAGLKQVIIPRRNEKDLEGIPGNVRQDLSFVTASCLDEVLNAAFDGGFT
  VKTRPGLLNSKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031490
• ORF Size: 2556 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031490.2, NP_113678.2
• RefSeq Size: 4342
• RefSeq ORF: 2559
• Locus ID: 83752
• Domains: Lon_C, AAA, AAA
• Protein Families: Druggable Genome, Protease
• Gene Summary: In human, peroxisomes function primarily to catalyze fatty acid beta-oxidation and, as a by-product, produce hydrogen peroxide and superoxide. The protein encoded by this gene is an ATP-dependent protease that likely plays a role in maintaining overall peroxisome homeostasis as well as proteolytically degrading peroxisomal proteins damaged by oxidation. The protein has an N-terminal Lon N substrate recognition domain, an ATPase domain, a proteolytic domain, and, in some isoforms, a C-terminal peroxisome targeting sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2017]