PTGIS (NM_000961) Human Tagged ORF Clone

CAT#: RG211030

PTGIS (GFP-tagged) - Human prostaglandin I2 (prostacyclin) synthase (PTGIS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTGIS
• Synonyms: CYP8; CYP8A1; PGIS; PTGI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211030 representing NM_000961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGGGCCGCGCTCCTCGGCCTCCTGGCCGCACTGTTGCTGCTGCTGCTACTGAGCCGCCGCCGCA
  CGCGGCGACCTGGTGAGCCTCCCCTGGACCTGGGCAGCATCCCCTGGTTGGGGTATGCCTTGGACTTTGG
  AAAAGATGCTGCCAGCTTCCTCACGAGGATGAAGGAGAAGCACGGTGACATCTTTACTATACTGGTTGGG
  GGCAGGTATGTCACCGTTCTCCTGGACCCACACTCCTACGACGCGGTGGTGTGGGAGCCTCGCACCAGGC
  TCGACTTCCATGCCTATGCCATCTTCCTCATGGAGAGGATTTTTGATGTGCAGCTTCCACATTACAGCCC
  CAGTGATGAAAAGGCCAGGATGAAACTGACTCTTCTCCACAGAGAGCTCCAGGCACTCACAGAAGCCATG
  TATACCAACCTCCATGCAGTGCTGTTGGGCGATGCTACAGAAGCAGGCAGTGGCTGGCACGAGATGGGTC
  TCCTCGACTTCTCCTACAGCTTCCTGCTCAGAGCCGGCTACCTGACTCTTTACGGAATTGAGGCGCTGCC
  ACGCACCCATGAAAGCCAGGCCCAGGACCGCGTCCACTCAGCTGATGTCTTCCACACCTTTCGCCAGCTC
  GACCGGCTGCTCCCCAAACTGGCCCGTGGCTCCCTGTCAGTGGGGGACAAGGACCACATGTGCAGTGTCA
  AAAGTCGCCTGTGGAAGCTGCTATCCCCAGCCAGGCTGGCCAGGCGGGCCCACCGGAGCAAATGGCTGGA
  GAGTTACCTGCTGCACCTGGAGGAGATGGGTGTGTCAGAGGAGATGCAGGCACGGGCCCTGGTGCTGCAG
  CTGTGGGCCACACAGGGGAATATGGGTCCCGCTGCCTTCTGGCTCCTGCTCTTCCTTCTCAAGAATCCTG
  AAGCCCTGGCTGCTGTCCGCGGAGAGCTCGAGAGTATCCTTTGGCAAGCGGAGCAGCCTGTCTCGCAGAC
  GACCACTCTCCCACAGAAGGTTCTAGACAGCACACCTGTGCTTGATAGCGTGCTGAGTGAGAGCCTCAGG
  CTTACAGCTGCCCCCTTCATCACCCGCGAGGTTGTGGTGGACCTGGCCATGCCCATGGCAGACGGGCGAG
  AATTCAACCTGCGACGTGGTGACCGCCTCCTCCTCTTCCCCTTCCTGAGCCCCCAGAGAGACCCAGAAAT
  CTACACAGACCCAGAGGTATTTAAATACAACCGATTCCTGAACCCTGACGGATCAGAGAAGAAAGACTTT
  TACAAGGATGGGAAACGGCTGAAGAATTACAACATGCCCTGGGGGGCGGGGCACAATCACTGCCTGGGGA
  GGAGTTATGCGGTCAACAGCATCAAACAATTTGTGTTCCTTGTGCTGGTGCACTTGGACTTGGAGCTGAT
  CAACGCAGATGTGGAGATCCCTGAGTTTGACCTCAGCAGGTACGGCTTCGGTCTGATGCAGCCGGAACAC
  GACGTGCCCGTCCGCTACCGCATCCGCCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211030 representing NM_000961
  Red=Cloning site Green=Tags(s)
  
  MAWAALLGLLAALLLLLLLSRRRTRRPGEPPLDLGSIPWLGYALDFGKDAASFLTRMKEKHGDIFTILVG
  GRYVTVLLDPHSYDAVVWEPRTRLDFHAYAIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAM
  YTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQL
  DRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQ
  LWATQGNMGPAAFWLLLFLLKNPEALAAVRGELESILWQAEQPVSQTTTLPQKVLDSTPVLDSVLSESLR
  LTAAPFITREVVVDLAMPMADGREFNLRRGDRLLLFPFLSPQRDPEIYTDPEVFKYNRFLNPDGSEKKDF
  YKDGKRLKNYNMPWGAGHNHCLGRSYAVNSIKQFVFLVLVHLDLELINADVEIPEFDLSRYGFGLMQPEH
  DVPVRYRIRP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000961
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000961.3, NP_000952.1
• RefSeq Size: 5603 bp
• RefSeq ORF: 1503 bp
• Locus ID: 5740
• Cytogenetics: 20q13.13
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008]'