PCPTP1 (PTPRR) (NM_130846) Human Tagged ORF Clone

CAT#: RG211122

PTPRR (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, R (PTPRR), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRR
• Synonyms: EC-PTP; PCPTP1; PTP-SL; PTPBR7; PTPRQ
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211122 representing NM_130846
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCTTTACAGATTAAAAGAAAGATTTCAGCTTTCCTTAAGACAAGACAAAGAGAAAAACCAGGAGA
  TCCACCTATCGCCCATCACATTACAGCCAGCACTGTCCGAGGCAAAGACAGTCCACAGCATGGTCCAACC
  TGAGCAGGCCCCAAAGGTACTGAATGTTGTCGTGGACCCTCAAGGCCGAGGTGCTCCTGAGATCAGAGCT
  ACCACCGCTACCTCTGTTTGCCCTTCTCCTTTCAAAATGAAGCCCATAGGACTTCAAGAGAGAAGAGGGT
  CCAACGTATCTCTTACATTGGACATGAGTAGCTTGGGGAACATTGAACCCTTTGTGTCTATACCAACACC
  ACGGGAGAAGGTAGCAATGGAGTATCTGCAGTCAGCCAGCCGAATTCTCACAAGGTCTCAGCTGAGGGAC
  GTCGTGGCAAGTTCACATTTACTCCAAAGTGAATTCATGGAAATACCAATGAACTTTGTGGATCCCAAAG
  AAATTGATATTCCGCGTCATGGAACTAAAAATCGCTATAAGACCATTTTACCAAATCCCCTCAGCAGAGT
  GTGTTTAAGACCAAAAAATGTAACCGATTCATTGAGCACCTACATTAATGCTAATTATATTAGGGGCTAC
  AGTGGCAAGGAGAAAGCCTTCATTGCCACGCAGGGCCCCATGATCAACACCGTGGATGATTTCTGGCAGA
  TGGTTTGGCAGGAAGACAGCCCTGTGATTGTTATGATCACAAAACTCAAAGAAAAAAATGAGAAATGTGT
  GCTATACTGGCCGGAAAAGAGAGGGATATATGGAAAAGTTGAGGTTCTGGTTATCAGTGTAAATGAATGT
  GATAACTACACCATTCGAAACCTTGTCTTAAAGCAAGGAAGCCACACCCAACATGTGAAGCATTACTGGT
  ACACCTCATGGCCTGATCACAAGACTCCAGACAGTGCCCAGCCCCTCCTACAGCTCATGCTGGATGTAGA
  AGAAGACAGACTTGCTTCCCAGGGCCGAGGGCCTGTGGTTGTCCACTGCAGTGCAGGAATAGGTAGAACA
  GGGTGTTTTATTGCTACATCCATTGGCTGTCAACAGCTGAAAGAAGAAGGAGTTGTGGATGCACTAAGCA
  TTGTCTGCCAGCTTCGTATGGATAGAGGTGGAATGGTGCAAACCAGTGAGCAGTATGAATTTGTGCACCA
  TGCTCTGTGCCTGTATGAGAGCAGACTTTCAGCAGAGACTGTCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211122 representing NM_130846
  Red=Cloning site Green=Tags(s)
  
  MILYRLKERFQLSLRQDKEKNQEIHLSPITLQPALSEAKTVHSMVQPEQAPKVLNVVVDPQGRGAPEIRA
  TTATSVCPSPFKMKPIGLQERRGSNVSLTLDMSSLGNIEPFVSIPTPREKVAMEYLQSASRILTRSQLRD
  VVASSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNVTDSLSTYINANYIRGY
  SGKEKAFIATQGPMINTVDDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVISVNEC
  DNYTIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASQGRGPVVVHCSAGIGRT
  GCFIATSIGCQQLKEEGVVDALSIVCQLRMDRGGMVQTSEQYEFVHHALCLYESRLSAETVQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130846
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130846.1, NP_570897.1
• RefSeq Size: 2733 bp
• RefSeq ORF: 1239 bp
• Locus ID: 5801
• Cytogenetics: 12q15
• Domains: Y_phosphatase
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. Silencing of this gene has been associated with colorectal cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares a symbol (PTPRQ) with another gene, protein tyrosine phosphatase, receptor type, Q (GeneID 374462), which is also located on chromosome 12. [provided by RefSeq, May 2011]'