HYAL4 (NM_012269) Human Tagged ORF Clone

CAT#: RG211151

HYAL4 (GFP-tagged) - Human hyaluronoglucosaminidase 4 (HYAL4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HYAL4
• Synonyms: CSHY; HYAL-4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211151 representing NM_012269
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTATTATCTGAAGGACAGTTAAAGCTTTGTGTTGTTCAACCAGTACATCTCACTTCATGGCTCC
  TTATATTTTTTATTCTAAAGTCTATCTCTTGTCTAAAACCTGCTCGACTTCCAATTTATCAAAGGAAACC
  TTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTGATAAAATATAATTTAAGACTAAATTTGAAA
  ATGTTTCCTGTGATTGGAAGCCCACTGGCCAAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACA
  GATTGGGATACTATCCGTGGTATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAG
  TTTACAAGTACATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
  CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAAGATGTTTACA
  GACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCTACCGATATTGAATATTTAGC
  CAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAGGAAACCATCAAATTGGGAATTAAGAGCCGA
  CCCAAAGGCCTTTGGGGTTATTATTTATATCCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACT
  CTGGGTCATGCCCAGAAGACGAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGC
  TTTATATCCTTCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
  CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTATTTGTCTACA
  CAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGATCTAGTCAGCACCATAGGAGA
  AAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGAGACATGAATTTAACTTCATCCAAGGCCAAC
  TGTACAAAGGTGAAGCAGTTTGTGAGTTCTGATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTG
  AGGTATGCAGCCTTCACCTCTGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTA
  CCTTCACTTGAACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
  GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGATATGAAGGAG
  CTGATTGCAGAGAAATAAAGACGGCTGATGGCTGCTCTGGGGTTTCCCCTTCTCCTGGTTCACTAATGAC
  ACTTTGTCTACTGCTTTTAGCAAGTTATCGAAGCATTCAGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211151 representing NM_012269
  Red=Cloning site Green=Tags(s)
  
  MKVLSEGQLKLCVVQPVHLTSWLLIFFILKSISCLKPARLPIYQRKPFIAAWNAPTDQCLIKYNLRLNLK
  MFPVIGSPLAKARGQNVTIFYVNRLGYYPWYTSQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSG
  LAVIDWEYWRPQWARNWNSKDVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKSR
  PKGLWGYYLYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLGDSENILRFSKF
  RVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESAALGAAGIVIWGDMNLTSSKAN
  CTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIRKMWNAPSYLHLNPASYHIEASEDGEFTVKGK
  ASDTDLAVMADTFSCHCYQGYEGADCREIKTADGCSGVSPSPGSLMTLCLLLLASYRSIQL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012269
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012269.1, NP_036401.1
• RefSeq Size: 2414
• RefSeq ORF: 1446
• Locus ID: 23553
• Domains: Glyco_hydro_56
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• Gene Summary: This gene encodes a protein which is similar in structure to hyaluronidases but lacks hyaluronidase activity. The encoded protein acts as a chondroitin-sulfate-specific endo-beta-N-acetylgalactosaminidase; that is, it exhibits hydrolytic activity toward chondroitin sulfate chains and degrades them into oligosaccharides. Proteoglycans are formed by the covalent linkage of chondroitin sulfate chains to protein. Proteoglycans are ubiquitous components of the extracellular matrix of connective tissues and are also found at the surface of many cell types where they participate in a variety of cellular processes such as cell proliferation, differentiation, migration, cell-cell recognition, extracellular matrix deposition, and tissue morphogenesis. The expression of this gene is highest in testes and placenta. [provided by RefSeq, Apr 2019]