RGS6 (NM_004296) Human Tagged ORF Clone

CAT#: RG211189

RGS6 (GFP-tagged) - Human regulator of G-protein signaling 6 (RGS6), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RGS6
• Synonyms: GAP; HA117; S914
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211189 representing NM_004296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAAGGATCCGGGGATCAAAGAGCAGTGGGGGTTGCTGACCCAGAGGAGAGTTCTCCAAACATGA
  TCGTTTACTGCAAAATTGAAGACATCATTACAAAGATGCAAGATGACAAGACAGGGGGTGTGCCCATCAG
  AACAGTCAAGAGCTTTCTCTCCAAAATCCCCAGTGTCGTCACAGGTACTGACATTGTGCAGTGGCTTATG
  AAGAACCTTTCCATTGAGGACCCAGTTGAAGCAATACACTTGGGGAGCCTTATCGCTGCCCAGGGCTACA
  TCTTTCCAATCTCAGACCATGTTCTCACCATGAAGGATGATGGCACCTTTTATCGTTTCCAGGCTCCGTA
  CTTCTGGCCTTCGAACTGCTGGGAACCTGAAAACACTGACTATGCCATCTATCTCTGTAAGAGGACAATG
  CAAAATAAAGCAAGGCTGGAACTGGCAGATTATGAAGCAGAAAACTTAGCAAGACTCCAGAGGGCCTTTG
  CGAGGAAGTGGGAATTCATCTTTATGCAAGCAGAAGCACAAGTAAAGATTGACCGGAAAAAAGACAAGAC
  AGAAAGGAAAATTTTGGATAGTCAAGAACGAGCCTTTTGGGATGTCCACAGGCCTGTGCCAGGCTGTGTG
  AACACAACAGAAATGGATATCCGAAAATGTCGACGTTTGAAGAATCCACAAAAGGTTAAAAAGTCCGTGT
  ATGGCGTGACTGAAGAGTCCCAGGCACAGAGCCCGGTGCATGTACTCAGCCAACCAATCAGGAAAACAAC
  AAAAGAGGACATCCGGAAACAGATAACATTTTTGAACGCACAGATCGACAGACATTGTTTGAAAATGTCC
  AAAGTGGCTGAAAGTTTAATTGCCTACACGGAACAATATGTGGAATATGACCCTTTGATAACACCAGCTG
  AGCCATCCAACCCTTGGATCAGCGATGACGTTGCTTTGTGGGACATAGAGATGAGCAAAGAGCCCAGCCA
  ACAGCGAGTAAAAAGATGGGGCTTCTCTTTCGATGAGATATTGAAGGACCAGGTGGGGCGGGACCAGTTT
  CTACGATTCCTGGAGTCCGAATTCAGTTCAGAAAACCTCAGGTTCTGGCTGGCTGTCCAAGATCTTAAGA
  AACAACCCCTACAGGATGTGGCCAAGAGGGTAGAAGAAATCTGGCAAGAGTTTCTGGCTCCAGGGGCTCC
  AAGTGCAATCAACCTGGATTCTCACAGCTATGAGATAACCAGTCAAAATGTCAAAGATGGAGGGAGATAT
  ACATTTGAAGACGCCCAGGAGCACATCTACAAGCTGATGAAGAGTGACAGCTATGCCCGCTTCCTCCGGT
  CAAATGCTTACCAGGATTTGCTGCTGGCCAAGAAGAAGGGAAAGTCGCTGGCGGGCAAGCGCCTCACGGG
  CCTGATGCAGTCCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211189 representing NM_004296
  Red=Cloning site Green=Tags(s)
  
  MAQGSGDQRAVGVADPEESSPNMIVYCKIEDIITKMQDDKTGGVPIRTVKSFLSKIPSVVTGTDIVQWLM
  KNLSIEDPVEAIHLGSLIAAQGYIFPISDHVLTMKDDGTFYRFQAPYFWPSNCWEPENTDYAIYLCKRTM
  QNKARLELADYEAENLARLQRAFARKWEFIFMQAEAQVKIDRKKDKTERKILDSQERAFWDVHRPVPGCV
  NTTEMDIRKCRRLKNPQKVKKSVYGVTEESQAQSPVHVLSQPIRKTTKEDIRKQITFLNAQIDRHCLKMS
  KVAESLIAYTEQYVEYDPLITPAEPSNPWISDDVALWDIEMSKEPSQQRVKRWGFSFDEILKDQVGRDQF
  LRFLESEFSSENLRFWLAVQDLKKQPLQDVAKRVEEIWQEFLAPGAPSAINLDSHSYEITSQNVKDGGRY
  TFEDAQEHIYKLMKSDSYARFLRSNAYQDLLLAKKKGKSLAGKRLTGLMQSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004296
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004296.3, NP_004287.3
• RefSeq Size: 3105
• RefSeq ORF: 1419
• Locus ID: 9628
• Domains: RGS, DEP, G-gamma
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins, which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains, the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling, and may modulate neuronal, cardiovascular, lymphocytic activities, and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains, complete or incomplete GGL domains, and distinct C-terminal domains, have been described for this gene, however, the full-length nature of some of these variants is not known. [provided by RefSeq, Mar 2011]