PCDHGA1 (NM_031993) Human Tagged ORF Clone

CAT#: RG211317

PCDHGA1 (GFP-tagged) - Human protocadherin gamma subfamily A, 1 (PCDHGA1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCDHGA1
• Synonyms: PCDH-GAMMA-A1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211317 representing NM_031993
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTCAGAAAAAGCTGACTGGCTGCAGCAGGCTGATGCTTCTGTGTCTTTCTCTGGAGCTGCTGT
  TGGAAGCTGGGGCTGGGAATATTCACTACTCAGTGCCGGAAGAGACAGACAAAGGTTCCTTCGTAGGCAA
  CATCGCCAAGGACCTAGGGCTGCAACCCCAGGAGCTGGCAGATGGCGGAGTCCGCATCGTCTCCAGAGGT
  AGGATGCCGCTTTTCGCTCTGAATCCTAGAAGTGGCAGCTTGATCACCGCGCGCAGGATAGACCGGGAGG
  AGCTCTGCGCTCAGAGCATGCCGTGTCTCGTGAGTTTTAATATCCTTGTTGAGGATAAAATGAAGCTTTT
  TCCTGTTGAAGTAGAAATAATTGATATTAATGACAACACTCCCCAATTCCAGTTAGAGGAACTGGAGTTT
  AAAATGAATGAAATAACGACTCCAGGTACCAGAATCTCATTGCCTTTTGGGCAAGACCTTGATGTGGGTA
  TGAACTCACTCCAGAGCTACCAACTCAGCTCTAACCCTCATTTCTCCCTGGATGTGCAACAGGGAGCCGA
  TGGGCCTCAACATCCAGAGATGGTGCTGCAGAGTCCCTTAGACAGAGAAGAAGAAGCTGTCCACCACCTC
  ATCCTCACAGCTTCTGATGGGGGTGAACCAGTCCGTTCAGGGACCCTCAGAATTTACATTCAGGTGGTGG
  ATGCAAATGACAATCCTCCAGCATTTACTCAGGCACAATACCATATAAATGTCCCCGAAAACGTGCCGCT
  GGGTACTCAGCTGCTCATGGTAAATGCCACTGACCCTGATGAGGGAGCCAATGGGGAAGTAACGTACTCC
  TTTCACAATGTAGACCACAGAGTGGCCCAAATATTTCGTTTAGATTCTTACACAGGAGAAATATCAAATA
  AAGAACCACTAGATTTCGAAGAATACAAAATGTATTCAATGGAAGTTCAAGCCCAGGATGGTGCGGGGCT
  CATGGCTAAAGTTAAGGTACTGATCAAAGTTTTGGATGTAAATGATAATGCCCCAGAAGTGACCATCACC
  TCTGTCACCACTGCAGTTCCAGAAAACTTTCCTCCTGGGACCATAATTGCTCTTATCAGTGTGCATGACC
  AGGACTCAGGAGACAATGGCTACACCACATGTTTCATTCCTGGAAATTTACCCTTTAAATTGGAAAAGTT
  AGTTGATAATTATTACCGTTTAGTGACTGAAAGAACACTGGACAGAGAACTTATCTCTGGGTACAACATC
  ACAATAACAGCAATAGACCAAGGAACTCCAGCTCTATCTACTGAAACTCACATTTCACTACTAGTGACAG
  ATATCAATGACAACTCCCCAGTCTTCCATCAGGACTCCTACTCTGCCTACATTCCCGAAAACAACCCCAG
  AGGAGCCTCCATCTTCTCTGTGAGGGCCCACGACTTGGACAGCAATGAGAATGCACAAATCACTTACTCC
  CTAATAGAGGACACTATCCAGGGGGCACCCCTATCTGCCTACCTCTCCATCAACTCCGACACTGGGGTCC
  TGTATGCGCTGCGATCCTTCGACTATGAGCAGTTCCGGGACATGCAACTGAAAGTGATGGCGCGGGACAG
  TGGGGATCCGCCCCTCAGCAGCAACGTGTCTCTCAGCCTATTCCTGCTGGACCAGAACGACAACGCGCCC
  GAGATCCTGTACCCCGCCCTCCCCACAGATGGTTCTACCGGCGTGGAGCTGGCGCCCCTCTCCGCAGAGC
  CCGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACAGAGACTCGGGCCAGAACGCCTGGCTGTCCTACCG
  CCTGCTCAAGGCCAGCGAGCCGGGACTCTTCTCGGTGGGTCTGCACACGGGCGAGGTGCGCACGGCGCGA
  GCCCTGCTGGACAGAGACGCGCTCAAGCAGAGTCTCGTGGTGGCCGTCCAGGACCACGGCCAGCCCCCGC
  TCTCCGCCACTGTCACGCTCACCGTGGCCGTGGCCGACAGGATCTCCGACATCCTGGCCGACCTGGGCAG
  CCTCGAGCCCTCCGCCAAACCCAACGATTCGGACCTCACTCTGTACCTGGTGGTGGCGGCGGCCGCGGTC
  TCCTGCGTCTTCCTGGCCTTCGTCATCGTGCTGCTGGCGCACAGGCTGCGGCGCTGGCACAAGTCACGTC
  TGCTACAGGCTTCGGGAGGCGGCTTAGCGAGCATGCCCGGTTCGCACTTTGTGGGCGTGGACGGGGTTCG
  GGCTTTCCTGCAGACCTATTCCCACGAGGTCTCCCTCACTGCGGACTCGCGGAAGAGCCACCTGATTTTC
  CCCCAGCCCAACTATGCGGACACACTCATCAGCCAGGAGAGCTGTGAGAAAAAGGGTTTTCTATCAGCAC
  CCCAGTCTTTACTTGAAGACAAAAAGGAACCATTTTCTCAGGTAAACTTTTGTGATGAATGTATCAGCTA
  TCTAGAGAAAAATAATTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211317 representing NM_031993
  Red=Cloning site Green=Tags(s)
  
  MKIQKKLTGCSRLMLLCLSLELLLEAGAGNIHYSVPEETDKGSFVGNIAKDLGLQPQELADGGVRIVSRG
  RMPLFALNPRSGSLITARRIDREELCAQSMPCLVSFNILVEDKMKLFPVEVEIIDINDNTPQFQLEELEF
  KMNEITTPGTRISLPFGQDLDVGMNSLQSYQLSSNPHFSLDVQQGADGPQHPEMVLQSPLDREEEAVHHL
  ILTASDGGEPVRSGTLRIYIQVVDANDNPPAFTQAQYHINVPENVPLGTQLLMVNATDPDEGANGEVTYS
  FHNVDHRVAQIFRLDSYTGEISNKEPLDFEEYKMYSMEVQAQDGAGLMAKVKVLIKVLDVNDNAPEVTIT
  SVTTAVPENFPPGTIIALISVHDQDSGDNGYTTCFIPGNLPFKLEKLVDNYYRLVTERTLDRELISGYNI
  TITAIDQGTPALSTETHISLLVTDINDNSPVFHQDSYSAYIPENNPRGASIFSVRAHDLDSNENAQITYS
  LIEDTIQGAPLSAYLSINSDTGVLYALRSFDYEQFRDMQLKVMARDSGDPPLSSNVSLSLFLLDQNDNAP
  EILYPALPTDGSTGVELAPLSAEPGYLVTKVVAVDRDSGQNAWLSYRLLKASEPGLFSVGLHTGEVRTAR
  ALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADRISDILADLGSLEPSAKPNDSDLTLYLVVAAAAV
  SCVFLAFVIVLLAHRLRRWHKSRLLQASGGGLASMPGSHFVGVDGVRAFLQTYSHEVSLTADSRKSHLIF
  PQPNYADTLISQESCEKKGFLSAPQSLLEDKKEPFSQVNFCDECISYLEKNNS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031993
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031993.1, NP_114382.1
• RefSeq Size: 2472
• RefSeq ORF: 2472
• Locus ID: 56114
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]