OR13G1 (NM_001005487) Human Tagged ORF Clone

CAT#: RG211369

OR13G1 (GFP-tagged) - Human olfactory receptor, family 13, subfamily G, member 1 (OR13G1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OR13G1
• Synonyms: OR1-37
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211369 representing NM_001005487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCACAGCGTTGTAACTGAGTTCATTATTCTGGGCCTCACCAAAAAGCCTGAACTCCAGGGAATTA
  TCTTCCTCTTTTTTCTCATTGTCTATCTTGTGGCTTTTCTCGGCAACATGCTCATCATCATTGCCAAAAT
  CTATAACAACACCTTGCATACGCCCATGTATGTTTTCCTTCTGACACTGGCTGTTGTGGACATCATCTGC
  ACAACAAGCATCATACCGAAGATGCTGGGGACCATGCTAACATCAGAAAATACCATTTCATATGCAGGCT
  GCATGTCCCAGCTCTTCTTGTTCACATGGTCTCTGGGAGCTGAGATGGTTCTCTTCACCACCATGGCCTA
  TGACCGCTATGTGGCCATTTGTTTCCCTCTTCATTACAGTACTATTATGAACCACCATATGTGTGTAGCC
  TTGCTCAGCATGGTCATGGCTATTGCAGTCACCAATTCCTGGGTGCACACAGCTCTTATCATGAGGTTGA
  CTTTCTGTGGGCCAAACACCATTGACCACTTCTTCTGTGAGATACCCCCATTGCTGGCTTTGTCCTGTAG
  CCCTGTAAGAATCAATGAGGTGATGGTGTATGTTGCTGATATTACCCTGGCCATAGGGGACTTTATTCTT
  ACCTGCATCTCCTATGGTTTTATCATTGTTGCTATTCTCCGTATCCGCACAGTAGAAGGCAAGAGGAAGG
  CCTTCTCAACATGCTCATCTCATCTCACAGTGGTGACCCTTTACTATTCTCCTGTAATCTACACCTATAT
  CCGCCCTGCTTCCAGCTATACATTTGAAAGAGACAAGGTGGTAGCTGCACTCTATACTCTTGTGACTCCC
  ACATTAAACCCGATGGTGTACAGCTTCCAGAATAGGGAGATGCAGGCAGGAATTAGGAAGGTGTTTGCAT
  TTCTGAAACAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211369 representing NM_001005487
  Red=Cloning site Green=Tags(s)
  
  MNHSVVTEFIILGLTKKPELQGIIFLFFLIVYLVAFLGNMLIIIAKIYNNTLHTPMYVFLLTLAVVDIIC
  TTSIIPKMLGTMLTSENTISYAGCMSQLFLFTWSLGAEMVLFTTMAYDRYVAICFPLHYSTIMNHHMCVA
  LLSMVMAIAVTNSWVHTALIMRLTFCGPNTIDHFFCEIPPLLALSCSPVRINEVMVYVADITLAIGDFIL
  TCISYGFIIVAILRIRTVEGKRKAFSTCSSHLTVVTLYYSPVIYTYIRPASSYTFERDKVVAALYTLVTP
  TLNPMVYSFQNREMQAGIRKVFAFLKH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005487
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005487.1, NP_001005487.1
• RefSeq Size: 924 bp
• RefSeq ORF: 924 bp
• Locus ID: 441933
• Cytogenetics: 1q44
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]