ZNF274 (NM_133502) Human Tagged ORF Clone

CAT#: RG211914

ZNF274 (GFP-tagged) - Human zinc finger protein 274 (ZNF274), transcript variant ZNF274c

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF274
• Synonyms: HFB101; ZF2; ZKSCAN19; ZSCAN51
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211914 representing NM_133502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCAGGCTTCCGACGGCCTGGTCCTGTGAACCAGTGACCTTTGAAGATGTAACACTGGGTTTTA
  CCCCGGAAGAGTGGGGACTGCTGGACCTCAAACAGAAGTCCCTGTACAGGGAAGTGATGCTGGAGAACTA
  CAGGAACCTGGTCTCAGTGGAACATCAGCTTTCCAAACCAGATGTGGTATCTCAGTTAGAGGAGGCAGAA
  GATTTCTGGCCAGTGGAGAGAGGAATTCCTCAAGACACCATTCCAGAGTATCCTGAGCTCCAGCTGGACC
  CTAAATTGGATCCTCTTCCTGCTGAGAGTCCCCTAATGAACATTGAGGTTGTTGAGGTCCTCACACTGAA
  CCAGGAGGTGGCTGGTCCCCGGAATGCCCAGATCCAGGCCCTATATGCTGAAGATGGAAGCCTGAGTGCA
  GATGCCCCCAGTGAGCAGATCCAACAGCAGGGCAAGCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGT
  TCCGGCAGTTCCGTTATAAGGACATGACAGGTCCCCGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCA
  CCAGTGGCTACAGCCTAAGGCACGCTCCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTA
  GGTGCACTGCCTGTGAAGCTCCGGACATGGGTGGAATCGCAGCACCCAGAGAACTGCCAAGAGGTGGTGG
  CCCTGGTAGAGGGTGTGACCTGGATGTCTGAGGAGGAAGTACTTCCTGCAGGACAACCTGCCGAGGGCAC
  CACCTGCTGCCTCGAGGTCACTGCCCAGCAGGAGGAGAAGCAGGAGGATGCAGCCATCTGCCCAGTGACA
  GTGCTCCCTGAGGAGCCAGTGACCTTCCAGGATGTGGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGC
  TGGGCCCGACACAGAGGACCGAGTACCGCGATGTGATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGG
  GTGGGAGACTACACTGGAAAATAAAGAGTTAGCTCCAAATTCTGACATTCCTGAGGAAGAACCAGCCCCC
  AGCCTGAAAGTACAAGAATCCTCAAGGGATTGTGCCTTGTCCTCTACATTAGAAGATACCTTGCAGGGTG
  GGGTCCAGGAAGTCCAAGACACAGTGTTGAAGCAGATGGAGTCTGCTCAGGAAAAAGACCTTCCTCAGAA
  GAAGCACTTTGACAACCGTGAGTCCCAGGCAAACAGTGGTGCTCTTGACACAAACCAAGTTTCGCTCCAG
  AAAATTGACAACCCTGAGTCCCAGGCAAACAGTGGCGCTCTTGACACAAACCAAGTTTTGCTCCACAAAA
  TTCCTCCTAGAAAACGATTGCGCAAACGTGACTCACAAGTTAAAAGTATGAAACATAATTCACGTGTAAA
  AATTCATCAGAAGAGCTGTGAAAGGCAAAAGGCCAAGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGG
  AGTACTAAACAGATTACGTTTATAAGAATTCACAAGGGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTA
  AAATATTCCGGAACCCAAGATACTTTTCTGTGCATAAGAAAATCCATACCGGAGAGAGGCCCTATGTGTG
  TCAAGACTGTGGGAAAGGATTTGTTCAGAGCTCTTCCCTCACACAGCATCAGAGAGTTCATTCTGGAGAG
  AGACCATTTGAATGTCAGGAGTGTGGGAGGACCTTCAATGATCGCTCAGCCATCTCCCAGCACCTGAGGA
  CTCACACTGGCGCTAAGCCCTACAAGTGTCAGGACTGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCAT
  CAGACATCAGAGGACTCACACCGGGGAGCGCCCATATGCATGCAACAAATGTGGAAAGGCCTTCACCCAG
  AGCTCACACCTTATTGGGCACCAGAGAACCCACAATAGGACAAAGCGAAAGAAGAAACAGCCTACCTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211914 representing NM_133502
  Red=Cloning site Green=Tags(s)
  
  MASRLPTAWSCEPVTFEDVTLGFTPEEWGLLDLKQKSLYREVMLENYRNLVSVEHQLSKPDVVSQLEEAE
  DFWPVERGIPQDTIPEYPELQLDPKLDPLPAESPLMNIEVVEVLTLNQEVAGPRNAQIQALYAEDGSLSA
  DAPSEQIQQQGKHPGDPEAARQRFRQFRYKDMTGPREALDQLRELCHQWLQPKARSKEQILELLVLEQFL
  GALPVKLRTWVESQHPENCQEVVALVEGVTWMSEEEVLPAGQPAEGTTCCLEVTAQQEEKQEDAAICPVT
  VLPEEPVTFQDVAVDFSREEWGLLGPTQRTEYRDVMLETFGHLVSVGWETTLENKELAPNSDIPEEEPAP
  SLKVQESSRDCALSSTLEDTLQGGVQEVQDTVLKQMESAQEKDLPQKKHFDNRESQANSGALDTNQVSLQ
  KIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSR
  STKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHTGERPYVCQDCGKGFVQSSSLTQHQRVHSGE
  RPFECQECGRTFNDRSAISQHLRTHTGAKPYKCQDCGKAFRQSSHLIRHQRTHTGERPYACNKCGKAFTQ
  SSHLIGHQRTHNRTKRKKKQPTS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133502
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133502.1, NP_598009.1
• RefSeq Size: 2839
• RefSeq ORF: 1962
• Locus ID: 10782
• Domains: KRAB, LER, zf-C2H2
• Protein Families: Transcription Factors
• Protein Pathways: Neurotrophin signaling pathway
• Gene Summary: This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008]