Jagged 2 (JAG2) (NM_002226) Human Tagged ORF Clone

CAT#: RG211925

JAG2 (GFP-tagged) - Human jagged 2 (JAG2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: JAG2
• Synonyms: HJ2; SER2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211925 representing NM_002226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCGCAGGGCCGGGGGCGCCTTCCCCGGCGGCTGCTGCTGCTGCTGGCGCTCTGGGTGCAGGCGG
  CGCGGCCCATGGGCTATTTCGAGCTGCAGCTGAGCGCGCTGCGGAACGTGAACGGGGAGCTGCTGAGCGG
  CGCCTGCTGTGACGGCGACGGCCGGACAACGCGCGCGGGGGGCTGCGGCCACGACGAGTGCGACACGTAC
  GTGCGCGTGTGCCTTAAGGAGTACCAGGCCAAGGTGACGCCCACGGGGCCCTGCAGCTACGGCCACGGCG
  CCACGCCCGTGCTGGGCGGCAACTCCTTCTACCTGCCGCCGGCGGGCGCTGCGGGGGACCGAGCGCGGGC
  GCGGGCCCGGGCCGGCGGCGACCAGGACCCGGGCCTCGTCGTCATCCCCTTCCAGTTCGCCTGGCCGCGC
  TCCTTTACCCTCATCGTGGAGGCCTGGGACTGGGACAACGATACCACCCCGAATGAGGAGCTGCTGATCG
  AGCGAGTGTCGCATGCCGGCATGATCAACCCGGAGGACCGCTGGAAGAGCCTGCACTTCAGCGGCCACGT
  GGCGCACCTGGAGCTGCAGATCCGCGTGCGCTGCGACGAGAACTACTACAGCGCCACTTGCAACAAGTTC
  TGCCGGCCCCGCAACGACTTTTTCGGCCACTACACCTGCGACCAGTACGGCAACAAGGCCTGCATGGACG
  GCTGGATGGGCAAGGAGTGCAAGGAAGCTGTGTGTAAACAAGGGTGTAATTTGCTCCACGGGGGATGCAC
  CGTGCCTGGGGAGTGCAGGTGCAGCTACGGCTGGCAAGGGAGGTTCTGCGATGAGTGTGTCCCCTACCCC
  GGCTGCGTGCATGGCAGTTGTGTGGAGCCCTGGCAGTGCAACTGTGAGACCAACTGGGGCGGCCTGCTCT
  GTGACAAAGACCTGAACTACTGTGGCAGCCACCACCCCTGCACCAACGGAGGCACGTGCATCAACGCCGA
  GCCTGACCAGTACCGCTGCACCTGCCCTGACGGCTACTCGGGCAGGAACTGTGAGAAGGCTGAGCACGCC
  TGCACCTCCAACCCGTGTGCCAACGGGGGCTCTTGCCATGAGGTGCCGTCCGGCTTCGAATGCCACTGCC
  CATCGGGCTGGAGCGGGCCCACCTGTGCCCTTGACATCGATGAGTGTGCTTCGAACCCGTGTGCGGCCGG
  TGGCACCTGTGTGGACCAGGTGGACGGCTTTGAGTGCATCTGCCCCGAGCAGTGGGTGGGGGCCACCTGC
  CAGCTGGACGCCAATGAGTGTGAAGGGAAGCCATGCCTTAACGCTTTTTCTTGCAAAAACCTGATTGGCG
  GCTATTACTGTGATTGCATCCCGGGCTGGAAGGGCATCAACTGCCATATCAACGTCAACGACTGTCGCGG
  GCAGTGTCAGCATGGGGGCACCTGCAAGGACCTGGTGAACGGGTACCAGTGTGTGTGCCCACGGGGCTTC
  GGAGGCCGGCATTGCGAGCTGGAACGAGACAAGTGTGCCAGCAGCCCCTGCCACAGCGGCGGCCTCTGCG
  AGGACCTGGCCGACGGCTTCCACTGCCACTGCCCCCAGGGCTTCTCCGGGCCTCTCTGTGAGGTGGATGT
  CGACCTTTGTGAGCCAAGCCCCTGCCGGAACGGCGCTCGCTGCTATAACCTGGAGGGTGACTATTACTGC
  GCCTGCCCTGATGACTTTGGTGGCAAGAACTGCTCCGTGCCCCGCGAGCCGTGCCCTGGCGGGGCCTGCA
  GAGTGATCGATGGCTGCGGGTCAGACGCGGGGCCTGGGATGCCTGGCACAGCAGCCTCCGGCGTGTGTGG
  CCCCCATGGACGCTGCGTCAGCCAGCCAGGGGGCAACTTTTCCTGCATCTGTGACAGTGGCTTTACTGGC
  ACCTACTGCCATGAGAACATTGACGACTGCCTGGGCCAGCCCTGCCGCAATGGGGGCACATGCATCGATG
  AGGTGGACGCCTTCCGCTGCTTCTGCCCCAGCGGCTGGGAGGGCGAGCTCTGCGACACCAATCCCAACGA
  CTGCCTTCCCGATCCCTGCCACAGCCGCGGCCGCTGCTACGACCTGGTCAATGACTTCTACTGTGCGTGC
  GACGACGGCTGGAAGGGCAAGACCTGCCACTCACGCGAGTTCCAGTGCGATGCCTACACCTGCAGCAACG
  GTGGCACCTGCTACGACAGCGGCGACACCTTCCGCTGCGCCTGCCCCCCCGGCTGGAAGGGCAGCACCTG
  CGCCGTCGCCAAGAACAGCAGCTGCCTGCCCAACCCCTGTGTGAATGGTGGCACCTGCGTGGGCAGCGGG
  GCCTCCTTCTCCTGCATCTGCCGGGACGGCTGGGAGGGTCGTACTTGCACTCACAATACCAACGACTGCA
  ACCCTCTGCCTTGCTACAATGGTGGCATCTGTGTTGACGGCGTCAACTGGTTCCGCTGCGAGTGTGCACC
  TGGCTTCGCGGGGCCTGACTGCCGCATCAACATCGACGAGTGCCAGTCCTCGCCCTGTGCCTACGGGGCC
  ACGTGTGTGGATGAGATCAACGGGTATCGCTGTAGCTGCCCACCCGGCCGAGCCGGCCCCCGGTGCCAGG
  AAGTGATCGGGTTCGGGAGATCCTGCTGGTCCCGGGGCACTCCGTTCCCACACGGAAGCTCCTGGGTGGA
  AGACTGCAACAGCTGCCGCTGCCTGGATGGCCGCCGTGACTGCAGCAAGGTGTGGTGCGGATGGAAGCCT
  TGTCTGCTGGCCGGCCAGCCCGAGGCCCTGAGCGCCCAGTGCCCACTGGGGCAAAGGTGCCTGGAGAAGG
  CCCCAGGCCAGTGTCTGCGACCACCCTGTGAGGCCTGGGGGGAGTGCGGCGCAGAAGAGCCACCGAGCAC
  CCCCTGCCTGCCACGCTCCGGCCACCTGGACAATAACTGTGCCCGCCTCACCTTGCATTTCAACCGTGAC
  CACGTGCCCCAGGGCACCACGGTGGGCGCCATTTGCTCCGGGATCCGCTCCCTGCCAGCCACAAGGGCTG
  TGGCACGGGACCGCCTGCTGGTGTTGCTTTGCGACCGGGCGTCCTCGGGGGCCAGTGCCGTGGAGGTGGC
  CGTGTCCTTCAGCCCTGCCAGGGACCTGCCTGACAGCAGCCTGATCCAGGGCGCGGCCCACGCCATCGTG
  GCCGCCATCACCCAGCGGGGGAACAGCTCACTGCTCCTGGCTGTCACCGAGGTCAAGGTGGAGACGGTTG
  TTACGGGCGGCTCTTCCACAGGTCTGCTGGTGCCTGTGCTGTGTGGTGCCTTCAGCGTGCTGTGGCTGGC
  GTGCGTGGTCCTGTGCGTGTGGTGGACACGCAAGCGCAGGAAAGAGCGGGAGAGGAGCCGGCTGCCGCGG
  GAGGAGAGCGCCAACAACCAGTGGGCCCCGCTCAACCCCATCCGCAACCCCATCGAGCGGCCGGGGGGCC
  ACAAGGACGTGCTCTACCAGTGCAAGAACTTCACGCCGCCGCCGCGCAGGGCGGACGAGGCGCTGCCCGG
  GCCGGCCGGCCACGCGGCCGTCAGGGAGGATGAGGAGGACGAGGATCTGGGCCGCGGTGAGGAGGACTCC
  CTGGAGGCGGAGAAGTTCCTCTCACACAAATTCACCAAAGATCCTGGCCGCTCGCCGGGGAGGCCGGCCC
  ACTGGGCCTCAGGCCCCAAAGTGGACAACCGCGCGGTCAGGAGCATCAATGAGGCCCGCTACGCCGGCAA
  GGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211925 representing NM_002226
  Red=Cloning site Green=Tags(s)
  
  MRAQGRGRLPRRLLLLLALWVQAARPMGYFELQLSALRNVNGELLSGACCDGDGRTTRAGGCGHDECDTY
  VRVCLKEYQAKVTPTGPCSYGHGATPVLGGNSFYLPPAGAAGDRARARARAGGDQDPGLVVIPFQFAWPR
  SFTLIVEAWDWDNDTTPNEELLIERVSHAGMINPEDRWKSLHFSGHVAHLELQIRVRCDENYYSATCNKF
  CRPRNDFFGHYTCDQYGNKACMDGWMGKECKEAVCKQGCNLLHGGCTVPGECRCSYGWQGRFCDECVPYP
  GCVHGSCVEPWQCNCETNWGGLLCDKDLNYCGSHHPCTNGGTCINAEPDQYRCTCPDGYSGRNCEKAEHA
  CTSNPCANGGSCHEVPSGFECHCPSGWSGPTCALDIDECASNPCAAGGTCVDQVDGFECICPEQWVGATC
  QLDANECEGKPCLNAFSCKNLIGGYYCDCIPGWKGINCHINVNDCRGQCQHGGTCKDLVNGYQCVCPRGF
  GGRHCELERDKCASSPCHSGGLCEDLADGFHCHCPQGFSGPLCEVDVDLCEPSPCRNGARCYNLEGDYYC
  ACPDDFGGKNCSVPREPCPGGACRVIDGCGSDAGPGMPGTAASGVCGPHGRCVSQPGGNFSCICDSGFTG
  TYCHENIDDCLGQPCRNGGTCIDEVDAFRCFCPSGWEGELCDTNPNDCLPDPCHSRGRCYDLVNDFYCAC
  DDGWKGKTCHSREFQCDAYTCSNGGTCYDSGDTFRCACPPGWKGSTCAVAKNSSCLPNPCVNGGTCVGSG
  ASFSCICRDGWEGRTCTHNTNDCNPLPCYNGGICVDGVNWFRCECAPGFAGPDCRINIDECQSSPCAYGA
  TCVDEINGYRCSCPPGRAGPRCQEVIGFGRSCWSRGTPFPHGSSWVEDCNSCRCLDGRRDCSKVWCGWKP
  CLLAGQPEALSAQCPLGQRCLEKAPGQCLRPPCEAWGECGAEEPPSTPCLPRSGHLDNNCARLTLHFNRD
  HVPQGTTVGAICSGIRSLPATRAVARDRLLVLLCDRASSGASAVEVAVSFSPARDLPDSSLIQGAAHAIV
  AAITQRGNSSLLLAVTEVKVETVVTGGSSTGLLVPVLCGAFSVLWLACVVLCVWWTRKRRKERERSRLPR
  EESANNQWAPLNPIRNPIERPGGHKDVLYQCKNFTPPPRRADEALPGPAGHAAVREDEEDEDLGRGEEDS
  LEAEKFLSHKFTKDPGRSPGRPAHWASGPKVDNRAVRSINEARYAGKE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002226
• ORF Size: 3714 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002226.3, NP_002217.3
• RefSeq Size: 5077 bp
• RefSeq ORF: 3717 bp
• Locus ID: 3714
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Notch signaling pathway
• Gene Summary: 'The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'