SHPRH (NM_173082) Human Tagged ORF Clone

CAT#: RG212151

SHPRH (GFP-tagged) - Human SNF2 histone linker PHD RING helicase (SHPRH), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SHPRH
• Synonyms: bA545I5.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212151 representing NM_173082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCCGACGGAAACGTGCTCCTCCAGTGAGGGTAGATGAGGAAAAGAGGCAGCAGCTTCATTGGA
  ATATGCATGAGGACAGAAGGAATGAACCTATCATCATAAGTGATGATGACGAGCAGCCCTGCCCAGGTTC
  AGATACCTCTTCTGCTCATTATATCATTCTAAGTGATAGTCTAAAGGAAGAAGTGGCTCACAGAGATAAG
  AAGAGGTGTTCAAAAGTGGTGAGCTTCTCAAAACCAATTGAAAAAGAAGAGACTGTTGGTATTTTTTCCC
  CTTTGTCTGTAAAGTTAAATATTGTGATTTCTCCCTATCATTTTGATAATTCCTGGAAAGCATTTCTAGG
  AGAATTAACTCTTCAGCTTCTTCCTGCACAGAGTTTAATTGAAAATTTTTCCGAAAGGAGTATTACATTG
  ATGAGTTCAGAGTCAAGCAATCAGTTCCTGATTTATGTTCATTCAAAAGGTGAAGATGTAGAGAAACAAA
  AAAAAGAACCGATGAGTATTTGTGACAAGGGTATTCTGGTGGAGTCATCCTTCAGTGGTGAAATGTTAGA
  AGATTTGGGGTGGCTACAAAAGAAGAGAAGAATAAAACTCTATCAGAAACCAGAAGGAAATCACATTATC
  AAGGTTGGAATTTATCTTTTGGAAGCTGGCCTAGCAAAACTAGACTTCTTGAGTGATGCAAATTCAAGAA
  TGAAAAAGTTCAATCAGCTCATGAAGAAAGTAATGGAAAAGTTACACAATTCTATTATTCCAGATGTGTT
  GGAAGAGGATGAAGATGATCCGGAGAGTGAGCCAGAGGGACAAGACATTGACGAGCTGTATCACTTTGTG
  AAACAAACACATCAGCAAGAAACGCAGTCCATCCAAGTGGATGTCCAGCATCCTGCATTGATCCCTGTGT
  TGAGACCCTACCAAAGAGAGGCTGTCAATTGGATGCTACAACAAGAGTGTTTCAGAAGCAGTCCTGCTAC
  TGAAAGTGCCCTGCACTTCTTATGGCGAGAGATTGTTACATCTGAGGGTCTGAAACTCTACTATAATCCA
  TATACAGGCTGCATCATTCGTGAGTACCCAAATTCTGGGCCGCAGTTGCTTGGTGGAATTTTAGCAGATG
  AGATGGGTCTTGGAAAGACAGTGGAGGTTTTGGCTCTGATTCTGACACATACTCGACAAGATGTCAAGCA
  AGATGCTCTCACTCTTCCCGAGGGAAAAGTGGTGAATTATTTTATTCCGTCACATTATTTTGGAGGAAAA
  CTGAAAAAGACAGAAATCCAGAATATCGAATTTGAACCAAAAGAAAAAGTTCAATGCCCTCCTACACGTG
  TGATGATACTGACAGCTGTGAAAGAAATGAATGGAAAAAAAGGAGTGTCCATCCTTTCCATCTATAAGTA
  TGTCAGTTCTATATATAGATACGATGTTCAACGGAACAGGAGTCTTTTGAAACGGATGCTGAAATGTTTA
  ATTTTTGAAGGTCTCGTGAAACAGATCAAAGGCCATGGTTTTTCTGGGACTTTTACATTGGGAAAGAATT
  ACAAGGAAGAGGATATATGTGATAAAACAAAAAAGCAGGCAGTAGGGAGTCCAAGGAAAATTCAGAAAGA
  AACTAGAAAATCAGGGAACAAGGACACAGATTCTGAATACTTGCCATCAGACACCTCTGATGATGATGAT
  GATCCTTACTATTATTATTATAAGTCCAGGAGAAATCGCAGTAAATTGAGGAAAAAGCTTGTTCCATCCA
  CAAAAAAAGGAAAAAGTCAACCATTTATCAATCCCGATTCACAAGGTCACTGTCCAGCTACTAGCGACTC
  TGGAATAACTGATGTTGCTATGTCTAAAAGTACATGTATCTCTGAATTCAACCAAGAACATGAAACAGAG
  GACTGTGCTGAATCTCTAAATCATGCTGATAGTGATGTACCACCTTCTAATACCATGAGTCCCTTTAACA
  CCTCTGATTACCGCTTTGAGTGTATATGTGGTGAACTTGATCAGATAGATCGTAAGCCTCGTGTTCAATG
  CCTGAAGTGTCACCTGTGGCAACATGCAAAGTGTGTGAATTATGATGAGAAAAATCTGAAGATCAAGCCT
  TTTTACTGCCCCCACTGCCTTGTTGCAATGGAACCAGTGTCAACAAGAGCAACTCTGATCATCTCTCCAA
  GTTCCATCTGTCACCAGTGGGTGGATGAGATCAACAGGCATGTGAGGTCGTCATCTCTTCGAGTCTTGGT
  ATATCAAGGAGTGAAGAAAGATGGCTTTTTACAACCTCATTTTTTGGCAGAACAGGATATAGTTATCATT
  ACCTATGATGTACTGCGTTCAGAATTAAATTATGTCGATATCCCACATAGCAATAGTGAGGATGGGCGTC
  GCCTACGGAACCAGAAGCGCTATATGGCTATCCCGAGCCCCCTGGTAGCTGTGGAGTGGTGGAGGATCTG
  CCTTGATGAAGCTCAGATGGTTGAGTGTCCCACAGTAAAAGCTGCAGAAATGGCCCAGCGTTTGAGTGGG
  ATTAATCGATGGTGTATCAGTGGCACTCCAGTACAGAGAGGATTAGAGGATCTTTTTGGGTTAGTGGTCT
  TTCTTGGTATTGAACCTTACTGTGTCAAACACTGGTGGGTTCGACTTCTCTATCGGCCTTACTGCAAGAA
  GAATCCTCAGCATCTCTACAGCTTTATTGCCAAGATACTGTGGAGGTCTGCAAAGAAAGATGTGATTGAC
  CAAATCCAAATACCACCACAAACCGAAGAAATACACTGGCTCCACTTTTCTCCAGTGGAAAGGCATTTCT
  ATCACCGTCAGCATGAGGTGTGCTGCCAGGATGTGGTGGTAAAACTCAGGAAGATTTCTGACTGGGCTCT
  GAAGCTCAGCAGCCTAGACAGAAGGACTGTCACCTCTATCCTGTATCCATTGCTGAGGCTCAGACAGGCC
  TGCTGTCACCCACAGGCTGTTCGTGGAGAGTTCTTGCCACTCCAAAAAAGTTTTGAACAGTCTACCTTTT
  CTTTTAGCACCATGACAATGGAAGAGCTGCTGACATCTTTGCAGAAGAAATGTGGAACTGAATGTGAAGA
  AGCACATCGACAGCTAGTTTGTGCTCTCAATGGCTTAGCAGGCATTCATATTATTAAAGGTGAGTATGCC
  TTGGCAGCAGAATTGTACAGAGAAGTGTTGCGCTCCTCGGAGGAACACAAAGGAAAACTCAAAACTGATT
  CACTTCAAAGACTTCATGCTACCCATAACTTGATGGAATTGTTGATAGCCAGGCACCCAGGGATACCACC
  TACCTTGCGTGATGGCCGACTTGAGGAAGAGGCCAAACAGCTGCGAGAGCACTACATGAGCAAGTGTAAT
  ACAGAAGTTGCTGAAGCCCAGCAAGCTTTATATCCTGTGCAGCAGACCATCCATGAGCTTCAAAGAAAGA
  TTCATTCTAATTCTCCTTGGTGGCTAAATGTGATCCACAGAGCAATAGAATTTACTATTGATGAGGAGCT
  AGTTCAGCGAGTGCGAAATGAAATAACCAGCAACTACAAGCAACAAACTGGCAAGCTTTCTATGTCAGAG
  AAAGGTCTTCAGTTCTTACTTACAACACAAATGGAAGAGCTAAATAAATGCCAGAAGCTAGTAAGAGAGG
  CTGTAAAAAACCTGGAGGGACCTCCATCTCGTAATGTTATTGAGTCTGCAACAGTCTGTCACCTCCGACC
  AGCCAGACTTCCTCTCAACTGCTGTGTCTTTTGTAAAGCTGATGAATTGTTCACAGAGTATGAATCAAAG
  CTATTTTCCAACACAGTCAAAGGCCAGACTGCAATATTTGAGGAGATGATAGAAGATGAAGAAGGACTGG
  TGGATGATCGAGCACCTACCACCACCCGGGGTCTCTGGGCAATAAGTGAGACAGAGCGATCTATGAAAGC
  AATACTATCATTTGCAAAATCACATAGGTTTGATGTTGAATTTGTTGATGAAGGAAGCACTTCAATGGAT
  CTCTTTGAAGCATGGAAGAAGGAATATAAGTTGCTTCATGAATATTGGATGGCTCTGAGGAATCGTGTGT
  CTGCTGTTGATGAACTTGCAATGGCTACAGAACGACTAAGAGTGCGTGATCCTAGGGAGCCAAAGCCTAA
  TCCGCCTGTTCTTCATATCATTGAACCACATGAGGTAGAACAAAACCGAATAAAACTACTAAATGATAAA
  GCTGTTGCTACATCACAGCTTCAGAAAAAACTTGGGCAGCTTCTTTACCTAACTAATTTGGAGAAGTCTC
  AAGATAAAACATCGGGAGGTGTTAATCCAGAACCTTGCCCAATCTGTGCTCGACAGCTAGGAAAACAGTG
  GGCGGTACTGACCTGTGGTCACTGTTTCTGTAATGAATGCATTTCTATAATTATTGAACAATACAGCGTG
  GGATCTCACAGAAGCTCCATTAAGTGTGCAATCTGCCGCCAGACCACATCTCACAAAGAAATCTCGTATG
  TCTTTACCTCAGAGAAAGCAAACCAGGAGGAGGACATCCCTGTGAAGGGCAGCCATTCTACAAAAGTGGA
  AGCTGTGGTCAGAACTCTGATGAAAATACAGCTTAGAGATCCAGGGGCCAAAGCACTCGTTTTCTCAACG
  TGGCAAGATGTATTAGATATTATTTCAAAAGCTCTTACTGACAACAACATGGAATTTGCACAAATCAGTC
  GTGTTAAGACATTTCAGGAGAACCTTTCAGCATTTAAACGTGATCCCCAAATCAATATTTTGCTGCTGCC
  CCTGCACACAGGTTCTAATGGATTAACTATCATTGAAGCAACTCATGTTCTCTTGGTGGAGCCCATATTG
  AACCCTGCCCATGAGCTTCAGGCCATAGGGAGGGTGCACCGAATTGGACAGACAAAACCTACTATTGTAC
  ACAGATTCTTAATTAAAGCAACAATAGAAGAAAGAATGCAGGCAATGCTGAAAACTGCTGAGAGAAGTAT
  CTATATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212151 representing NM_173082
  Red=Cloning site Green=Tags(s)
  
  MSSRRKRAPPVRVDEEKRQQLHWNMHEDRRNEPIIISDDDEQPCPGSDTSSAHYIILSDSLKEEVAHRDK
  KRCSKVVSFSKPIEKEETVGIFSPLSVKLNIVISPYHFDNSWKAFLGELTLQLLPAQSLIENFSERSITL
  MSSESSNQFLIYVHSKGEDVEKQKKEPMSICDKGILVESSFSGEMLEDLGWLQKKRRIKLYQKPEGNHII
  KVGIYLLEAGLAKLDFLSDANSRMKKFNQLMKKVMEKLHNSIIPDVLEEDEDDPESEPEGQDIDELYHFV
  KQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQECFRSSPATESALHFLWREIVTSEGLKLYYNP
  YTGCIIREYPNSGPQLLGGILADEMGLGKTVEVLALILTHTRQDVKQDALTLPEGKVVNYFIPSHYFGGK
  LKKTEIQNIEFEPKEKVQCPPTRVMILTAVKEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCL
  IFEGLVKQIKGHGFSGTFTLGKNYKEEDICDKTKKQAVGSPRKIQKETRKSGNKDTDSEYLPSDTSDDDD
  DPYYYYYKSRRNRSKLRKKLVPSTKKGKSQPFINPDSQGHCPATSDSGITDVAMSKSTCISEFNQEHETE
  DCAESLNHADSDVPPSNTMSPFNTSDYRFECICGELDQIDRKPRVQCLKCHLWQHAKCVNYDEKNLKIKP
  FYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRSSSLRVLVYQGVKKDGFLQPHFLAEQDIVII
  TYDVLRSELNYVDIPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSG
  INRWCISGTPVQRGLEDLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVID
  QIQIPPQTEEIHWLHFSPVERHFYHRQHEVCCQDVVVKLRKISDWALKLSSLDRRTVTSILYPLLRLRQA
  CCHPQAVRGEFLPLQKSFEQSTFSFSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGEYA
  LAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLIARHPGIPPTLRDGRLEEEAKQLREHYMSKCN
  TEVAEAQQALYPVQQTIHELQRKIHSNSPWWLNVIHRAIEFTIDEELVQRVRNEITSNYKQQTGKLSMSE
  KGLQFLLTTQMEELNKCQKLVREAVKNLEGPPSRNVIESATVCHLRPARLPLNCCVFCKADELFTEYESK
  LFSNTVKGQTAIFEEMIEDEEGLVDDRAPTTTRGLWAISETERSMKAILSFAKSHRFDVEFVDEGSTSMD
  LFEAWKKEYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLHIIEPHEVEQNRIKLLNDK
  AVATSQLQKKLGQLLYLTNLEKSQDKTSGGVNPEPCPICARQLGKQWAVLTCGHCFCNECISIIIEQYSV
  GSHRSSIKCAICRQTTSHKEISYVFTSEKANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFST
  WQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKRDPQINILLLPLHTGSNGLTIIEATHVLLVEPIL
  NPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAERSIYI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173082
• ORF Size: 4977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173082.3, NP_775105.1
• RefSeq Size: 7010
• RefSeq ORF: 4980
• Locus ID: 257218
• Domains: SNF2_N, DEAD, helicase_C, RING, PHD, linker_histone
• Protein Families: Druggable Genome
• Gene Summary: SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]). [supplied by OMIM, Mar 2008]