OPCML (NM_001012393) Human Tagged ORF Clone

CAT#: RG212254

OPCML (GFP-tagged) - Human opioid binding protein/cell adhesion molecule-like (OPCML), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OPCML
• Synonyms: IGLON1; OBCAM; OPCM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212254 representing NM_001012393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCATCCTGCCTACTGGGTCGTCTTCTCGGCGACAACTGCCCTGCTCTTCATCCCAGGAGTGCCCG
  TGCGCAGCGGAGATGCCACCTTCCCCAAAGCTATGGACAACGTGACGGTCCGGCAGGGGGAGAGCGCCAC
  CCTCAGGTGTACCATAGATGACCGGGTAACCCGGGTGGCCTGGCTAAACCGCAGCACCATCCTCTACGCT
  GGGAATGACAAGTGGTCCATAGACCCTCGTGTGATCATCCTGGTCAATACACCAACCCAGTACAGCATCA
  TGATCCAAAATGTGGATGTGTATGACGAAGGTCCGTACACCTGCTCTGTGCAGACAGACAATCATCCCAA
  AACGTCCCGGGTTCACCTAATAGTGCAAGTTCCTCCTCAGATCATGAATATCTCCTCAGACATCACTGTG
  AATGAGGGAAGCAGTGTGACCCTGCTGTGTCTTGCTATTGGCAGACCAGAGCCAACTGTGACATGGAGAC
  ACCTGTCAGTCAAGGAAGGCCAGGGCTTTGTAAGTGAGGATGAGTACCTGGAGATCTCTGACATCAAGCG
  AGACCAGTCCGGGGAGTACGAATGCAGCGCGTTGAACGATGTCGCTGCGCCCGATGTGCGGAAAGTAAAA
  ATCACTGTAAACTATCCTCCCTATATCTCAAAAGCCAAGAACACTGGTGTTTCAGTCGGTCAGAAGGGCA
  TCCTGAGCTGTGAAGCCTCTGCAGTCCCCATGGCTGAATTCCAGTGGTTCAAGGAAGAAACCAGGTTAGC
  CACTGGTCTGGATGGAATGAGGATTGAAAACAAAGGCCGCATGTCCACTCTGACTTTCTTCAATGTTTCA
  GAAAAGGATTATGGGAACTATACTTGTGTGGCCACGAACAAGCTTGGGAACACCAATGCCAGCATCACAT
  TGTATGGGCCTGGAGCAGTCATTGATGGTGTAAACTCGGCCTCCAGAGCACTGGCTTGTCTCTGGCTATC
  AGGGACCCTCTTAGCCCACTTCTTCATCAAGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212254 representing NM_001012393
  Red=Cloning site Green=Tags(s)
  
  MYHPAYWVVFSATTALLFIPGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYA
  GNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITV
  NEGSSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVK
  ITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEETRLATGLDGMRIENKGRMSTLTFFNVS
  EKDYGNYTCVATNKLGNTNASITLYGPGAVIDGVNSASRALACLWLSGTLLAHFFIKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012393
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012393.1, NP_001012393.1
• RefSeq Size: 6413 bp
• RefSeq ORF: 1017 bp
• Locus ID: 4978
• Cytogenetics: 11q25
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the IgLON subfamily in the immunoglobulin protein superfamily of proteins. The encoded preprotein is proteolytically processed to generate the mature protein. This protein is localized in the plasma membrane and may have an accessory role in opioid receptor function. This gene has an ortholog in rat and bovine. The opioid binding-cell adhesion molecule encoded by the rat gene binds opioid alkaloids in the presence of acidic lipids, exhibits selectivity for mu ligands and acts as a GPI-anchored protein. Since the encoded protein is highly conserved in species during evolution, it may have a fundamental role in mammalian systems. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]