OPCML (NM_001012393) Human Untagged Clone

CAT#: SC301639

OPCML (untagged)-Human opioid binding protein/cell adhesion molecule-like (OPCML), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OPCML
• Synonyms: IGLON1; OBCAM; OPCM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001012393 edited
  ATGTACCATCCTGCCTACTGGGTCGTCTTCTCGGCGACAACTGCCCTGCTCTTCATCCCA
  GGAGTGCCCGTGCGCAGCGGAGATGCCACCTTCCCCAAAGCTATGGACAACGTGACGGTC
  CGGCAGGGGGAGAGCGCCACCCTCAGGTGTACCATAGATGACCGGGTAACCCGGGTGGCC
  TGGCTAAACCGCAGCACCATCCTCTACGCTGGGAATGACAAGTGGTCCATAGACCCTCGT
  GTGATCATCCTGGTCAATACACCAACCCAGTACAGCATCATGATCCAAAATGTGGATGTG
  TATGACGAAGGTCCGTACACCTGCTCTGTGCAGACAGACAATCATCCCAAAACGTCCCGG
  GTTCACCTAATAGTGCAAGTTCCTCCTCAGATCATGAATATCTCCTCAGACATCACTGTG
  AATGAGGGAAGCAGTGTGACCCTGCTGTGTCTTGCTATTGGCAGACCAGAGCCAACTGTG
  ACATGGAGACACCTGTCAGTCAAGGAAGGCCAGGGCTTTGTAAGTGAGGATGAGTACCTG
  GAGATCTCTGACATCAAGCGAGACCAGTCCGGGGAGTACGAATGCAGCGCGTTGAACGAT
  GTCGCTGCGCCCGATGTGCGGAAAGTAAAAATCACTGTAAACTATCCTCCCTATATCTCA
  AAAGCCAAGAACACTGGTGTTTCAGTCGGTCAGAAGGGCATCCTGAGCTGTGAAGCCTCT
  GCAGTCCCCATGGCTGAATTCCAGTGGTTCAAGGAAGAAACCAGGTTAGCCACTGGTCTG
  GATGGAATGAGGATTGAAAACAAAGGCCGCATGTCCACTCTGACTTTCTTCAATGTTTCA
  GAAAAGGATTATGGGAACTATACTTGTGTGGCCACGAACAAGCTTGGGAACACCAATGCC
  AGCATCACATTGTATGGGCCTGGAGCAGTCATTGATGGTGTAAACTCGGCCTCCAGAGCA
  CTGGCTTGTCTCTGGCTATCAGGGACCCTCTTAGCCCACTTCTTCATCAAGTTTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001012393 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGTAGCAGCAGC
  AGAAGCAGTAGCAAGCCCGGCAGCTGAGAGCACCGCAGCGTCGAGATGTACCATCCTGCC
  TACTGGGTCGTCTTCTCGGCGACAACTGCCCTGCTCTTCATCCCAGGAGTGCCCGTGCGC
  AGCGGAGATGCCACCTTCCCCAAAGCTATGGACAACGTGACGGTCCGGCAGGGGGAGAGC
  GCCACCCTCAGGTGTACCATAGATGACCGGGTAACCCGGGTGGCCTGGCTAAACCGCAGC
  ACCATCCTCTACGCTGGGAATGACAAGTGGTCCATAGACCCTCGTGTGATCATCCTGGTC
  AATACACCAACCCAGTACAGCATCATGATCCAAAATGTGGATGTGTATGACGAAGGTCCG
  TACACCTGCTCTGTGCAGACAGACAATCATCCCAAAACGTCCCGGGTTCACCTAATAGTG
  CAAGTTCCTCCTCAGATCATGAATATCTCCTCAGACATCACTGTGAATGAGGGAAGCAGT
  GTGACCCTGCTGTGTCTTGCTATTGGCAGACCAGAGCCAACTGTGACATGGAGACACCTG
  TCAGTCAAGGAAGGCCAGGGCTTTGTAAGTGAGGATGAGTACCTGGAGATCTCTGACATC
  AAGCGAGACCAGTCCGGGGAGTACGAATGCAGCGCGTTGAACGATGTCGCTGCGCCCGAT
  GTGCGGAAAGTAAAAATCACTGTAAACTATCCTNCCTATATCTCAAAGCCAAGAACACTG
  GGTGTTNCAGTCGGTCAGAAGGGCTCCTGAGCTGTGAAGCCTCTGCAGTCCCATGGCTGA
  ATTCCAGTGGGTCAAGGGAGAAACCAGTAGCCACTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001012393 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAATGGGGCTCATATTTTTTA
  TTTTTTAAACTGAGGGCCTGAAAGGTTTTCCAACTGAGGCAGGGCAGGTCTGCGATTGAT
  GCTGCATTCTCATCCTCTCTGTGGATCAGTCCCTGCAGCACACCTGTGCGGTCTGCAATG
  CCTTTATTTTGAATCCCAGGCAACTTTGCTGGATGAGTCCTTCTCGTCCCAGGAGACAAG
  ACAGCAAAGGTGGCTCAGCTCTTGTTTGTATGCTGGGTCTGCTTTGAACCTGAAAAGCTG
  GTGAAGTTCTTCCCCAAAGCCATTGGAGAAGCAGCTGCATCAAAGGAAACTGTGTCACTG
  ACACTTTCTCCCTTCTGTTTGCAGTGTTGTGTTAATCAAGTGCTTGAAAATTAAGTCAGT
  TGCTCAAGTGCCCTCAGCTTAGAAGAGCTTCCTGAAACAAATACCAGAGTTGTCAATACA
  CCCGCCATGTAATTGGCCTCCATCAGCTTTTTACTGGATAGTTTTCAAACAGATTAAACC
  AGAACTGGAGCAGAAAGGAGACTCTAGCTGAAGTCATCCCACTCAGGCGGGTGGACTCTT
  CACTTTGCATTGCAGAGACAGATGTGTTTTTCTCTCAAGTTCTTGCTGCTAAGGAAAGCC
  AGCCATAGGTTCCTGACATGTACTTTCTTGAAGGGTTGAGAGAAGCATAAAGGCTGGGCA
  CCTTTGTCCTTNCAGTGCAAAAGGAAACCACAGTGGCTATCAAACTTTTCTAGGCTGATC
  TGCTTCCAAGAAAAGTGTTTAAAAACACAGCTCTTCTGGAATATGAGNGACTGACCATAN
  CTGGCCTACTTGCCTATCTGGTAAGGCCCCCTTGGGTGGGATAGATACCANATTTTAAAG
  AGTCCAACACAGGGATTGCTTTGGGATTTGTAACCGGGGAAAACGTGGCTTTCACGAAGT
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001012393
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001012393.1, NP_001012393.1
• RefSeq Size: 6413 bp
• RefSeq ORF: 1017 bp
• Locus ID: 4978
• Cytogenetics: 11q25
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the IgLON subfamily in the immunoglobulin protein superfamily of proteins. The encoded preprotein is proteolytically processed to generate the mature protein. This protein is localized in the plasma membrane and may have an accessory role in opioid receptor function. This gene has an ortholog in rat and bovine. The opioid binding-cell adhesion molecule encoded by the rat gene binds opioid alkaloids in the presence of acidic lipids, exhibits selectivity for mu ligands and acts as a GPI-anchored protein. Since the encoded protein is highly conserved in species during evolution, it may have a fundamental role in mammalian systems. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, and lacks an alternate in-frame exon in the 3' coding region compared to variant 3. The encoded isoform (b) is shorter and has a distinct N-terminus compared to isoform c. The encoded isoform (b) may undergo proteolytic processing similar to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.