CENTG3 (AGAP3) (NM_031946) Human Tagged ORF Clone

CAT#: RG212440

AGAP3 (GFP-tagged) - Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AGAP3
• Synonyms: AGAP-3; CENTG3; cnt-g3; CRAG; MRIP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212440 representing NM_031946
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTCCAGGCGGGCGGGGGGCAGAGCCCGCAGCAGCAGCAGAGCCTGGCGGCTCCGGGGGGCGGCG
  GCGCTGCCGCGCAGCAGCTCGTCTGCGGCGGGCAGTTCGGCGGCGCGGGGCCCGGGGCCGGGGGCGGCGG
  CGGCCCCTCGCAGCAGCTGGCCGGCGGGCCCCCCCAGCAGTTCGCGCTCTCCAACTCCGCGGCCATCCGG
  GCCGAGATCCAGCGCTTCGAGTCCGTGCATCCCAATATCTACGCCATCTACGACCTGATCGAGCGCATCG
  AGGATTTGGCGCTGCAGAACCAGATCCGGGAGCACGTCATCTCCATCGAGGACTCGTTTGTGAACAGCCA
  GGAGTGGACGCTGAGCCGCTCCGTACCGGAGCTTAAAGTGGGCATAGTGGGGAACCTGTCTAGCGGGAAG
  TCAGCCCTGGTGCACCGCTATCTGACGGGGACCTATGTCCAGGAGGAGTCCCCTGAAGGGGGGCGGTTTA
  AGAAGGAGATTGTGGTGGATGGCCAGAGTTACCTGCTGCTGATCCGAGATGAAGGAGGCCCCCCTGAGCT
  CCAGTTTGCTGCCTGGGTGGATGCAGTGGTGTTTGTGTTCAGCCTGGAGGATGAAATCAGTTTCCAGACG
  GTGTACAACTACTTCCTGCGTCTCTGCAGCTTCCGCAACGCCAGCGAGGTGCCCATGGTGCTTGTGGGCA
  CGCAGGATGCCATCAGCGCTGCGAATCCCCGGGTTATCGACGACAGCAGAGCCCGCAAGCTCTCCACAGA
  TCTGAAGCGGTGCACCTACTATGAGACGTGCGCGACCTACGGGCTCAATGTGGAGCGTGTCTTCCAGGAC
  GTGGCCCAGAAGGTAGTGGCCTTGCGAAAGAAGCAGCAACTGGCCATCGGGCCCTGCAAGTCACTGCCCA
  ACTCGCCCAGCCACTCGGCCGTGTCCGCCGCCTCCATCCCGGCCGTGCACATCAACCAGGCCACGAATGG
  CGGCGGCAGCGCCTTCAGCGACTACTCGTCCTCAGTCCCCTCCACCCCCAGCATCAGCCAGCGGGAGCTG
  CGCATCGAGACCATCGCTGCCTCCTCCACCCCCACACCCATCCGAAAGCAGTCCAAGCGGCGCTCCAACA
  TCTTCACGTCTCGGAAGGGTGCTGACCTGGACCGGGAGAAGAAGGCTGCCGAGTGCAAGGTGGACAGCAT
  CGGGAGCGGCCGCGCCATCCCCATCAAGCAGGGGATCCTGCTAAAGCGGAGCGGCAAGTCCCTGAACAAG
  GAGTGGAAGAAGAAGTATGTGACGCTCTGTGACAACGGGCTGCTCACCTATCACCCCAGCCTGCATGATT
  ACATGCAGAACATCCACGGCAAGGAGATTGACCTGCTGCGGACAACGGTGAAAGTGCCAGGGAAGCGCCT
  GCCCCGAGCCACACCTGCCACAGCCCCGGGCACCAGCCCCCGTGCCAACGGGCTGTCCGTGGAGCGGAGT
  AACACACAGCTGGGTGGGGGCACAGGTGCCCCCCACTCGGCCAGCAGCGCATCCCTGCACTCTGAGCGCC
  CCCTCAGCAGCTCGGCCTGGGCTGGCCCGCGCCCTGAGGGGCTGCACCAGCGCTCCTGCTCCGTTTCCAG
  CGCCGACCAGTGGAGTGAGGCCACCACTTCCCTGCCCCCAGGCATGCAGCACCCTGCCAGTGGCCCAGCT
  GAGGTACTCAGTTCCAGCCCCAAGCTGGATCCTCCCCCATCTCCCCACTCCAACCGGAAGAAGCACCGGA
  GGAAAAAGAGCACCGGGACCCCCCGACCAGACGGCCCCAGCAGTGCTACTGAAGAGGCAGAGGAGTCGTT
  TGAATTTGTGGTGGTGTCCCTCACTGGGCAGACGTGGCACTTCGAGGCTTCAACGGCGGAGGAGCGGGAG
  CTGTGGGTTCAGAGTGTGCAGGCCCAGATCCTTGCCAGCCTGCAAGGCTGCCGCAGTGCCAAGGACAAGA
  CTCGACTGGGGAACCAGAACGCAGCTCTGGCTGTGCAGGCCGTCCGCACCGTCCGCGGCAACAGCTTTTG
  TATCGACTGCGATGCACCCAATCCAGACTGGGCCAGCCTGAACCTGGGTGCCCTGATGTGCATTGAGTGC
  TCAGGCATCCACCGACACCTGGGGGCTCACCTGTCCCGGGTGCGCTCCCTTGACCTCGATGACTGGCCGC
  CTGAGCTGCTGGCTGTCATGACTGCCATGGGCAATGCCCTCGCCAACAGCGTCTGGGAGGGGGCCTTGGG
  TGGCTACTCCAAGCCAGGGCCTGATGCCTGCAGAGAGGAGAAGGAACGCTGGATACGGGCCAAGTATGAA
  CAGAAGCTCTTCCTGGCCCCACTGCCAAGCTCAGATGTGCCACTGGGGCAGCAGCTGCTCCGGGCCGTGG
  TGGAAGATGACCTGCGGCTGTTGGTGATGCTCCTGGCACATGGCTCCAAAGAGGAGGTGAATGAGACCTA
  TGGGGACGGGGACGGGCGGACGGCTCTACATCTCTCCAGTGCCATGGCCAACGTTGTCTTCACGCAGCTG
  CTCATCTGGTACGGGGTGGACGTGAGGAGCCGGGACGCCCGGGGCCTGACTCCACTGGCATATGCTCGCC
  GGGCCGGCAGCCAGGAGTGTGCAGACATCTTGATCCAGCATGGCTGCCCTGGGGAGGGCTGTGGCTTAGC
  GCCTACCCCCAACAGAGAGCCTGCCAATGGCACCAACCCCTCTGCTGAGCTGCACCGTAGTCCTAGCCTC
  CTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212440 representing NM_031946
  Red=Cloning site Green=Tags(s)
  
  MNFQAGGGQSPQQQQSLAAPGGGGAAAQQLVCGGQFGGAGPGAGGGGGPSQQLAGGPPQQFALSNSAAIR
  AEIQRFESVHPNIYAIYDLIERIEDLALQNQIREHVISIEDSFVNSQEWTLSRSVPELKVGIVGNLSSGK
  SALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPELQFAAWVDAVVFVFSLEDEISFQT
  VYNYFLRLCSFRNASEVPMVLVGTQDAISAANPRVIDDSRARKLSTDLKRCTYYETCATYGLNVERVFQD
  VAQKVVALRKKQQLAIGPCKSLPNSPSHSAVSAASIPAVHINQATNGGGSAFSDYSSSVPSTPSISQREL
  RIETIAASSTPTPIRKQSKRRSNIFTSRKGADLDREKKAAECKVDSIGSGRAIPIKQGILLKRSGKSLNK
  EWKKKYVTLCDNGLLTYHPSLHDYMQNIHGKEIDLLRTTVKVPGKRLPRATPATAPGTSPRANGLSVERS
  NTQLGGGTGAPHSASSASLHSERPLSSSAWAGPRPEGLHQRSCSVSSADQWSEATTSLPPGMQHPASGPA
  EVLSSSPKLDPPPSPHSNRKKHRRKKSTGTPRPDGPSSATEEAEESFEFVVVSLTGQTWHFEASTAEERE
  LWVQSVQAQILASLQGCRSAKDKTRLGNQNAALAVQAVRTVRGNSFCIDCDAPNPDWASLNLGALMCIEC
  SGIHRHLGAHLSRVRSLDLDDWPPELLAVMTAMGNALANSVWEGALGGYSKPGPDACREEKERWIRAKYE
  QKLFLAPLPSSDVPLGQQLLRAVVEDDLRLLVMLLAHGSKEEVNETYGDGDGRTALHLSSAMANVVFTQL
  LIWYGVDVRSRDARGLTPLAYARRAGSQECADILIQHGCPGEGCGLAPTPNREPANGTNPSAELHRSPSL
  L
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031946
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031946.4, NP_114152.3
• RefSeq Size: 3247
• RefSeq ORF: 2736
• Locus ID: 116988
• Domains: ArfGap, PH, ANK, RAS, RAB
• Gene Summary: This gene encodes an essential component of the N-methyl-D-aspartate (NMDA) receptor signaling complex which mediates long-term potentiation in synapses by linking activation of NMDA receptor to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking. The encoded protein contains an N-terminal GTPase-like domain, a pleckstrin homology domain, an ArfGAP domain and several C-terminal ankryn repeat domains. [provided by RefSeq, Apr 2017]