Dnmt2 (TRDMT1) (NM_176083) Human Tagged ORF Clone

CAT#: RG212492

TRDMT1 (GFP-tagged) - Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TRDMT1
• Synonyms: DMNT2; DNMT2; M.HsaIIP; PuMet; RNMT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212492 representing NM_176083
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCCTGCGGGTGCTGGAGCTATACAGCGGCGTGGGCGGCATGCACCACGCGCTGAGAGAAAGCT
  GTATACCTGCACAAGTGGTGGCTGCCATTGATGTCAACACTGTCGCTAATGAAGTATACAAGTATAATTT
  TCCTCACACACAGTTACTTGCCAAGACGATTGAAGGCATTACACTCGAAGAGTTTGACAGATTATCTTTT
  GATATGATTTTAATGAGCCCTCCCTGCCAGCCATTCACAAGATTACAAAAATTACCAAAGTATATTCTTT
  TGGAAAATGTTAAAGGTTTTGAAGTATCTTCTACAAGAGACCTCTTGATACAAACAATAGAAAATTGTGG
  CTTTCAGTACCAAGAGTTTCTATTATCTCCAACCTCTCTTGGCATTCCAAATTCAAGGCTACGATATTTT
  CTTATTGCAAAGCTTCAGTCAGAGCCATTACCCTTTCAAGCCCCTGGTCAGGTACTGATGGAGTTCCCCA
  AAATTGAATCTGTACATCCACAAAAATATGCAATGGATGTAGAAAATAAAATTCAAGAAAAGAACGTTGA
  ACCAAATATTAGCTTTGATGGCAGCATACAGTGTTCTGGAAAAGATGCCATTCTTTTTAAGCTTGAAACT
  GCAGAAGAAATTCACAGGAAAAATCAACAAGATAGTGATCTCTCTGTGAAAATGCTAAAAGATTTTCTTG
  AAGATGACACTGACGTGAACCAGTATCTTTTACCACCAAAGTCATTGCTGCGATATGCTCTTCTGTTAGA
  CATTGTTCAGCCCACTTGTAGAAGGTCCGTGTGCTTTACCAAAGGATATGGAAGCTACATAGAAGGGACA
  GGGTCTGTGTTACAGACTGCAGAGGATGTGCAGGTTGAGAATATCTACAAATCCCTTACCAATTTGTCAC
  AAGAAGAACAGATAACAAAGCTGTTAATACTTAAACTGCGATATTTCACTCCTAAAGAAATAGCAAATCT
  CCTTGGATTTCCTCCAGAGTTCGGATTTCCTGAGAAGATAACAGTGAAACAGCGTTATCGCCTACTTGGA
  AATAGTCTCAACGTGCATGTAGTAGCTAAACTAATCAAAATCTTATATGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212492 representing NM_176083
  Red=Cloning site Green=Tags(s)
  
  MEPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSF
  DMILMSPPCQPFTRLQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYF
  LIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLET
  AEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGT
  GSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLG
  NSLNVHVVAKLIKILYE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176083
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176083.1, NP_788271.1
• RefSeq Size: 3467 bp
• RefSeq ORF: 1103 bp
• Locus ID: 1787
• Cytogenetics: 10p13
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]'