Dnmt2 (TRDMT1) (NM_176083) Human Untagged Clone

CAT#: SC307060

TRDMT1 (untagged)-Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRDMT1
• Synonyms: DMNT2; DNMT2; M.HsaIIP; PuMet; RNMT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_176083, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCCCTGCGGGTGCTGGAGCTATACAGCGGCGTGGGCGGCATGCACCACGCGCTGAGAGAAAGCT
  GTATACCTGCACAAGTGGTGGCTGCCATTGATGTCAACACTGTCGCTAATGAAGTATACAAGTATAATTT
  TCCTCACACACAGTTACTTGCCAAGACGATTGAAGGCATTACACTCGAAGAGTTTGACAGATTATCTTTT
  GATATGATTTTAATGAGCCCTCCCTGCCAGCCATTCACAAGATTACAAAAATTACCAAAGTATATTCTTT
  TGGAAAATGTTAAAGGTTTTGAAGTATCTTCTACAAGAGACCTCTTGATACAAACAATAGAAAATTGTGG
  CTTTCAGTACCAAGAGTTTCTATTATCTCCAACCTCTCTTGGCATTCCAAATTCAAGGCTACGATATTTT
  CTTATTGCAAAGCTTCAGTCAGAGCCATTACCCTTTCAAGCCCCTGGTCAGGTACTGATGGAGTTCCCCA
  AAATTGAATCTGTACATCCACAAAAATATGCAATGGATGTAGAAAATAAAATTCAAGAAAAGAACGTTGA
  ACCAAATATTAGCTTTGATGGCAGCATACAGTGTTCTGGAAAAGATGCCATTCTTTTTAAGCTTGAAACT
  GCAGAAGAAATTCACAGGAAAAATCAACAAGATAGTGATCTCTCTGTGAAAATGCTAAAAGATTTTCTTG
  AAGATGACACTGACGTGAACCAGTATCTTTTACCACCAAAGTCATTGCTGCGATATGCTCTTCTGTTAGA
  CATTGTTCAGCCCACTTGTAGAAGGTCCGTGTGCTTTACCAAAGGATATGGAAGCTACATAGAAGGGACA
  GGGTCTGTGTTACAGACTGCAGAGGATGTGCAGGTTGAGAATATCTACAAATCCCTTACCAATTTGTCAC
  AAGAAGAACAGATAACAAAGCTGTTAATACTTAAACTGCGATATTTCACTCCTAAAGAAATAGCAAATCT
  CCTTGGATTTCCTCCAGAGTTCGGATTTCCTGAGAAGATAACAGTGAAACAGCGTTATCGCCTACTTGGA
  AATAGTCTCAACGTGCATGTAGTAGCTAAACTAATCAAAATCTTATATGAATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_176083
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176083.2, NP_788271.1
• RefSeq Size: 7209 bp
• RefSeq ORF: 1104 bp
• Locus ID: 1787
• Cytogenetics: 10p13
• Protein Families: Druggable Genome
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (b) lacks an alternate in-frame exon compared to variant a. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.