B4GALNT1 (NM_001478) Human Tagged ORF Clone

CAT#: RG212605

B4GALNT1 (GFP-tagged) - Human beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4GALNT1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: B4GALNT1
• Synonyms: GALGT; GalNAc-T; GALNACT; SPG26
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212605 representing NM_001478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGGGCCGCCGGGCCCTGTGCGCTCTGGTCCTTCTGCTCGCCTGCGCCTCGCTGGGGCTCCTGT
  ACGCGAGCACCCGGGACGCGCCCGGCCTCCGGCTACCTCTTGCGCCGTGGGCGCCCCCGCAAAGCCCCCG
  CAGGCCCGAGCTGCCAGATCTTGCTCCTGAGCCCCGCTACGCACACATCCCGGTCAGGATCAAGGAGCAA
  GTAGTGGGGCTGCTGGCTTGGAACAACTGCAGTTGTGAGTCCAGTGGGGGGGGCCTCCCCCTCCCCTTCC
  AGAAACAAGTCCGAGCTATTGACCTCACCAAGGCCTTTGACCCTGCAGAGCTGAGGGCTGCCTCTGCCAC
  AAGAGAGCAGGAGTTCCAGGCCTTTCTGTCGAGGAGCCAGTCCCCAGCTGACCAGCTGCTCATAGCCCCT
  GCCAACTCCCCGCTCCAGTACCCCCTACAGGGTGTGGAAGTTCAGCCCCTCAGGAGCATCTTGGTGCCAG
  GGCTGAGCCTTCAGGCAGCTTCTGGTCAGGAGGTATACCAGGTGAACCTGACTGCCTCCCTAGGCACCTG
  GGACGTGGCAGGGGAAGTGACTGGAGTTACTCTCACTGGAGAGGGTCAGGCAGATCTCACCCTTGTCAGC
  CCAGGGCTGGACCAACTCAACAGGCAACTACAACTGGTCACTTACAGCAGCCGAAGCTACCAGACCAACA
  CAGCAGACACAGTCCGGTTCTCCACCGAGGGACATGAGGCTGCTTTCACTATCCGCATAAGACACCCGCC
  CAACCCTCGGCTGTACCCACCTGGGTCTCTACCCCAGGGAGCCCAGTACAACATCAGCGCTCTAGTCACG
  ATTGCCACCAAGACCTTCCTCCGTTATGATCGGCTACGGGCTCTCATCACCAGTATCCGCCGCTTCTACC
  CAACGGTTACCGTGGTCATCGCTGACGACAGCGACAAGCCAGAGCGCGTTAGTGGCCCCTACGTGGAACA
  CTATCTCATGCCCTTCGGCAAGGGCTGGTTCGCAGGCCGGAACCTGGCCGTGTCTCAAGTAACCACCAAG
  TACGTGCTGTGGGTGGACGACGACTTCGTCTTCACGGCGCGGACGCGGCTGGAGAGGCTTGTGGACGTGC
  TGGAGCGGACGCCGCTGGACCTGGTGGGGGGCGCGGTGCGCGAGATCTCCGGCTTTGCCACCACTTATCG
  GCAGCTGCTGAGCGTGGAGCCCGGCGCCCCAGGCCTCGGGAACTGCCTCCGGCAAAGGCGCGGCTTCCAC
  CACGAGCTCGTCGGCTTCCCAGGCTGCGTGGTCACCGACGGCGTGGTTAACTTCTTCCTGGCGCGGACTG
  ACAAGGTGCGCGAGGTCGGTTTCGACCCCCGCCTCAGCCGCGTGGCTCATCTGGAATTCTTCTTGGATGG
  GCTTGGTTCCCTTCGGGTTGGCTCCTGCTCCGACGTCGTGGTGGATCATGCATCCAAACTGAAGCTGCCT
  TGGACATCAAGGGATGCCGGAGCAGAGACTTACGCCCGGTACCGTTACCCAGGATCACTGGACGAGAGCC
  AGATGGCCAAACACCGGCTGCTCTTCTTCAAACACCGGCTGCAGTGCATGACCTCCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212605 representing NM_001478
  Red=Cloning site Green=Tags(s)
  
  MWLGRRALCALVLLLACASLGLLYASTRDAPGLRLPLAPWAPPQSPRRPELPDLAPEPRYAHIPVRIKEQ
  VVGLLAWNNCSCESSGGGLPLPFQKQVRAIDLTKAFDPAELRAASATREQEFQAFLSRSQSPADQLLIAP
  ANSPLQYPLQGVEVQPLRSILVPGLSLQAASGQEVYQVNLTASLGTWDVAGEVTGVTLTGEGQADLTLVS
  PGLDQLNRQLQLVTYSSRSYQTNTADTVRFSTEGHEAAFTIRIRHPPNPRLYPPGSLPQGAQYNISALVT
  IATKTFLRYDRLRALITSIRRFYPTVTVVIADDSDKPERVSGPYVEHYLMPFGKGWFAGRNLAVSQVTTK
  YVLWVDDDFVFTARTRLERLVDVLERTPLDLVGGAVREISGFATTYRQLLSVEPGAPGLGNCLRQRRGFH
  HELVGFPGCVVTDGVVNFFLARTDKVREVGFDPRLSRVAHLEFFLDGLGSLRVGSCSDVVVDHASKLKLP
  WTSRDAGAETYARYRYPGSLDESQMAKHRLLFFKHRLQCMTSQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001478
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001478.2, NP_001469.1
• RefSeq Size: 2885 bp
• RefSeq ORF: 1602 bp
• Locus ID: 2583
• Cytogenetics: 12q13.3
• Domains: Glycos_transf_2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• Gene Summary: 'GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]'