B4GALNT1 (NM_001478) Human Untagged Clone

CAT#: SC119240

B4GALNT1 (untagged)-Human beta-1,4-N-acetyl-galactosaminyl transferase 1 (B4GALNT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B4GALNT1
• Synonyms: GALGT; GalNAc-T; GALNACT; SPG26
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001478, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCTGGGCCGCCGGGCCCTGTGCGCTCTGGTCCTTCTGCTCGCCTGCGCCTCGCTGGGGCTCCTGT
  ACGCGAGCACCCGGGACGCGCCCGGCCTCCGGCTACCTCTTGCGCCGTGGGCGCCCCCGCAAAGCCCCCG
  CAGGCCCGAGCTGCCAGATCTTGCTCCTGAGCCCCGCTACGCACACATCCCGGTCAGGATCAAGGAGCAA
  GTAGTGGGGCTGCTGGCTTGGAACAACTGCAGTTGTGAGTCCAGTGGGGGGGGCCTCCCCCTCCCCTTCC
  AGAAACAAGTCCGAGCTATTGACCTCACCAAGGCCTTTGACCCTGCAGAGCTGAGGGCTGCCTCTGCCAC
  AAGAGAGCAGGAGTTCCAGGCCTTTCTGTCGAGGAGCCAGTCCCCAGCTGACCAGCTGCTCATAGCCCCT
  GCCAACTCCCCGCTCCAGTACCCCCTACAGGGTGTGGAAGTTCAGCCCCTCAGGAGCATCTTGGTGCCAG
  GGCTGAGCCTTCAGGCAGCTTCTGGTCAGGAGGTATACCAGGTGAACCTGACTGCCTCCCTAGGCACCTG
  GGACGTGGCAGGGGAAGTGACTGGAGTTACTCTCACTGGAGAGGGTCAGGCAGATCTCACCCTTGTCAGC
  CCAGGGCTGGACCAACTCAACAGGCAACTACAACTGGTCACTTACAGCAGCCGAAGCTACCAGACCAACA
  CAGCAGACACAGTCCGGTTCTCCACCGAGGGACATGAGGCTGCTTTCACTATCCGCATAAGACACCCGCC
  CAACCCTCGGCTGTACCCACCTGGGTCTCTACCCCAGGGAGCCCAGTACAACATCAGCGCTCTAGTCACG
  ATTGCCACCAAGACCTTCCTCCGTTATGATCGGCTACGGGCTCTCATCACCAGTATCCGCCGCTTCTACC
  CAACGGTTACCGTGGTCATCGCTGACGACAGCGACAAGCCAGAGCGCGTTAGTGGCCCCTACGTGGAACA
  CTATCTCATGCCCTTCGGCAAGGGCTGGTTCGCAGGCCGGAACCTGGCCGTGTCTCAAGTAACCACCAAG
  TACGTGCTGTGGGTGGACGACGACTTCGTCTTCACGGCGCGGACGCGGCTGGAGAGGCTTGTGGACGTGC
  TGGAGCGGACGCCGCTGGACCTGGTGGGGGGCGCGGTGCGCGAGATCTCCGGCTTTGCCACCACTTATCG
  GCAGCTGCTGAGCGTGGAGCCCGGCGCCCCAGGCCTCGGGAACTGCCTCCGGCAAAGGCGCGGCTTCCAC
  CACGAGCTCGTCGGCTTCCCAGGCTGCGTGGTCACCGACGGCGTGGTTAACTTCTTCCTGGCGCGGACTG
  ACAAGGTGCGCGAGGTCGGTTTCGACCCCCGCCTCAGCCGCGTGGCTCATCTGGAATTCTTCTTGGATGG
  GCTTGGTTCCCTTCGGGTTGGCTCCTGCTCCGACGTCGTGGTGGATCATGCATCCAAACTGAAGCTGCCT
  TGGACATCAAGGGATGCCGGAGCAGAGACTTACGCCCGGTACCGTTACCCAGGATCACTGGACGAGAGCC
  AGATGGCCAAACACCGGCTGCTCTTCTTCAAACACCGGCTGCAGTGCATGACCTCCCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001478 unedited
  GACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCAGCCCCCACCCCATGAGACCCTCA
  GGCCGGGGCGAGAGCCCGGCACAGCCCGGACCGAAATTTTGCCGCTGCCTTAGAGCGTTA
  GACAGGATGTGGCTGGGCCGCCGGGCCCTGTGCGCTCTGGTCCTTCTGCTCGCCTGCGCC
  TCGCTGGGGCTCCTGTACGCGAGCACCCGGGACGCGCCCGGCCTCCGGCTACCTCTTGCG
  CCGTGGGCGCCCCCGCAAAGCCCCCGCAGGCCCGAGCTGCCAGATCTTGCTCCTGAGCCC
  CGCTACGCACACATCCCGGTCAGGATCAAGGAGCAAGTAGTGGGGCTGCTGGCTTGGAAA
  ACTGCAGTTGGAGCAGGNGGGGGGGGGGGCCTCCCCCTCCCCTTCAGAACAAGTCCGAGC
  TATTGACCTCACCAAGGCCTTTGACCCTGCAGAGCTGAGGGCTGCCTCTGCCACAAGAGA
  GCAGGAGTTCCAGGCCTTTCTGTCGAGGAGCCAGTCCCCAGCTGACCAGCTGCTCATAGC
  CCCTGCCAACTCCCCGCTCCAGTACCCCCTACAGGGNGGGGGGAGGTTCAGCCCCTCAGG
  AGCATCTTTGGTGCCAGGGGCTGAGCCTTCAGGCAGCTTCTGGTCAAGGAGGTATACCAN
  GTGAACCTGACTGCCTNCCTAGGCACTGGGGACTTGGCAGGGGGAAGTGACCTGGAGTTA
  CTCTCACTGGGAAGAGGGCCAGGCAGATCTCACCCTGGTCAGCCCAAGGCTGGACCAACT
  CACAGGCACTACAACTGGTCACTTACAGCAGCCGGAGCTACCAGACCACACAGAGACCAC
  AGGCCGGTCTCCAACGAGGGAAAGGAAGCGTGTTTTACTATCCGATAAGAAACCCGCCAA
  CCTTGGGTTGACCACCTGGGTTTTACCCGAGGGAGGGGAGGTTGCCAGAAAAATTAGGGT
  TTATAACAATGCCACAAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001478 unedited
  NTTAGCTTTGGACCGGCGGCCGCAATCTAGGATCGANCTTTTTTTTTTTTTTTTTTTTTT
  TGGTTGCACAGGGGACTCTGGTGCCCCTTCTTTTGTGCCCACAGCATTGCAGAGACACAA
  CTAAGAATGGCTTTCACCAACCACCAGCCCTCACCCCAGCCCCAGAGCCACAAGTTCTCT
  CTGTGGGGGTGGGGCTGGAGCAGGTACACAGAGTACTGGATCTGAAGATGCAGATGAGGG
  GCACAGTCTTGGGATTATGTGTTGGGGAACTTCCCCACCCCCTCGGTCCCAAGATGAGAG
  GACAGTGTTTCCACCTTAGGTTCTTAGAGTCCCTCTGGGCTCTTTGGCACTTGGAAAGTG
  ATCCCCCCATTTCCTGCCCCATGAGAATGGGCAGGGGGAGGACTTGGCACTGGCTGTGGG
  AGAGGTTATGGCTCCACCAGGCCTCTGGGCACTGGAAAAAGGAGGGGTGTCACCAGGACA
  ACCCCTACTGGGCATCAGGTTCTGAAAAGGAAGAGTGAGGAACTAGAGGCTCAGGGACAG
  CCAGTAGAGTGCTCACAGGTGGTGGGGGTGTTTGGTGGGAAAATTCCTGGCAGGGACAAG
  GAGGCAGGCCCAGCCTGACAGTCAGAAATCCCCAGCGGGCCATACTGGGGAGGTCATGCA
  CTGCAGCCGGTGGTTGAAGAAGAGCAGCCGGTGTTTGGCCATCTGGCTCTCGTCCAGTGA
  TCCTGGGTAACGGTACCGGCCGTAAGTCTCTGCTTCGGCATCCCTTGATGTCCAAGGCAG
  CTTCCAGTTTGGATGCATGATCCACCCCGACGTCGGGAGCAGGACCAACCCCGAAGGGAC
  CAAGCCCATCCAAGAAGAATTCCAGATGAGCCACGCTGCTGAAGGCGGGGGTCGAAACCG
  ACCCCGCACCTTGTCATTCCGGCCAAGAAAAATTACCCGCCCCCGGGGACCAACCAACTT
  GGAAAGCCACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001478
• Insert Size: 2630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001478.2, NP_001469.1
• RefSeq Size: 2885 bp
• RefSeq ORF: 1602 bp
• Locus ID: 2583
• Cytogenetics: 12q13.3
• Domains: Glycos_transf_2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• Gene Summary: 'GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).