SSH2 (NM_033389) Human Tagged ORF Clone

CAT#: RG212739

SSH2 (GFP-tagged) - Human slingshot homolog 2 (Drosophila) (SSH2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SSH2
• Synonyms: SSH-2; SSH-2L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212739 representing NM_033389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGGTCACGGTCCAGCGGTCACCTACCCCCAGCACCACCTCCAGCCCCTGCGCCTCGGAGGCAG
  ACAGTGGGGAGGAAGAATGCCGGTCACAGCCCAGGAGCATCAGCGAGAGCTTTCTAACTGTCAAAGGTGC
  TGCCCTTTTTCTACCACGGGGAAATGGCTCATCCACACCAAGAATCAGCCACAGACGGAACAAGCATGCA
  GGCGATCTCCAACAGCATCTCCAAGCAATGTTCATTTTACTCCGCCCAGAAGACAACATCAGACTGGCTG
  TAAGACTGGAAAGTACTTACCAGAATCGAACACGCTATATGGTAGTGGTTTCAACTAATGGTAGACAAGA
  CACTGAAGAAAGCATCGTCCTAGGAATGGATTTCTCCTCTAATGACAGTAGCACTTGTACCATGGGCTTA
  GTTTTGCCTCTCTGGAGCGACACGCTAATTCATTTGGATGGTGATGGTGGGTTCAGTGTATCGACGGATA
  ACAGAGTTCACATATTCAAACCTGTATCTGTGCAGGCAATGTGGTCTGCACTACAGAGCTTACACAAGGC
  TTGTGAAGTCGCCAGAGCGCATAACTACTACCCAGGCAGCCTATTTCTCACTTGGGTGAGTTATTATGAG
  AGCCATATCAACTCAGATCAATCCTCAGTCAATGAATGGAATGCAATGCAAGATGTACAGTCCCACCGGC
  CCGACTCTCCAGCTCTCTTCACCGACATACCTACTGAACGTGAACGAACAGAAAGGCTAATTAAAACCAA
  ATTAAGGGAGATCATGATGCAGAAGGATTTGGAGAATATTACATCCAAAGAGATAAGAACAGAGTTGGAA
  ATGCAAATGGTGTGCAACTTGCGGGAATTCAAGGAATTTATAGACAATGAAATGATAGTGATCCTTGGTC
  AAATGGATAGCCCTACACAGATATTTGAGCATGTGTTCCTGGGCTCAGAATGGAATGCCTCCAACTTAGA
  GGACTTACAGAACCGAGGGGTACGGTATATCTTGAATGTCACTCGAGAGATAGATAACTTCTTCCCAGGA
  GTCTTTGAGTATCATAACATTCGGGTATATGATGAAGAGGCAACGGATCTCCTGGCGTACTGGAATGACA
  CTTACAAATTCATCTCTAAAGCAAAGAAACATGGATCTAAATGCCTTGTGCACTGCAAAATGGGGGTGAG
  TCGCTCAGCCTCCACCGTGATTGCCTATGCAATGAAGGAATATGGCTGGAATCTGGACCGAGCCTATGAC
  TATGTGAAAGAAAGACGAACGGTAACCAAGCCCAACCCAAGCTTCATGAGACAACTGGAAGAGTATCAGG
  GGATCTTGCTGGCAAGCAAACAGCGGCATAACAAACTATGGAGATCTCATTCAGATAGTGACCTCTCAGA
  CCACCACGAACCCATCTGCAAACCTGGGCTAGAACTCAACAAGAAGGATATCACCACCTCAGCAGACCAG
  ATTGCTGAGGTGAAGACCATGGAGAGTCACCCACCCATACCTCCTGTCTTTGTGGAACATATGGTCCCAC
  AAGATGCAAATCAGAAAGGCCTGTGTACCAAAGAAAGAATGATCTGCTTGGAGTTTACTTCTAGGGAATT
  TCATGCTGGACAGATTGAGGATGAATTAAACTTAAATGACATCAATGGATGCTCATCAGGGTGTTGTCTG
  AATGAATCAAAATTTCCTCTTGACAATTGCCATGCATCCAAAGCCTTAATTCAGCCTGGACATGTCCCAG
  AAATGGCCAACAAGTTTCCAGACTTAACAGTGGAAGATTTGGAGACAGATGCACTGAAAGCAGACATGAA
  TGTCCACCTACTGCCTATGGAAGAATTGACATCTCCACTGAAAGACCCCCCCATGTCCCCTGATCCTGAG
  TCACCAAGCCCCCAACCCAGTTGCCAGACTGAAATCTCAGATTTCAGTACAGATCGCATTGACTTTTTTA
  GTGCCCTAGAGAAGTTTGTGGAGCTCTCCCAAGAAACCCGGTCACGATCTTTTTCCCATTCAAGGATGGA
  GGAACTGGGTGGAGGAAGGAATGAGAGCTGTCGACTGTCAGTGGTAGAAGTAGCCCCTTCCAAAGTGACA
  GCTGATGACCAGAGAAGCAGCTCTTTGAGTAATACTCCCCATGCATCAGAAGAATCTTCAATGGATGAGG
  AACAGTCAAAGGCAATTTCAGAACTGGTCAGCCCAGACATCTTCATGCAGTCTCACTCGGAAAATGCAAT
  TTCAGTCAAAGAAATTGTCACTGAAATTGAGTCCATCAGTCAAGGAGTTGGGCAGATTCAACTGAAAGGA
  GACATCTTACCCAACCCATGCCATACACCAAAGAAGAACAGCATCCATGAGCTGCTCCTTGAGAGGGCCC
  AGACTCCAGAGAACAAACCTGGACATATGGAGCAAGATGAGGACTCCTGCACAGCCCAGCCTGAACTAGC
  CAAAGACTCAGGGATGTGCAACCCAGAAGGCTGCCTAACCACACACTCATCTATAGCAGACTTGGAAGAA
  GGGGAACCAGCTGAGGGGGAACAAGAGCTCCAGGGCTCAGGGATGCACCCAGGTGCCAAGTGGTACCCTG
  GGTCTGTGAGGCGAGCCACCTTGGAGTTCGAAGAGCGCTTACGGCAGGAGCAAGAGCATCATGGTGCTGC
  CCCAACATGTACCTCATTGTCCACTCGTAAGAATTCAAAGAATGATTCTTCTGTGGCAGACCTAGCACCA
  AAAGGGAAAAGTGATGAAGCCCCCCCAGAACATTCATTTGTCCTCAAGGAACCAGAAATGAGCAAAGGCA
  AAGGGAAATACAGTGGGTCTGAGGCTGGCTCACTGTCCCATTCTGAGCAGAATGCCACTGTTCCAGCTCC
  CAGGGTGCTGGAGTTTGACCACTTGCCAGATCCTCAGGAGGGCCCAGGGTCAGATACTGGAACACAGCAG
  GAAGGAGTCCTGAAGGATCTGAGGACTGTGATTCCATACCAGGAGTCTGAAACACAAGCAGTCCCTCTTC
  CCCTTCCCAAGAGGGTAGAAATCATTGAATATACCCACATAGTTACATCACCCAATCACACTGGGCCAGG
  GAGTGAAATAGCCACCAGTGAGAAGAGCGGAGAGCAAGGGCTGAGGAAAGTGAACATGGAAAAATCTGTC
  ACTGTGCTCTGCACACTGGATGAAAATCTAAACAGGACTCTGGACCCCAACCAGGTTTCTCTGCACCCCC
  AAGTGCTACCTCTGCCTCATTCTTCCTCCCCTGAGCACAACAGACCCACTGACCATCCAACCTCCATCCT
  GAGTAGCCCTGAAGACAGAGGCAGCAGCCTGTCCACAGCCCTGGAGACAGCAGCACCTTTTGTCAGTCAT
  ACAACCCATTTACTGTCTGCCAGTTTGGATTACCTGCATCCCCAGACTATGGTTCACCTGGAGGGCTTCA
  CAGAGCAGAGCAGCACTACAGATGAGCCCTCTGCAGAACAGGTTAGCTGGGAAGAAAGTCAGGAGAGCCC
  TCTCTCCAGTGGCAGTGAGGTGCCATATAAGGACTCCCAGCTAAGTAGCGCAGACCTAAGTTTAATTAGC
  AAACTTGGTGACAACACTGGGGAGTTACAGGAGAAAATGGACCCATTGCCTGTAGCCTGTCGACTCCCAC
  ATAGCTCTAGTAGTGAAAACATAAAGAGTCTCAGCCACAGCCCCGGTGTGGTGAAGGAGCGTGCTAAAGA
  AATCGAGTCTCGAGTGGTTTTCCAGGCAGGGCTCACCAAACCATCCCAAATGAGGCGCTCAGCTTCTCTC
  GCCAAATTAGGTTACTTGGACCTCTGTAAAGACTGCTTACCAGAGAGGGAGCCTGCCTCCTGTGAATCCC
  CTCATCTCAAACTGCTTCAGCCTTTCCTCAGAACAGACTCAGGCATGCACGCGATGGAGGACCAAGAGTC
  CCTAGAAAACCCAGGTGCCCCCCACAACCCAGAGCCCACCAAGTCTTTTGTAGAACAACTCACAACAACA
  GAGTGTATTGTGCAGAGCAAGCCAGTGGAGAGGCCCCTTGTGCAGTATGCCAAAGAATTTGGTTCTAGTC
  AGCAGTATTTGCTCCCCAGGGCAGGACTTGAATTGACTAGTTCTGAAGGAGGCCTTCCCGTGCTACAGAC
  CCAGGGACTGCAGTGTGCATGCCCAGCTCCAGGGCTGGCCGTGGCACCCCGTCAGCAACACGGCAGAACT
  CACCCCCTTAGGAGACTGAAAAAGGCAAATGACAAAAAACGGACAACCAACCCCTTCTATAATACCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212739 representing NM_033389
  Red=Cloning site Green=Tags(s)
  
  MALVTVQRSPTPSTTSSPCASEADSGEEECRSQPRSISESFLTVKGAALFLPRGNGSSTPRISHRRNKHA
  GDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDSSTCTMGL
  VLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWSALQSLHKACEVARAHNYYPGSLFLTWVSYYE
  SHINSDQSSVNEWNAMQDVQSHRPDSPALFTDIPTERERTERLIKTKLREIMMQKDLENITSKEIRTELE
  MQMVCNLREFKEFIDNEMIVILGQMDSPTQIFEHVFLGSEWNASNLEDLQNRGVRYILNVTREIDNFFPG
  VFEYHNIRVYDEEATDLLAYWNDTYKFISKAKKHGSKCLVHCKMGVSRSASTVIAYAMKEYGWNLDRAYD
  YVKERRTVTKPNPSFMRQLEEYQGILLASKQRHNKLWRSHSDSDLSDHHEPICKPGLELNKKDITTSADQ
  IAEVKTMESHPPIPPVFVEHMVPQDANQKGLCTKERMICLEFTSREFHAGQIEDELNLNDINGCSSGCCL
  NESKFPLDNCHASKALIQPGHVPEMANKFPDLTVEDLETDALKADMNVHLLPMEELTSPLKDPPMSPDPE
  SPSPQPSCQTEISDFSTDRIDFFSALEKFVELSQETRSRSFSHSRMEELGGGRNESCRLSVVEVAPSKVT
  ADDQRSSSLSNTPHASEESSMDEEQSKAISELVSPDIFMQSHSENAISVKEIVTEIESISQGVGQIQLKG
  DILPNPCHTPKKNSIHELLLERAQTPENKPGHMEQDEDSCTAQPELAKDSGMCNPEGCLTTHSSIADLEE
  GEPAEGEQELQGSGMHPGAKWYPGSVRRATLEFEERLRQEQEHHGAAPTCTSLSTRKNSKNDSSVADLAP
  KGKSDEAPPEHSFVLKEPEMSKGKGKYSGSEAGSLSHSEQNATVPAPRVLEFDHLPDPQEGPGSDTGTQQ
  EGVLKDLRTVIPYQESETQAVPLPLPKRVEIIEYTHIVTSPNHTGPGSEIATSEKSGEQGLRKVNMEKSV
  TVLCTLDENLNRTLDPNQVSLHPQVLPLPHSSSPEHNRPTDHPTSILSSPEDRGSSLSTALETAAPFVSH
  TTHLLSASLDYLHPQTMVHLEGFTEQSSTTDEPSAEQVSWEESQESPLSSGSEVPYKDSQLSSADLSLIS
  KLGDNTGELQEKMDPLPVACRLPHSSSSENIKSLSHSPGVVKERAKEIESRVVFQAGLTKPSQMRRSASL
  AKLGYLDLCKDCLPEREPASCESPHLKLLQPFLRTDSGMHAMEDQESLENPGAPHNPEPTKSFVEQLTTT
  ECIVQSKPVERPLVQYAKEFGSSQQYLLPRAGLELTSSEGGLPVLQTQGLQCACPAPGLAVAPRQQHGRT
  HPLRRLKKANDKKRTTNPFYNTM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033389
• ORF Size: 4269 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033389.2, NP_203747.2
• RefSeq Size: 9166
• RefSeq ORF: 4272
• Locus ID: 85464
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a protein tyrosine phosphatase that plays a key role in the regulation of actin filaments. The encoded protein dephosphorylates and activates cofilin, which promotes actin filament depolymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]