LIPI (NM_198996) Human Tagged ORF Clone

CAT#: RG212787

LIPI (GFP-tagged) - Human lipase, member I (LIPI)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LIPI
• Synonyms: CT17; LPDL; mPA-PLA1 beta; PLA1C; PRED5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212787 representing NM_198996
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCTCAAATGTTTACATAATAACTTGTGCCAAAAATATAGTGCTCATGCTTTTCAGTTCTCACCCA
  GAAATGTCCTGTGGCTTCTAGTTGTGTGCCTGAGATCAGATAATAAAAGACCATGCCTTGAATTCTCTCA
  GCTAAGTGTAAAGGATTCCTTCAGAGATTTATTTATTCCGAGAATAGAGACCATTCTGATGATGTATACA
  AGGAACAACCTAAACTGTGCTGAGCCACTGTTTGAACAAAATAACTCACTTAATGTTAATTTCAACACAC
  AAAAGAAAACAGTCTGGCTTATTCACGGATACAGACCAGTAGGCTCCATCCCATTATGGCTTCAGAACTT
  CGTAAGGATTTTGCTGAATGAAGAAGATATGAATGTAATTGTAGTAGACTGGAGCCGGGGTGCTACAACT
  TTTATTTATAATAGAGCAGTTAAAAACACCAGAAAAGTTGCTGTGAGTTTGAGTGTGCACATTAAAAATC
  TTTTGAAGCATGGTGCATCTCTTGACAATTTTCATTTCATAGGTGTGAGCTTAGGGGCTCATATCAGTGG
  ATTTGTTGGAAAGATATTTCATGGTCAACTTGGAAGAATAACAGGTCTTGACCCTGCTGGGCCAAGGTTC
  TCCAGAAAACCACCATATAGCAGATTAGATTACACGGATGCAAAGTTTGTGGATGTCATCCATTCTGACT
  CCAATGGTTTAGGCATTCAAGAGCCCTTGGGACATATAGATTTTTATCCAAATGGAGGAAATAAACAACC
  TGGCTGTCCTAAATCAATTTTCTCAGGAATTCAATTCATTAAATGCAACCACCAGAGAGCAGTTCACTTG
  TTCATGGCATCTTTAGAAACAAACTGCAATTTTATTTCATTTCCTTGTCGTTCATACAAAGATTACAAGA
  CTAGCTTATGTGTGGACTGTGACTGTTTTAAGGAAAAATCATGTCCTCGGCTGGGTTATCAAGCCAAGCT
  ATTTAAAGGTGTTTTAAAAGAAAGGATGGAAGGAAGACCTCTTAGGACCACTGTGTTTTTGGATACAAGT
  GGTACATATCCATTCTGTACCTATTATTTTGTTCTCAGTATAATTGTTCCAGATAAAACTATGATGGATG
  GCTCGTTTTCATTTAAATTATTAAATCAGCTTGGAATGATTGAAGAGCCAAGGCTTTATGAAAAGAACAA
  ACCATTTTATAAACTTCAAGAAGTCAAGATTCTTGCTCAATTTTATAATGACTTTGTAAATATTTCAAGC
  ATTGGTTTGACATATTTCCAGAGCTCAAATCTGCAGTGTTCCACATGCACATACAAGATCCAGAGACTCA
  TGTTAAAATCACTTACATACCCAGAAAGACCACCACTTTGCAGGTATAATATTGTACTTAAAGACAGAGA
  GGAAGTGTTTCTTAATCCAAACACATGTACACCAAAGAACACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212787 representing NM_198996
  Red=Cloning site Green=Tags(s)
  
  MLLKCLHNNLCQKYSAHAFQFSPRNVLWLLVVCLRSDNKRPCLEFSQLSVKDSFRDLFIPRIETILMMYT
  RNNLNCAEPLFEQNNSLNVNFNTQKKTVWLIHGYRPVGSIPLWLQNFVRILLNEEDMNVIVVDWSRGATT
  FIYNRAVKNTRKVAVSLSVHIKNLLKHGASLDNFHFIGVSLGAHISGFVGKIFHGQLGRITGLDPAGPRF
  SRKPPYSRLDYTDAKFVDVIHSDSNGLGIQEPLGHIDFYPNGGNKQPGCPKSIFSGIQFIKCNHQRAVHL
  FMASLETNCNFISFPCRSYKDYKTSLCVDCDCFKEKSCPRLGYQAKLFKGVLKERMEGRPLRTTVFLDTS
  GTYPFCTYYFVLSIIVPDKTMMDGSFSFKLLNQLGMIEEPRLYEKNKPFYKLQEVKILAQFYNDFVNISS
  IGLTYFQSSNLQCSTCTYKIQRLMLKSLTYPERPPLCRYNIVLKDREEVFLNPNTCTPKNT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198996
• ORF Size: 1443 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_198996.2, NP_945347.1
• RefSeq Size: 1636
• RefSeq ORF: 1446
• Locus ID: 149998
• Protein Families: Secreted Protein
• Gene Summary: The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. This gene is also expressed at high levels in Ewing family tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]