RICTOR (NM_152756) Human Tagged ORF Clone

CAT#: RG212982

RICTOR (GFP-tagged) - Human RPTOR independent companion of MTOR, complex 2 (RICTOR)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RICTOR
• Synonyms: AVO3; hAVO3; PIA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212982 representing NM_152756
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGATCGGCCGCGGCCGCTCTCTGAAGAACCTCCGAGTACGAGGGCGGAATGACAGCGGCGAGG
  AGAACGTCCCGCTGGATCTGACCCGAGAACCTTCTGATAACTTAAGAGAGATTCTCCAAAATGTGGCCAG
  ATTGCAGGGAGTATCAAATATGAGAAAGCTAGGCCATCTGAATAACTTTACTAAGCTTCTTTGTGATATT
  GGCCACAGTGAAGAAAAACTGGGCTTTCACTATGAGGATATCATAATTTGTTTGCGGTTAGCTTTATTAA
  ATGAAGCAAAAGAAGTGCGAGCAGCAGGGCTACGAGCGCTTCGATATCTCATCCAAGACTCCAGTATTCT
  CCAGAAGGTGCTAAAATTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATACAACAGAGCAACGAG
  GTAGAGAGGACACAAGCACTTCGATTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTGTTTCCTAGTT
  CTGTGACCAACTCATTAATTGCAGTTGGAAATGATGGACTTCAAGAAAGAGACAGAATGGTCCGAGCATG
  CATTGCCATTATCTGTGAACTAGCACTTCAGAATCCAGAGGTGGTGGCCCTTCGAGGTGGACTAAACACC
  ATCTTGAAAAATGTGATCGATTGCCAATTAAGTCGAATAAATGAGGCCCTAATTACTACAATTTTGCACC
  TTCTTAATCATCCAAAGACTCGACAGTATGTGCGAGCTGATGTAGAATTAGAGAGAATTTTAGCACCCTA
  TACTGATTTTCACTACAGACATAGTCCAGATACAGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGA
  TTTCTAGCCAGTAAAATGGGAATCATAGCAACATTCCGATCATGGGCAGGTATTATTAATTTATGTAAAC
  CTGGAAATTCTGGGATCCAGTCTCTAATAGGAGTACTTTGCATACCAAATATGGAAATAAGGCGAGGTCT
  ACTTGAAGTGCTTTATGATATATTTCGTCTTCCTCTACCTGTTGTGACTGAGGAGTTCATAGAAGCACTA
  CTCAGTGTAGATCCAGGGAGGTTCCAAGACAGTTGGAGGCTTTCAGATGGCTTTGTGGCAGCTGAGGCAA
  AAACTATTCTTCCTCATCGTGCCAGATCCAGGCCAGACCTCATGGATAATTATTTGGCACTGATACTCTC
  TGCATTTATTCGTAATGGACTTTTAGAGGGTCTAGTTGAAGTGATAACAAACAGTGATGATCATATCTCA
  GTTAGAGCTACCATCCTTTTAGGAGAGCTTTTACATATGGCAAACACAATTCTTCCTCATTCACATAGCC
  ATCATTTACACTGCTTGCCAACCCTAATGAATATGGCTGCATCCTTTGATATCCCCAAGGAAAAGAGACT
  GCGAGCCAGTGCAGCCTTGAACTGTTTAAAACGCTTCCATGAAATGAAGAAACGAGGACCTAAGCCTTAT
  AGTCTTCATTTAGACCACATTATTCAGAAAGCAATTGCAACACACCAGAAACGGGATCAGTATCTCCGAG
  TTCAGAAAGATATATTTATCCTTAAGGATACAGAGGAAGCTCTTTTAATTAACCTTAGAGATAGCCAAGT
  CCTTCAACATAAAGAGAATCTTGAATGGAATTGGAATCTTATAGGGACCATTCTTAAGTGGCCAAATGTA
  AATCTAAGAAACTATAAAGATGAACAGTTACACAGGTTTGTACGAAGACTACTTTATTTTTACAAGCCCA
  GCAGTAAATTATATGCCAACCTGGATCTGGATTTTGCCAAGGCCAAACAGCTCACGGTTGTAGGTTGCCA
  GTTTACAGAATTTCTTCTTGAATCTGAAGAGGATGGGCAAGGCTACTTAGAAGATCTAGTAAAGGATATT
  GTTCAGTGGCTCAATGCTTCATCTGGAATGAAACCCGAAAGAAGTCTTCAAAATAATGGTTTATTGACCA
  CCCTTAGTCAACACTACTTTTTATTTATTGGAACACTTTCTTGCCACCCTCATGGAGTTAAAATGCTGGA
  AAAATGCAGTGTATTTCAGTGTCTCCTTAATCTTTGCTCCTTGAAAAACCAAGATCACTTGCTAAAACTT
  ACTGTTTCTAGCTTGGACTATAGCAGAGATGGATTGGCTAGAGTCATCCTTTCCAAAATTTTAACTGCAG
  CTACTGATGCCTGCAGACTCTATGCAACAAAACATTTAAGGGTATTATTGAGAGCTAATGTTGAATTCTT
  TAATAATTGGGGAATTGAGTTGTTAGTGACCCAGCTACATGATAAAAACAAAACGATTTCCTCTGAAGCT
  CTTGATATCCTCGATGAAGCATGTGAAGACAAGGCCAATCTTCATGCTCTCATTCAGATGAAACCAGCGT
  TATCCCACCTTGGAGACAAGGGTTTGCTTCTCCTGCTGAGATTTCTCTCCATTCCAAAAGGATTTTCCTA
  TCTGAATGAAAGAGGTTATGTAGCAAAACAATTGGAAAAGTGGCACAGGGAATACAACTCCAAATATGTT
  GACTTGATTGAGGAACAACTCAATGAAGCACTTACTACTTACCGGAAGCCTGTTGATGGTGATAACTATG
  TTCGTCGGAGTAACCAAAGATTACAGCGTCCTCACGTCTACCTGCCTATACACCTTTATGGACAACTAGT
  ACACCATAAAACAGGCTGCCATTTGTTGGAAGTACAGAATATTATTACAGAACTCTGTCGTAATGTTCGT
  ACACCAGATTTGGATAAGTGGGAAGAAATTAAAAAACTGAAAGCATCTCTTTGGGCCTTGGGAAATATCG
  GCTCATCAAATTGGGGTCTCAATTTGCTACAGGAAGAAAACGTGATTCCAGATATACTAAAACTTGCAAA
  ACAGTGTGAAGTTCTTTCCATCAGAGGGACCTGTGTATATGTACTTGGGCTCATAGCTAAAACCAAACAA
  GGCTGTGATATTCTAAAATGTCACAACTGGGATGCTGTGAGGCATAGTCGCAAACATCTGTGGCCAGTGG
  TTCCAGATGATGTGGAACAACTCTGTAATGAACTTTCATCTATCCCAAGCACTCTAAGTTTGAACTCGGA
  GTCAACCAGCTCTAGACATAATAGTGAAAGTGAATCTGTGCCATCGAGTATGTTCATATTGGAGGATGAC
  CGGTTTGGCAGCAGCTCTACTAGTACATTTTTCCTTGATATCAATGAAGATACAGAGCCAACATTTTATG
  ACCGATCTGGACCCATAAAGGATAAAAATTCATTCCCTTTCTTTGCTTCTAGTAAACTTGTGAAGAATCG
  TATCTTAAATTCGCTTACTTTGCCTAACAAAAAACATCGTAGTAGCAGTGATCCAAAAGGAGGGAAATTA
  TCATCTGAAAGTAAGACAAGCAACAGGCGAATCAGAACACTTACGGAGCCCAGTGTTGATTTTAATCATA
  GTGATGATTTTACACCCATATCCACTGTACAGAAAACATTACAATTAGAGACTTCATTTATGGGGAATAA
  GCACATTGAAGACACTGGTAGTACACCAAGCATTGGAGAAAATGACTTAAAATTCACCAAGAATTTTGGT
  ACAGAGAATCACAGAGAAAATACAAGCCGAGAGAGGTTAGTAGTAGAAAGTTCAACGAGCTCACATATGA
  AGATACGTAGCCAAAGTTTCAATACAGACACTACAACAAGTGGCATAAGTTCAATGAGCTCAAGTCCTTC
  ACGAGAGACAGTAGGTGTAGATGCTACAACTATGGACACAGACTGTGGAAGCATGAGTACTGTGGTAAGT
  ACTAAAACTATTAAGACAAGCCACTATTTGACGCCACAGTCTAACCATCTGTCTCTCTCCAAATCAAATT
  CGGTGTCCCTGGTGCCTCCAGGTTCTTCTCATACGCTTCCTAGAAGAGCACAGTCCCTTAAAGCACCCTC
  TATTGCTACAATTAAAAGTCTAGCAGATTGTAACTTTAGTTACACAAGTTCTAGAGATGCTTTTGGCTAT
  GCTACACTGAAAAGACTACAGCAACAAAGAATGCATCCATCCTTATCTCACTCTGAAGCTTTGGCATCTC
  CAGCAAAAGATGTGCTATTTACTGATACCATCACCATGAAGGCCAACAGTTTTGAGTCCAGATTAACACC
  AAGCAGGTTCATGAAAGCCTTAAGTTATGCATCATTAGATAAAGAAGATTTATTGAGTCCTATTAATCAA
  AATACCCTGCAACGATCTTCCTCAGTGCGGTCCATGGTGTCCAGTGCCACATATGGGGGTTCAGATGATT
  ACATTGGTCTTGCTCTCCCGGTGGATATAAATGATATATTCCAGGTAAAGGATATTCCCTATTTTCAGAC
  AAAAAACATACCACCACATGATGATCGAGGTGCAAGAGCATTTGCCCATGATGCAGGAGGTCTTCCATCT
  GGAACTGGAGGTCTTGTAAAAAATTCTTTTCACTTGCTACGACAGCAGATGAGTCTTACGGAAATAATGA
  ATTCAATCCATTCAGATGCCTCTCTGTTTTTAGAAAGTACAGAAGACACTGGACTACAGGAACATACAGA
  TGATAACTGCCTTTATTGTGTCTGTATTGAAATTCTGGGTTTCCAGCCCAGCAACCAACTGAGTGCAATA
  TGTAGTCATTCAGACTTTCAAGATATTCCATATTCTGATTGGTGTGAGCAGACTATCCATAATCCTTTAG
  AAGTGGTTCCCTCTAAGTTTTCGGGGATTTCTGGATGCAGTGATGGGGTGTCTCAAGAAGGCTCAGCTAG
  CAGCACCAAAAGCACAGAATTGTTACTAGGTGTTAAAACAATTCCAGATGATACACCAATGTGCCGTATA
  CTCCTTCGCAAAGAAGTTCTAAGATTAGTCATTAATTTGAGTAGTTCAGTTTCAACTAAATGTCATGAGA
  CTGGGCTTTTAACAATTAAGGAGAAGTATCCTCAAACATTTGATGACATATGCCTTTACTCTGAGGTTTC
  CCATTTGCTGTCACACTGCACATTCAGACTTCCGTGTCGGAGGTTCATACAAGAATTATTTCAAGATGTA
  CAGTTTCTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAACACCACCAAAGCAACCTATAGTTGATA
  CATCTGCTGAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212982 representing NM_152756
  Red=Cloning site Green=Tags(s)
  
  MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDI
  GHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNE
  VERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNT
  ILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREAR
  FLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEAL
  LSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHIS
  VRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPY
  SLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLEWNWNLIGTILKWPNV
  NLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDI
  VQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKL
  TVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEA
  LDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYV
  DLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVR
  TPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQ
  GCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDD
  RFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKL
  SSESKTSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFG
  TENHRENTSRERLVVESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVS
  TKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGY
  ATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQ
  NTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPS
  GTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQLSAI
  CSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLLGVKTIPDDTPMCRI
  LLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDV
  QFLQMHEEAEAVLATPPKQPIVDTSAES
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152756
• ORF Size: 5124 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152756.3, NP_689969.2
• RefSeq Size: 9557
• RefSeq ORF: 5127
• Locus ID: 253260
• Protein Pathways: mTOR signaling pathway
• Gene Summary: RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]). [supplied by OMIM, Mar 2008]