TAF6 (NM_005641) Human Tagged ORF Clone

CAT#: RG213036

TAF6 (GFP-tagged) - Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TAF6
• Synonyms: ALYUS; MGC:8964; TAF(II)70; TAF(II)80; TAF2E; TAFII-70; TAFII-80; TAFII70; TAFII80; TAFII85
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213036 representing NM_005641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGAGAAGAAGCTGAAGCTTAGCAACACTGTGCTGCCCTCGGAGTCCATGAAGGTGGTGGCTG
  AATCCATGGGCATCGCCCAGATTCAGGAGGAGACCTGCCAGCTGCTAACGGATGAGGTCAGCTACCGCAT
  CAAAGAGATCGCACAGGATGCCTTGAAGTTCATGCACATGGGGAAGCGGCAGAAGCTCACCACCAGTGAC
  ATTGACTACGCCTTGAAGCTAAAGAATGTCGAGCCACTCTATGGCTTCCACGCCCAGGAGTTCATTCCTT
  TCCGCTTCGCCTCTGGTGGGGGCCGGGAGCTTTACTTCTATGAGGAGAAGGAGGTTGATCTGAGCGACAT
  CATCAATACCCCTCTGCCCCGGGTGCCCCTGGACGTCTGCCTCAAAGCTCATTGGCTGAGCATCGAGGGC
  TGCCAGCCAGCTATCCCCGAGAACCCGCCCCCAGCTCCCAAAGAGCAACAGAAGGCTGAAGCCACAGAAC
  CCCTGAAGTCAGCCAAGCCAGGCCAGGAGGAAGACGGACCCCTGAAGGGCAAAGGTCAAGGGGCCACCAC
  AGCCGACGGCAAAGGGAAAGAGAAGAAGGCGCCGCCCTTGCTGGAGGGGGCCCCCTTGCGACTGAAGCCC
  CGGAGCATCCACGAGTTGTCTGTGGAGCAGCAGCTCTACTACAAGGAGATCACCGAGGCCTGCGTGGGCT
  CCTGCGAGGCCAAGAGGGCGGAAGCCCTGCAAAGCATTGCCACGGACCCTGGACTGTATCAGATGCTGCC
  ACGGTTCAGTACCTTTATCTCGGAGGGGGTCCGTGTGAACGTGGTTCAGAACAACCTGGCCCTACTCATC
  TACCTGATGCGTATGGTGAAAGCGCTGATGGACAACCCCACGCTCTATCTAGAAAAATACGTCCATGAGC
  TGATTCCAGCTGTGATGACCTGCATCGTGAGCAGACAGTTGTGCCTGCGACCAGATGTGGACAATCACTG
  GGCACTCCGAGACTTTGCTGCCCGCCTGGTGGCCCAGATCTGCAAGCATTTTAGCACAACCACTAACAAC
  ATCCAGTCCCGGATCACCAAGACCTTCACCAAGAGCTGGGTGGACGAGAAGACGCCCTGGACGACTCGTT
  ATGGCTCCATCGCAGGCTTGGCTGAGCTGGGACACGATGTTATCAAGACTCTGATTCTGCCCCGGCTGCA
  GCAGGAAGGGGAGCGGATCCGCAGTGTGCTGGACGGCCCTGTGCTGAGCAACATTGACCGGATTGGAGCA
  GACCATGTGCAGAGCCTCCTGCTGAAACACTGTGCTCCTGTTCTGGCAAAGCTGCGCCCACCGCCTGACA
  ATCAGGACGCCTATCGGGCAGAATTCGGGTCCCTTGGGCCCCTCCTCTGCTCCCAGGTGGTCAAGGCTCG
  GGCCCAGGCTGCTCTGCAGGCTCAGCAGGTCAACAGGACCACTCTGACCATCACGCAGCCCCGGCCCACG
  CTGACCCTCTCGCAGGCCCCACAGCCTGGCCCTCGCACCCCTGGCTTGCTGAAGGTTCCTGGCTCCATCG
  CACTTCCTGTCCAGACACTGGTGTCTGCACGAGCGGCTGCCCCACCACAGCCTTCCCCTCCTCCAACCAA
  GTTTATTGTAATGTCATCGTCCTCCAGCGCCCCATCCACCCAGCAGGTCCTGTCCCTCAGCACCTCGGCC
  CCCGGCTCAGGTTCCACCACCACTTCGCCCGTCACCACCACCGTCCCCAGCGTGCAGCCCATCGTCAAGT
  TGGTCTCCACCGCCACCACCGCACCCCCCAGCACTGCTCCCTCTGGTCCTGGGAGTGTCCAGAAGTACAT
  CGTGGTCTCACTTCCCCCAACAGGGGAGGGCAAAGGAGGCCCCACCTCCCATCCTTCTCCAGTTCCTCCC
  CCGGCATCGTCCCCGTCCCCACTCAGCGGCAGTGCCCTTTGTGGGGGGAAGCAGGAGGCTGGGGACAGTC
  CCCCTCCAGCTCCAGGGACTCCAAAAGCCAATGGCTCCCAGCCCAACTCCGGCTCCCCTCAGCCTGCTCC
  G
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213036 representing NM_005641
  Red=Cloning site Green=Tags(s)
  
  MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHMGKRQKLTTSD
  IDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINTPLPRVPLDVCLKAHWLSIEG
  CQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKGKGQGATTADGKGKEKKAPPLLEGAPLRLKP
  RSIHELSVEQQLYYKEITEACVGSCEAKRAEALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLI
  YLMRMVKALMDNPTLYLEKYVHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNN
  IQSRITKTFTKSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGA
  DHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRTTLTITQPRPT
  LTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIVMSSSSSAPSTQQVLSLSTSA
  PGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPSGPGSVQKYIVVSLPPTGEGKGGPTSHPSPVPP
  PASSPSPLSGSALCGGKQEAGDSPPPAPGTPKANGSQPNSGSPQPAP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005641
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005641.2, NP_005632.1
• RefSeq Size: 2737 bp
• RefSeq ORF: 2034 bp
• Locus ID: 6878
• Cytogenetics: 7q22.1
• Domains: TAF
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]'