TAF6 (NM_005641) Human Untagged Clone

CAT#: SC111695

TAF6 (untagged)-Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF6
• Synonyms: ALYUS; MGC:8964; TAF(II)70; TAF(II)80; TAF2E; TAFII-70; TAFII-80; TAFII70; TAFII80; TAFII85
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005641, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGAGGAGAAGAAGCTGAAGCTTAGCAACACTGTGCTGCCCTCGGAGTCCATGAAGGTGGTGGCTG
  AATCCATGGGCATCGCCCAGATTCAGGAGGAGACCTGCCAGCTGCTAACGGATGAGGTCAGCTACCGCAT
  CAAAGAGATCGCACAGGATGCCTTGAAGTTCATGCACATGGGGAAGCGGCAGAAGCTCACCACCAGTGAC
  ATTGACTACGCCTTGAAGCTAAAGAATGTCGAGCCACTCTATGGCTTCCACGCCCAGGAGTTCATTCCTT
  TCCGCTTCGCCTCTGGTGGGGGCCGGGAGCTTTACTTCTATGAGGAGAAGGAGGTTGATCTGAGCGACAT
  CATCAATACCCCTCTGCCCCGGGTGCCCCTGGACGTCTGCCTCAAAGCTCATTGGCTGAGCATCGAGGGC
  TGCCAGCCAGCTATCCCCGAGAACCCGCCCCCAGCTCCCAAAGAGCAACAGAAGGCTGAAGCCACAGAAC
  CCCTGAAGTCAGCCAAGCCAGGCCAGGAGGAAGACGGACCCCTGAAGGGCAAAGGTCAAGGGGCCACCAC
  AGCCGACGGCAAAGGGAAAGAGAAGAAGGCGCCGCCCTTGCTGGAGGGGGCCCCCTTGCGACTGAAGCCC
  CGGAGCATCCACGAGTTGTCTGTGGAGCAGCAGCTCTACTACAAGGAGATCACCGAGGCCTGCGTGGGCT
  CCTGCGAGGCCAAGAGGGCGGAAGCCCTGCAAAGCATTGCCACGGACCCTGGACTGTATCAGATGCTGCC
  ACGGTTCAGTACCTTTATCTCGGAGGGGGTCCGTGTGAACGTGGTTCAGAACAACCTGGCCCTACTCATC
  TACCTGATGCGTATGGTGAAAGCGCTGATGGACAACCCCACGCTCTATCTAGAAAAATACGTCCATGAGC
  TGATTCCAGCTGTGATGACCTGCATCGTGAGCAGACAGTTGTGCCTGCGACCAGATGTGGACAATCACTG
  GGCACTCCGAGACTTTGCTGCCCGCCTGGTGGCCCAGATCTGCAAGCATTTTAGCACAACCACTAACAAC
  ATCCAGTCCCGGATCACCAAGACCTTCACCAAGAGCTGGGTGGACGAGAAGACGCCCTGGACGACTCGTT
  ATGGCTCCATCGCAGGCTTGGCTGAGCTGGGACACGATGTTATCAAGACTCTGATTCTGCCCCGGCTGCA
  GCAGGAAGGGGAGCGGATCCGCAGTGTGCTGGACGGCCCTGTGCTGAGCAACATTGACCGGATTGGAGCA
  GACCATGTGCAGAGCCTCCTGCTGAAACACTGTGCTCCTGTTCTGGCAAAGCTGCGCCCACCGCCTGACA
  ATCAGGACGCCTATCGGGCAGAATTCGGGTCCCTTGGGCCCCTCCTCTGCTCCCAGGTGGTCAAGGCTCG
  GGCCCAGGCTGCTCTGCAGGCTCAGCAGGTCAACAGGACCACTCTGACCATCACGCAGCCCCGGCCCACG
  CTGACCCTCTCGCAGGCCCCACAGCCTGGCCCTCGCACCCCTGGCTTGCTGAAGGTTCCTGGCTCCATCG
  CACTTCCTGTCCAGACACTGGTGTCTGCACGAGCGGCTGCCCCACCACAGCCTTCCCCTCCTCCAACCAA
  GTTTATTGTAATGTCATCGTCCTCCAGCGCCCCATCCACCCAGCAGGTCCTGTCCCTCAGCACCTCGGCC
  CCCGGCTCAGGTTCCACCACCACTTCGCCCGTCACCACCACCGTCCCCAGCGTGCAGCCCATCGTCAAGT
  TGGTCTCCACCGCCACCACCGCACCCCCCAGCACTGCTCCCTCTGGTCCTGGGAGTGTCCAGAAGTACAT
  CGTGGTCTCACTTCCCCCAACAGGGGAGGGCAAAGGAGGCCCCACCTCCCATCCTTCTCCAGTTCCTCCC
  CCGGCATCGTCCCCGTCCCCACTCAGCGGCAGTGCCCTTTGTGGGGGGAAGCAGGAGGCTGGGGACAGTC
  CCCCTCCAGCTCCAGGGACTCCAAAAGCCAATGGCTCCCAGCCCAACTCCGGCTCCCCTCAGCCTGCTCC
  GTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005641 unedited
  NNTGTTTGCGCATTTATGTATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGC
  GCCAAGCGCCGGGTGAGCAGCGTCTCGGCTGCCGCTAGAGTTTTCCTGCTCCCCGCGCTC
  GGCTGGCGGGGGCGGGTCTGAGTGGTACCCCGGAGGAGACCCTTTGAAGGTCCCTTGTGG
  GGACTGGAAAGAGGACGGGTCTCCGTCTCTCCACCGGGGGCTTCATCCTTCCAGGGAGGA
  GAAGAGGGACTCCAGAATGGCTGAGGAGAAGAAGCTGAAGCTTAGCAACACTGTGCTGCC
  CTCGGAGTCCATGAAGGTGGTGGCTGAATCCATGGGCATCGCCCAGATTCAGGAGGAGAC
  CTGCCAGCTGCTAACGGATGAGGTCAGCTACCGCATCAAAGAGATCGCACAGGATGCCTT
  GAAGTTCATGCACATGGGGAAGCGGCAGAAGCTCACCACCAGTGACATTGACTACGCCTT
  GAAGCTAAAGAATGTCGAGCCACTCTATGGCTTCCACGCCCAGGAGTTCATTCCTTTCCG
  CTTCGCCTCTGGTGGGGGCCGGGAGCTTTACTTCTATGAGGAGAAGGAGGTTGATCTGAG
  CGACATCATCAATACCCCTCTGCCCCGGGTGCCCCTGGACGTCTGCCTCAAAGCTCATTG
  GCTGAGCATCGAGGGCTGCCAGCCAGCTATCCCCGAGAACCCGCCCCCAGCTCCCAAAGA
  GCAACAGAAGGCTGAAGCCACAGAACCCCTGNAGTCAGCCAAGCCAGGCCAGGNAGAAGA
  CGGACCCCTNGAGGGCANAGGTCAAGGNGCCACCACAGCCGACGGCANAGGGAAAGAGAA
  GAANGCGCCGCCTTGCTGTNNAGGGCCCCCTTTGCGACTGAGCCCCGNACATNCAGAGNT
  GTCTGTGGAGCAGCAGCTCTACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005641 unedited
  CGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACAAACCAAACTTTTATTCTGAGAA
  ACTGGCTGTACAATATCTAAAAAGAAAGTGACATGAAGGAAGCAATCTACAACTTCCTTC
  CGCTTATCGTGCATGCATGTGTGTACGTGCACGTGTGTACGTGTCTGCATGTGTGGGAAT
  CCGGGGGCTGGCAGGTGGAGCATCACGGAGCAGGCTGAGGGGAGCCGGAGTTGGGCTGGG
  AGCCATTGGCTTTTGGAGTCCCTGGAGCTGGAGGGGGACTGTCCCCAGCCTCCTGCTTCC
  CCCCACAAAGGGCACTGCCGCTGAGTGGGGACGGGGACGATGCCGGGGGAGGAACTGGAG
  AAGGATGGGAGGTGGGGCCTCCTTTGCCCTCCCCTGTTGGGGGAAGTGAGACCACGATGT
  ACTTCTGGACACTCCCAGGACCAGAGGGATCATTGCTGGGGGGTGCGGTGGTGGCGGTGG
  ATACCAACTTGACGATGGGCTGCACGCTGGGGACGGTGGTGGTGACGGGCGAAGTGGTGG
  TGGAACCTGAGCCGGAGGCCGAGGTGCTGAGGGACAGGACCTGCTGGGTGGATGGGGCGC
  TGGAGGACGATGACATTACAATAACTTTTGTCTGGAAAGATGGGATAGCTTGTGGTGGGG
  CATCCGCTCGTGCAGACACCATTGTCTGGACAGTATGTGCGATTGGACCCAGGAACCTTC
  ATCATGCCATGGGTTGCGATGCCCAAGCTGTGGGGCCTGCCATAGGTTCATCGTGGGCCC
  GGGCTGCGTGATGGTCATAGTGGTCCTGTTGCCCTGCTGAGCCTGCATAACAACCTGGGC
  CCGAACCTTGACCACCTGGGAACACAAGAAGGGGCCCAAGGTGACCCGAATTTTGCCCGA
  TAGGCCTCCTGTTGTTTATGCGGTGGCGCCATTCTTCCAAAAAAAGAAACAAGGGTCTTA
  ACACGAGTCTCTCAAATGGTCTGCTCTAATCCGTCAAGGTCGTAACCAACGGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005641
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005641.2, NP_005632.1
• RefSeq Size: 2787 bp
• RefSeq ORF: 2787 bp
• Locus ID: 6878
• Cytogenetics: 7q22.1
• Domains: TAF
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) encodes isoform alpha. Both variants 1 and 2 encode the same isoform.