FBXO22 (NM_012170) Human Tagged ORF Clone

CAT#: RG213115

  • TrueORF®

FBXO22 (GFP-tagged) - Human F-box protein 22 (FBXO22), transcript variant 2


  "NM_012170" in other vectors (4)

Reconstitution Protocol

USD 460.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol FBXO22
Synonyms FBX22; FISTC1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG213115 representing NM_012170
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGGTAGGCTGCTGCGGCGAGTGCCGCGGCTCCTCCGTAGACCCGCGGAGCACCTTCGTGTTGA
GTAACCTGGCGGAGGTGGTGGAGCGTGTGCTCACCTTCCTGCCCGCCAAGGCGTTGCTGCGGGTGGCCTG
CGTGTGCCGCTTATGGAGGGAGTGTGTGCGCAGAGTATTGCGGACCCATCGGAGCGTAACCTGGATCTCC
GCAGGCCTGGCGGAGGCCGGCCACCTGGAGGGGCATTGCTTGGTTCGCGTGGTAGCAGAGGAGCTTGAGA
ATGTTCGCATCTTACCACATACAGTTCTTTACATGGCTGATTCAGAAACTTTCATTAGTCTGGAAGAGTG
TCGTGGCCATAAGAGAGCAAGGAAAAGAACTAGTATGGAAACAGCACTTGCCCTTGAGAAGCTATTCCCC
AAACAATGCCAAGTCCTTGGGATTGTGACCCCAGGAATTGTAGTGACTCCAATGGGATCAGGTAGCAATC
GACCTCAGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTATTATTCCCTCAAATTGAAGGAATAAAAAT
ACAACCCTTTCATTTTATTAAGGATCCAAAGAATTTAACATTAGAAAGACATCAACTCACTGAAGTAGGT
CTTTTAGATAACCCTGAACTTCGTGTGGTCCTTGTCTTTGGTTATAATTGCTGTAAGGTGGGAGCCAGTA
ATTATCTGCAGCAAGTAGTCAGCACTTTCAGTGATATGAATATCATCTTGGCTGGAGGCCAGGTGGACAA
CCTGTCATCACTGACTTCTGAAAAGTATGTCTTGTGTGCTTCTGATTTCGTCTGTGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG213115 representing NM_012170
Red=Cloning site Green=Tags(s)

MEPVGCCGECRGSSVDPRSTFVLSNLAEVVERVLTFLPAKALLRVACVCRLWRECVRRVLRTHRSVTWIS
AGLAEAGHLEGHCLVRVVAEELENVRILPHTVLYMADSETFISLEECRGHKRARKRTSMETALALEKLFP
KQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESGFALLFPQIEGIKIQPFHFIKDPKNLTLERHQLTEVG
LLDNPELRVVLVFGYNCCKVGASNYLQQVVSTFSDMNIILAGGQVDNLSSLTSEKYVLCASDFVCE

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012170
ORF Size 828 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_012170.2, NP_036302.1
RefSeq Size 1822
RefSeq ORF 831
Locus ID 26263
Domains F-box
Protein Families Druggable Genome
Gene Summary This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and, as a transcriptional target of the tumor protein p53, is thought to be involved in degradation of specific proteins in response to p53 induction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

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