TIAM2 (NM_001010927) Human Tagged ORF Clone

CAT#: RG213556

TIAM2 (GFP-tagged) - Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TIAM2
• Synonyms: STEF; TIAM-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213556 representing NM_001010927
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGACCGCGGGAGAATCAGGATCCTCCTCCGAGGCCTCTGGCCCGCCACCTGTCTGATGCAGACC
  GCCTCCGCAAAGTCATCCAGGAGCTTGTGGACACAGAGAAGTCCTACGTGAAGGATTTGAGCTGCCTCTT
  TGAATTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTTGGA
  AGTTTGCCAGAGATGCTTGAGTTTCAGAAGGTGTTTCTGGAGACCCTGGAGGATGGGATTTCAGCATCAT
  CTGACTTTAACACCCTAGAAACCCCCTCACAGTTTAGAAAATTACTGTTTTCCCTTGGAGGCTCTTTCCT
  TTATTACGCGGACCACTTTAAACTGTACAGTGGATTCTGTGCTAACCATATCAAAGTACAGAAGGTTCTG
  GAGCGAGCTAAAACTGACAAAGCCTTCAAGGCTTTTCTGGACGCCCGGAACCCCACCAAGCAGCATTCCT
  CCACGCTGGAGTCCTACCTCATCAAGCCGGTTCAGAGAGTGCTCAAGTACCCGCTGCTGCTCAAGGAGCT
  GGTGTCCCTGACGGACCAGGAGAGCGAGGAGCACTACCACCTGACGGAAGCACTAAAGGCAATGGAGAAA
  GTAGCGAGCCACATCAATGAGATGCAGAAGATCTATGAGGATTATGGGACCGTGTTTGACCAGCTAGTAG
  CTGAGCAGAGCGGAACAGAGAAGGAGGTAACAGAACTTTCGATGGGAGAGCTTCTGATGCACTCTACGGT
  TTCCTGGTTGAATCCATTTCTGTCTCTAGGAAAAGCTAGAAAGGACCTTGAGCTCACAGTATTTGTTTTT
  AAGAGAGCCGTCATACTGGTTTATAAAGAAAACTGCAAACTGAAAAAGAAATTGCCCTCGAATTCCCGGC
  CTGCACACAACTCTACTGACTTGGACCCATTTAAATTCCGCTGGTTGATCCCCATCTCCGCGCTTCAAGT
  CAGACTGGGGAATCCAGCAGGGACAGAAAATAATTCCATATGGGAACTGATCCATACGAAGTCAGAAATA
  GAAGGACGGCCAGAAACCATCTTTCAGTTGTGTTGCAGTGACAGTGAAAGCAAAACCAACATTGTTAAGG
  TGATTCGTTCTATTCTGAGGGAGAACTTCAGGCGTCACATAAAGTGTGAATTACCACTGGAGAAAACGTG
  TAAGGATCGCCTGGTACCTCTTAAGAACCGAGTTCCTGTTTCGGCCAAATTAGCTTCATCCAGGTCTTTA
  AAAGTCCTGAAGAATTCCTCCAGCAACGAGTGGACCGGTGAGACTGGCAAGGGAACCTTGCTGGACTCTG
  ACGAGGGCAGCTTGAGCAGCGGCACCCAGAGCAGCGGCTGCCCCACGGCTGAGGGCAGGCAGGACTCCAA
  GAGCACTTCTCCCGGGAAATACCCACACCCCGGCTTGGCAGATTTTGCCGACAATCTCATCAAAGAGAGT
  GACATCCTGAGCGATGAAGATGATGACCACCGTCAGACTGTGAAGCAGGGCAGCCCTACTAAAGACATCG
  AAATTCAGTTCCAGAGACTGAGGATTTCCGAGGACCCAGACGTTCACCCCGAGGCTGAGCAGCAGCCTGG
  CCCGGAGTCGGGTGAGGGTCAGAAAGGAGGAGAGCAGCCCAAACTGGTCCGGGGGCACTTCTGCCCCATT
  AAACGAAAAGCCAACAGCACCAAGAGGGACAGAGGAACTTTGCTCAAGGCGCAGATCCGTCACCAGTCCC
  TTGACAGTCAGTCTGAAAATGCCACCATCGACCTAAATTCTGTTCTAGAGCGAGAATTCAGTGTCCAGAG
  TTTAACATCTGTTGTCAGTGAGGAGTGTTTTTATGAAACAGAGAGCCACGGAAAATCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213556 representing NM_001010927
  Red=Cloning site Green=Tags(s)
  
  MEGPRENQDPPPRPLARHLSDADRLRKVIQELVDTEKSYVKDLSCLFELYLEPLQNETFLTQDEMESLFG
  SLPEMLEFQKVFLETLEDGISASSDFNTLETPSQFRKLLFSLGGSFLYYADHFKLYSGFCANHIKVQKVL
  ERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLKELVSLTDQESEEHYHLTEALKAMEK
  VASHINEMQKIYEDYGTVFDQLVAEQSGTEKEVTELSMGELLMHSTVSWLNPFLSLGKARKDLELTVFVF
  KRAVILVYKENCKLKKKLPSNSRPAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEI
  EGRPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSL
  KVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHPGLADFADNLIKES
  DILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPI
  KRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSVQSLTSVVSEECFYETESHGKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010927
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010927.2, NP_001010927.1
• RefSeq Size: 2668 bp
• RefSeq ORF: 1881 bp
• Locus ID: 26230
• Cytogenetics: 6q25.2-q25.3
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]