TIAM2 (NM_001010927) Human Untagged Clone

CAT#: SC301537

TIAM2 (untagged)-Human T-cell lymphoma invasion and metastasis 2 (TIAM2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIAM2
• Synonyms: STEF; TIAM-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001010927, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGGACCGCGGGAGAATCAGGATCCTCCTCCGAGGCCTCTGGCCCGCCACCTGTCTGATGCAGACC
  GCCTCCGCAAAGTCATCCAGGAGCTTGTGGACACAGAGAAGTCCTACGTGAAGGATTTGAGCTGCCTCTT
  TGAATTATACTTGGAGCCACTTCAGAATGAGACCTTTCTTACCCAAGATGAGATGGAGTCACTTTTTGGA
  AGTTTGCCAGAGATGCTTGAGTTTCAGAAGGTGTTTCTGGAGACCCTGGAGGATGGGATTTCAGCATCAT
  CTGACTTTAACACCCTAGAAACCCCCTCACAGTTTAGAAAATTACTGTTTTCCCTTGGAGGCTCTTTCCT
  TTATTACGCGGACCACTTTAAACTGTACAGTGGATTCTGTGCTAACCATATCAAAGTACAGAAGGTTCTG
  GAGCGAGCTAAAACTGACAAAGCCTTCAAGGCTTTTCTGGACGCCCGGAACCCCACCAAGCAGCATTCCT
  CCACGCTGGAGTCCTACCTCATCAAGCCGGTTCAGAGAGTGCTCAAGTACCCGCTGCTGCTCAAGGAGCT
  GGTGTCCCTGACGGACCAGGAGAGCGAGGAGCACTACCACCTGACGGAAGCACTAAAGGCAATGGAGAAA
  GTAGCGAGCCACATCAATGAGATGCAGAAGATCTATGAGGATTATGGGACCGTGTTTGACCAGCTAGTAG
  CTGAGCAGAGCGGAACAGAGAAGGAGGTAACAGAACTTTCGATGGGAGAGCTTCTGATGCACTCTACGGT
  TTCCTGGTTGAATCCATTTCTGTCTCTAGGAAAAGCTAGAAAGGACCTTGAGCTCACAGTATTTGTTTTT
  AAGAGAGCCGTCATACTGGTTTATAAAGAAAACTGCAAACTGAAAAAGAAATTGCCCTCGAATTCCCGGC
  CTGCACACAACTCTACTGACTTGGACCCATTTAAATTCCGCTGGTTGATCCCCATCTCCGCGCTTCAAGT
  CAGACTGGGGAATCCAGCAGGGACAGAAAATAATTCCATATGGGAACTGATCCATACGAAGTCAGAAATA
  GAAGGACGGCCAGAAACCATCTTTCAGTTGTGTTGCAGTGACAGTGAAAGCAAAACCAACATTGTTAAGG
  TGATTCGTTCTATTCTGAGGGAGAACTTCAGGCGTCACATAAAGTGTGAATTACCACTGGAGAAAACGTG
  TAAGGATCGCCTGGTACCTCTTAAGAACCGAGTTCCTGTTTCGGCCAAATTAGCTTCATCCAGGTCTTTA
  AAAGTCCTGAAGAATTCCTCCAGCAACGAGTGGACCGGTGAGACTGGCAAGGGAACCTTGCTGGACTCTG
  ACGAGGGCAGCTTGAGCAGCGGCACCCAGAGCAGCGGCTGCCCCACGGCTGAGGGCAGGCAGGACTCCAA
  GAGCACTTCTCCCGGGAAATACCCACACCCCGGCTTGGCAGATTTTGCCGACAATCTCATCAAAGAGAGT
  GACATCCTGAGCGATGAAGATGATGACCACCGTCAGACTGTGAAGCAGGGCAGCCCTACTAAAGACATCG
  AAATTCAGTTCCAGAGACTGAGGATTTCCGAGGACCCAGACGTTCACCCCGAGGCTGAGCAGCAGCCTGG
  CCCGGAGTCGGGTGAGGGTCAGAAAGGAGGAGAGCAGCCCAAACTGGTCCGGGGGCACTTCTGCCCCATT
  AAACGAAAAGCCAACAGCACCAAGAGGGACAGAGGAACTTTGCTCAAGGCGCAGATCCGTCACCAGTCCC
  TTGACAGTCAGTCTGAAAATGCCACCATCGACCTAAATTCTGTTCTAGAGCGAGAATTCAGTGTCCAGAG
  TTTAACATCTGTTGTCAGTGAGGAGTGTTTTTATGAAACAGAGAGCCACGGAAAATCATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001010927
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010927.2, NP_001010927.1
• RefSeq Size: 2668 bp
• RefSeq ORF: 1881 bp
• Locus ID: 26230
• Cytogenetics: 6q25.2-q25.3
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate 5' exon compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a.