PARP2 (NM_001042618) Human Tagged ORF Clone

CAT#: RG213586

PARP2 (GFP-tagged) - Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PARP2
• Synonyms: ADPRT2; ADPRTL2; ADPRTL3; ARTD2; pADPRT-2; PARP-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213586 representing NM_001042618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCGGCGGCGACGGAGCACCGGCGGCGGCAGGGCGAGAGCATTAAATGAAAGCAAAAGAGTTA
  ATAATGGCAACACGGCTCCAGAAGACTCTTCCCCTGCCAAGAAAACTCGTAGATGCCAGAGACAGGAGTC
  GAAAAAGATGCCTGTGGCTGGAGGAAAAGCTAATAAGGACAGGACAGAAGACAAGCAAGATGAATCTGTG
  AAGGCCTTGCTGTTAAAGGGCAAAGCTCCTGTGGACCCAGAGTGTACAGCCAAGGTGGGGAAGGCTCATG
  TGTATTGTGAAGGAAATGATGTCTATGATGTCATGCTAAATCAGACCAATCTCCAGTTCAACAACAACAA
  GTACTATCTGATTCAGCTATTAGAAGATGATGCCCAGAGGAACTTCAGTGTTTGGATGAGATGGGGCCGA
  GTTGGGAAAATGGGACAGCACAGCCTGGTGGCTTGTTCAGGCAATCTCAACAAGGCCAAGGAAATCTTTC
  AGAAGAAATTCCTTGACAAAACGAAAAACAATTGGGAAGATCGAGAAAAGTTTGAGAAGGTGCCTGGAAA
  ATATGATATGCTACAGATGGACTATGCCACCAATACTCAGGATGAAGAGGAAACAAAGAAAGAGGAATCT
  CTTAAATCTCCCTTGAAGCCAGAGTCACAGCTAGATCTTCGGGTACAGGAGTTAATAAAGTTGATCTGTA
  ATGTTCAGGCCATGGAAGAAATGATGATGGAAATGAAGTATAATACCAAGAAAGCCCCACTTGGGAAGCT
  GACAGTGGCACAAATCAAGGCAGGTTACCAGTCTCTTAAGAAGATTGAGGATTGTATTCGGGCTGGCCAG
  CATGGACGAGCTCTCATGGAAGCATGCAATGAATTCTACACCAGGATTCCGCATGACTTTGGACTCCGTA
  CTCCTCCACTAATCCGGACACAGAAGGAACTGTCAGAAAAAATACAATTACTAGAGGCTTTGGGAGACAT
  TGAAATTGCTATTAAGCTGGTGAAAACAGAGCTACAAAGCCCAGAACACCCATTGGACCAACACTATAGA
  AACCTACATTGTGCCTTGCGCCCCCTTGACCATGAAAGTTATGAGTTCAAAGTGATTTCCCAGTACCTAC
  AATCTACCCATGCTCCCACACACAGCGACTATACCATGACCTTGCTGGATTTGTTTGAAGTGGAGAAGGA
  TGGTGAGAAAGAAGCCTTCAGAGAGGACCTTCATAACAGGATGCTTCTATGGCATGGTTCCAGGATGAGT
  AACTGGGTGGGAATCTTGAGCCATGGGCTTCGAATTGCCCCACCTGAAGCTCCCATCACAGGTTACATGT
  TTGGGAAAGGAATCTACTTTGCTGACATGTCTTCCAAGAGTGCCAATTACTGCTTTGCCTCTCGCCTAAA
  GAATACAGGACTGCTGCTCTTATCAGAGGTAGCTCTAGGTCAGTGTAATGAACTACTAGAGGCCAATCCT
  AAGGCCGAAGGATTGCTTCAAGGTAAACATAGCACCAAGGGGCTGGGCAAGATGGCTCCCAGTTCTGCCC
  ACTTCGTCACCCTGAATGGGAGTACAGTGCCATTAGGACCAGCAAGTGACACAGGAATTCTGAATCCAGA
  TGGTTATACCCTCAACTACAATGAATATATTGTATATAACCCCAACCAGGTCCGTATGCGGTACCTTTTA
  AAGGTTCAGTTTAATTTCCTTCAGCTGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213586 representing NM_001042618
  Red=Cloning site Green=Tags(s)
  
  MAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDESV
  KALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRWGR
  VGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYATNTQDEEETKKEES
  LKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQ
  HGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTELQSPEHPLDQHYR
  NLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDLHNRMLLWHGSRMS
  NWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEANP
  KAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYIVYNPNQVRMRYLL
  KVQFNFLQLW
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042618
• ORF Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042618.1, NP_001036083.1
• RefSeq Size: 1865
• RefSeq ORF: 1713
• Locus ID: 10038
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008]