Ribonuclease Inhibitor (RNH1) (NM_203385) Human Tagged ORF Clone
CAT#: RG213589
- TrueORF®
RNH1 (GFP-tagged) - Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 4
"NM_203385" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | RNH1 |
Synonyms | RAI; RNH |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG213589 representing NM_203385
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTGGACATCCAGAGCCTGGACATCCAGTGTGAGGAGCTGAGCGACGCTAGATGGGCCGAGCTCC TCCCTCTGCTCCAGCAGTGCCAAGTGGTCAGGCTGGACGACTGTGGCCTCACGGAAGCACGGTGCAAGGA CATCAGCTCTGCACTTCGAGTCAACCCTGCACTGGCAGAGCTCAACCTGCGCAGCAACGAGCTGGGCGAT GTCGGCGTGCATTGCGTGCTCCAGGGCCTGCAGACCCCCTCCTGCAAGATCCAGAAGCTGAGCCTCCAGA ACTGCTGCCTGACGGGGGCCGGCTGCGGGGTCCTGTCCAGCACACTACGCACCCTGCCCACCCTGCAGGA GCTGCACCTCAGCGACAACCTCTTGGGGGATGCGGGCCTGCAGCTGCTCTGCGAAGGACTCCTGGACCCC CAGTGCCGCCTGGAAAAGCTGCAGCTGGAGTATTGCAGCCTCTCGGCTGCCAGCTGCGAGCCCCTGGCCT CCGTGCTCAGGGCCAAGCCGGACTTCAAGGAGCTCACGGTTAGCAACAACGACATCAATGAGGCTGGCGT CCGTGTGCTGTGCCAGGGCCTGAAGGACTCCCCCTGCCAGCTGGAGGCGCTCAAGCTGGAGAGCTGCGGT GTGACATCAGACAACTGCCGGGACCTGTGCGGCATTGTGGCCTCCAAGGCCTCGCTGCGGGAGCTGGCCC TGGGCAGCAACAAGCTGGGTGATGTGGGCATGGCGGAGCTGTGCCCAGGGCTGCTCCACCCCAGCTCCAG GCTCAGGACCCTGTGGATCTGGGAGTGTGGCATCACTGCCAAGGGCTGCGGGGATCTGTGCCGTGTCCTC AGGGCCAAGGAGAGCCTGAAGGAGCTCAGCCTGGCCGGCAACGAGCTGGGGGATGAGGGTGCCCGACTGC TGTGTGAGACCCTGCTGGAACCTGGCTGCCAGCTGGAGTCGCTGTGGGTGAAGTCCTGCAGCTTCACAGC CGCCTGCTGCTCCCACTTCAGCTCAGTGCTGGCCCAGAACAGGTTTCTCCTGGAGCTACAGATAAGCAAC AACAGGCTGGAGGATGCGGGCGTGCGGGAGCTGTGCCAGGGCCTGGGCCAGCCTGGCTCTGTGCTGCGGG TGCTCTGGTTGGCCGACTGCGATGTGAGTGACAGCAGCTGCAGCAGCCTCGCCGCAACCCTGTTGGCCAA CCACAGCCTGCGTGAGCTGGACCTCAGCAACAACTGCCTGGGGGACGCCGGCATCCTGCAGCTGGTGGAG AGCGTCCGGCAGCCGGGCTGCCTCCTGGAGCAGCTGGTCCTGTACGACATTTACTGGTCTGAGGAGATGG AGGACCGGCTGCAGGCCCTGGAGAAGGACAAGCCATCCCTGAGGGTCATCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213589 representing NM_203385
Red=Cloning site Green=Tags(s) MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGD VGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDP QCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCG VTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVL RAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISN NRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVE SVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_203385 |
ORF Size | 1383 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_203385.1, NP_976319.1 |
RefSeq Size | 1884 bp |
RefSeq ORF | 1386 bp |
Locus ID | 6050 |
Cytogenetics | 11p15.5 |
Gene Summary | 'Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC121744 | RNH1 (untagged)-Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 4 |
USD 760.00 |
|
RC213589 | RNH1 (Myc-DDK-tagged)-Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 4 |
USD 420.00 |
|
RC213589L3 | Lenti-ORF clone of RNH1 (Myc-DDK-tagged)-Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 4 |
USD 620.00 |
|
RC213589L4 | Lenti-ORF clone of RNH1 (mGFP-tagged)-Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 4 |
USD 620.00 |
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