ALDH1L1 (NM_012190) Human Tagged ORF Clone

CAT#: RG213720

ALDH1L1 (GFP-tagged) - Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALDH1L1
• Synonyms: 10-fTHF; 10-FTHFDH; FDH; FTHFD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213720 representing NM_012190
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTGCAGTGATTGGACAGAGCCTGTTTGGCCAGGAAGTTTACTGCCACCTGAGGAAGGAGGGCC
  ACGAAGTGGTGGGTGTGTTCACTGTTCCAGACAAGGATGGAAAGGCCGACCCCCTGGGTCTGGAAGCTGA
  GAAGGATGGAGTGCCGGTATTCAAGTACTCCCGGTGGCGTGCAAAAGGACAGGCTTTGCCTGATGTGGTG
  GCAAAATACCAGGCTTTGGGGGCCGAGCTCAACGTCCTGCCCTTCTGCAGCCAATTCATCCCCATGGAGA
  TAATCAGTGCCCCCCGGCATGGCTCCATCATCTATCACCCGTCACTGCTCCCTAGGCACCGAGGGGCCTC
  GGCCATCAACTGGACCCTCATTCACGGAGATAAGAAAGGGGGGTTTTCCATCTTCTGGGCGGATGATGGT
  CTGGACACCGGAGACCTGCTGCTGCAGAAGGAGTGTGAGGTGCTCCCGGACGACACCGTGAGCACGCTGT
  ACAACCGCTTCCTCTTCCCTGAAGGCATCAAAGGGATGGTGCAGGCCGTGAGGCTGATCGCTGAGGGCAA
  AGCCCCCAGACTCCCTCAGCCTGAGGAAGGAGCCACCTATGAGGGGATTCAGAAGAAGGAGACAGCCAAG
  ATCAACTGGGACCAGCCGGCAGAGGCCATTCACAACTGGATCCGCGGGAACGACAAGGTGCCGGGAGCCT
  GGACAGAGGCCTGTGAACAGAAACTGACATTTTTCAACTCAACGCTGAACACTTCAGGCCTGGTGCCCGA
  GGGAGACGCTTTGCCCATCCCAGGAGCCCATCGGCCAGGGGTGGTCACCAAAGCAGGACTCATCCTCTTT
  GGGAATGATGACAAAATGCTGCTGGTGAAGAATATTCAGCTGGAGGATGGCAAAATGATCCTGGCCTCGA
  ACTTCTTTAAGGGGGCAGCCAGCAGTGTCCTTGAGCTGACAGAGGCAGAGCTGGTTACTGCGGAGGCTGT
  GCGGAGTGTTTGGCAGCGGATCCTCCCCAAAGTCCTGGAGGTTGAAGACTCCACTGATTTCTTCAAGTCA
  GGGGCCGCGTCTGTGGACGTTGTGAGGCTGGTGGAGGAAGTGAAGGAGCTGTGTGATGGCCTGGAGTTAG
  AAAATGAAGATGTGTACATGGCATCCACCTTTGGGGACTTCATCCAGCTGTTAGTGAGGAAGCTGCGAGG
  GGACGATGAGGAGGGCGAGTGCAGCATTGACTACGTGGAAATGGCAGTGAACAAGCGCACTGTCCGCATG
  CCCCACCAGCTCTTCATTGGGGGGGAGTTCGTGGATGCCGAGGGCGCCAAGACCTCTGAGACCATCAATC
  CCACCGATGGAAGTGTCATCTGCCAGGTATCCCTGGCCCAAGTCACCGACGTCGACAAGGCAGTGGCCGC
  AGCCAAGGATGCCTTTGAGAATGGACGGTGGGGGAAGATCAGTGCGCGGGACCGGGGCCGGCTGATGTAC
  AGGTTGGCAGATCTCATGGAGCAGCACCAGGAGGAGCTGGCCACCATTGAGGCCCTGGATGCGGGTGCCG
  TCTACACGCTGGCCCTGAAGACCCACGTGGGCATGTCCATCCAGACCTTCCGCTACTTTGCTGGCTGGTG
  TGACAAGATCCAGGGCTCCACCATCCCCATCAACCAGGCCAGACCCAACCGCAACCTGACCTTGACCAGG
  AAGGAGCCTGTTGGGGTTTGTGGCATCATCATCCCCTGGAACTATCCCCTGATGATGCTGTCCTGGAAGA
  CAGCTGCCTGCCTGGCTGCCGGGAACACAGTGGTGATCAAGCCTGCTCAGGTGACCCCACTCACAGCCTT
  GAAGTTTGCAGAGCTGACATTAAAGGCCGGCATTCCCAAAGGTGTGGTTAACGTCCTCCCAGGATCTGGC
  TCCCTGGTCGGCCAGAGACTCTCAGACCATCCTGATGTGAGGAAAATCGGGTTCACAGGCTCCACAGAGG
  TGGGCAAGCACATCATGAAAAGCTGTGCCATAAGTAACGTGAAGAAGGTGTCCCTGGAACTGGGCGGGAA
  GTCACCCCTCATCATCTTTGCTGACTGTGACCTCAACAAGGCTGTGCAGATGGGGATGAGTTCTGTTTTC
  TTCAACAAAGGAGAGAATTGCATTGCAGCAGGCCGACTCTTTGTGGAGGACTCCATTCATGATGAGTTCG
  TGCGGAGAGTGGTAGAAGAGGTGCGGAAGATGAAGGTGGGCAACCCGCTGGACAGGGACACCGACCACGG
  GCCGCAGAATCACCATGCCCACCTTGTGAAGCTGATGGAGTACTGCCAGCATGGCGTGAAGGAAGGGGCC
  ACACTGGTCTGCGGCGGGAATCAGGTCCCTCGGCCAGGGTTCTTCTTTGAGCCAACTGTTTTCACAGACG
  TGGAAGACCACATGTTCATAGCCAAGGAGGAGTCCTTCGGGCCTGTCATGATCATCTCTCGGTTTGCTGA
  TGGGGACTTGGATGCCGTGCTGTCTCGGGCCAATGCCACGGAATTTGGCCTGGCTTCTGGTGTCTTCACC
  AGGGACATCAACAAGGCCCTGTATGTCAGTGACAAGCTCCAGGCAGGCACTGTGTTTGTCAACACGTACA
  ACAAGACCGACGTGGCCGCTCCCTTCGGAGGATTCAAACAGTCTGGATTTGGCAAAGATCTAGGAGAGGC
  GGCTCTGAACGAGTACCTGCGGGTCAAGACAGTGACCTTCGAATAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213720 representing NM_012190
  Red=Cloning site Green=Tags(s)
  
  MKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKYSRWRAKGQALPDVV
  AKYQALGAELNVLPFCSQFIPMEIISAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFSIFWADDG
  LDTGDLLLQKECEVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGKAPRLPQPEEGATYEGIQKKETAK
  INWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILF
  GNDDKMLLVKNIQLEDGKMILASNFFKGAASSVLELTEAELVTAEAVRSVWQRILPKVLEVEDSTDFFKS
  GAASVDVVRLVEEVKELCDGLELENEDVYMASTFGDFIQLLVRKLRGDDEEGECSIDYVEMAVNKRTVRM
  PHQLFIGGEFVDAEGAKTSETINPTDGSVICQVSLAQVTDVDKAVAAAKDAFENGRWGKISARDRGRLMY
  RLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGSTIPINQARPNRNLTLTR
  KEPVGVCGIIIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNVLPGSG
  SLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCAISNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVF
  FNKGENCIAAGRLFVEDSIHDEFVRRVVEEVRKMKVGNPLDRDTDHGPQNHHAHLVKLMEYCQHGVKEGA
  TLVCGGNQVPRPGFFFEPTVFTDVEDHMFIAKEESFGPVMIISRFADGDLDAVLSRANATEFGLASGVFT
  RDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRVKTVTFEY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012190
• ORF Size: 2706 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012190.2, NP_036322.2
• RefSeq Size: 3125
• RefSeq ORF: 2709
• Locus ID: 10840
• Domains: aldedh, formyl_transf, formyl_trans_C
• Protein Families: Druggable Genome
• Protein Pathways: One carbon pool by folate
• Gene Summary: The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]