LDB3 (NM_001080114) Human Tagged ORF Clone

CAT#: RG213766

LDB3 (GFP-tagged) - Human LIM domain binding 3 (LDB3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LDB3
• Synonyms: CMD1C; CMH24; CMPD3; CYPHER; LDB3Z1; LDB3Z4; LVNC3; MFM4; ORACLE; PDLIM6; ZASP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213766 representing NM_001080114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTACAGTGTGACCCTGACTGGGCCCGGGCCCTGGGGCTTCCGTCTGCAGGGGGGCAAGGACTTCA
  ACATGCCCCTCACTATCTCCCGGATCACACCAGGCAGCAAGGCAGCCCAGTCCCAGCTCAGCCAGGGTGA
  CCTCGTGGTGGCCATTGACGGCGTCAACACAGACACCATGACCCACCTGGAAGCCCAGAACAAGATCAAG
  TCTGCCAGCTACAACTTGAGCCTCACCCTGCAGAAATCAAAGCGTCCCATTCCCATCTCCACGACAGCAC
  CTCCAGTCCAGACCCCTCTGCCGGTGATCCCTCACCAGAAGGTGGTAGTCAACTCTCCAGCCAACGCCGA
  CTACCAGGAACGCTTCAACCCCAGTGCCCTGAAGGACTCGGCCCTGTCCACCCACAAGCCCATCGAGGTG
  AAGGGGCTGGGCGGCAAGGCCACCATCATCCATGCGCAGTACAACACGCCCATCAGCATGTATTCCCAGG
  ATGCCATCATGGATGCCATCGCTGGGCAGGCCCAAGCCCAAGGCAGTGACTTCAGTGGGAGCCTCCCTAT
  TAAGGACCTTGCCGTAGACAGCGCCTCTCCCGTCTACCAGGCTGTGATTAAGAGCCAGAACAAGCCAGAA
  GATGAGGCTGACGAGTGGGCACGCCGTTCCTCCAACCTGCAGTCTCGCTCCTTCCGCATCCTGGCCCAGA
  TGACGGGGACAGAATTCATGCAAGACCCTGATGAAGAAGCTCTGCGAAGGTCAAGGCCCCAGGCCTCTTC
  CTACAGCCCCGCAGTGGCCGCCTCTTCAGCACCTGCCACCCACACCAGCTACAGTGAGGGCCCCGCCGCC
  CCTGCACCCAAGCCCCGGGTTGTCACCACTGCCAGCATCCGGCCTTCTGTCTACCAGCCAGTGCCTGCAT
  CTACCTACAGCCCGTCCCCAGGGGCCAATTACAGTCCCACTCCCTACACCCCCTCCCCTGCCCCTGCCTA
  CACCCCCTCCCCTGCCCCTGCCTACACCCCCTCACCTGTCCCCACCTACACTCCATCCCCAGCACCAGCC
  TATACCCCCTCACCTGCCCCCAACTATAACCCTGCACCCTCGGTGGCCTACAGCGGGGGCCCTGCGGAGC
  CTGCCAGCCGTCCACCCTGGGTGACAGATGATAGCTTCTCCCAGAAGTTTGCCCCGGGCAAGAGCACCAC
  CTCCATCAGCAAGCAGACCCTGCCCCGGGGAGGCCCAGCCTACACCCCAGCGGGTCCTCAGGTGCCACCA
  CTTGCCAGGGGGACCGTCCAGAGGGCTGAGCGATTCCCAGCCAGCAGCCGGACTCCACTCTGCGGTCACT
  GCAACAATGTCATCCGGGGCCCATTTCTGGTAGCCATGGGCCGTTCTTGGCACCCTGAAGAGTTCACCTG
  TGCCTACTGCAAGACTTCCCTGGCAGATGTGTGCTTTGTGGAAGAGCAGAACAACGTTTACTGTGAGCGA
  TGTTATGAGCAATTCTTTGCCCCGCTGTGTGCCAAGTGCAACACCAAAATTATGGGGGAAGTAATGCATG
  CCTTGAGACAGACATGGCACACCACCTGCTTCGTCTGTGCGGCCTGCAAGAAGCCTTTTGGGAACAGCCT
  CTTCCACATGGAAGACGGGGAGCCCTACTGCGAGAAAGACTACATCAATCTGTTCAGCACCAAGTGCCAT
  GGCTGCGATTTCCCCGTGGAGGCTGGCGACAAGTTTATCGAAGCCCTGGGCCACACTTGGCACGACACCT
  GCTTCATTTGCGCAGTCTGCCATGTGAATCTGGAGGGGCAGCCGTTCTACTCCAAGAAGGACAGACCCCT
  GTGCAAGAAGCACGCACACACCATCAACTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213766 representing NM_001080114
  Red=Cloning site Green=Tags(s)
  
  MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIK
  SASYNLSLTLQKSKRPIPISTTAPPVQTPLPVIPHQKVVVNSPANADYQERFNPSALKDSALSTHKPIEV
  KGLGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGSDFSGSLPIKDLAVDSASPVYQAVIKSQNKPE
  DEADEWARRSSNLQSRSFRILAQMTGTEFMQDPDEEALRRSRPQASSYSPAVAASSAPATHTSYSEGPAA
  PAPKPRVVTTASIRPSVYQPVPASTYSPSPGANYSPTPYTPSPAPAYTPSPAPAYTPSPVPTYTPSPAPA
  YTPSPAPNYNPAPSVAYSGGPAEPASRPPWVTDDSFSQKFAPGKSTTSISKQTLPRGGPAYTPAGPQVPP
  LARGTVQRAERFPASSRTPLCGHCNNVIRGPFLVAMGRSWHPEEFTCAYCKTSLADVCFVEEQNNVYCER
  CYEQFFAPLCAKCNTKIMGEVMHALRQTWHTTCFVCAACKKPFGNSLFHMEDGEPYCEKDYINLFSTKCH
  GCDFPVEAGDKFIEALGHTWHDTCFICAVCHVNLEGQPFYSKKDRPLCKKHAHTINL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080114
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001080114.1, NP_001073583.1
• RefSeq Size: 4978
• RefSeq ORF: 1854
• Locus ID: 11155
• Gene Summary: This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. [provided by RefSeq, Jan 2010]