PHF21A (NM_001101802) Human Tagged ORF Clone

CAT#: RG213929

PHF21A (GFP-tagged) - Human PHD finger protein 21A (PHF21A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHF21A
• Synonyms: BHC80; BM-006
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213929 representing NM_001101802
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGCAGACTCTACAGGAGGCTCTTAAAGTGGAAATTCAGGTTCACCAGAAACTGGTTGCTCAAA
  TGAAGCAGGATCCACAGAATGCTGACTTAAAGAAACAGCTTCATGAACTCCAAGCCAAAATCACAGCTTT
  GAGTGAGAAACAGAAAAGAGTAGTTGAACAGCTACGGAAGAACCTGATAGTAAAGCAAGAACAACCGGAC
  AAGTTCCAAATACAGCCATTGCCACAATCTGAAAACAAACTACAAACAGCACAGCAGCAACCACTACAGC
  AACTACAACAACAGCAGCAGTACCACCACCACCACGCCCAGCAGTCAGCTGCAGCCTCTCCCAACCTGAC
  TGCTTCACAGAAGACTGTAACTACAGCTTCTATGATTACCACAAAGACACTACCTCTCGTCTTGAAAGCA
  GCAACTGCGACCATGCCTGCCTCTGTGGTGGGCCAGAGACCTACCATTGCTATGGTGACCGCCATCAACA
  GTCAGAAGGCTGTGCTCAGCACTGATGTGCAGAACACACCAGTCAACCTCCAGACGTCTAGTAAGGTCAC
  TGGGCCTGGGGCAGAGGCTGTCCAAATTGTGGCAAAAAACACAGTCACTCTGGTTCAGGCAACACCTCCT
  CAGCCCATCAAAGTACCACAGTTTATCCCCCCTCCTAGACTCACTCCACGTCCAAACTTTCTTCCACAGG
  TTCGACCCAAGCCTGTGGCCCAGAATAACATTCCTATTGCCCCAGCACCACCTCCCATGCTCGCAGCTCC
  TCAGCTTATCCAGAGGCCCGTCATGCTGACCAAGTTCACCCCCACAACCCTTCCCACATCCCAGAATTCC
  ATCCACCCCGTCCGTGTCGTCAATGGGCAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGCTCA
  CCAGCATTGTGATAGCTACTCCAGGGACCAGACTCGCTGGACCTCAAACTGTACAGCTTAGCAAGCCAAG
  TCTTGAAAAACAGACAGTTAAATCTCACACAGAAACAGATGAGAAACAAACAGAGAGCCGCACCATCACC
  CCACCTGCTGCACCCAAACCAAAACGGGAGGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAGGGT
  TGGTAACACATGACCATCTAGAAGAAATCCAAAGCAAGAGGCAAGAGCGAAAAAGAAGAACAACAGCAAA
  TCCGGTCTACAGTGGAGCAGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTGACATACCTAAACAGCACA
  ATGCACCCTGGGACCCGGAAGAGAGGTCGTCCTCCAAAATACAATGCAGTGCTGGGGTTTGGAGCCCTTA
  CCCCAACATCCCCCCAATCCAGTCATCCTGACTCCCCTGAAAATGAAAAGACAGAGACCACATTCACTTT
  CCCTGCACCTGTTCAGCCTGTGTCCCTGCCCAGCCCCACCTCCACAGACGGTGATATTCATGAGGATTTT
  TGCAGCGTTTGCAGAAAAAGTGGCCAGTTACTGATGTGCGACACATGTTCCCGTGTATATCATTTGGACT
  GCTTAGACCCCCCTCTGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATGCT
  GAAGAAGGAAGAAGCAATTCCATGGCCTGGAACTTTAGCAATTGTTCATTCCTATATTGCCTACAAAGCA
  GCAAAAGAAGAAGAGAAACAGAAGTTACTTAAATGGAGTTCAGATTTAAAACAAGAACGAGAACAACTAG
  AGCAAAAGGTGAAACAGCTCAGCAATTCCATAAGTAAATGCATGGAAATGAAGAACACCATCCTGGCCCG
  GCAGAAGGAGATGCACAGCTCCCTGGAGAAGGTAAAACAGCTGATTCGCCTCATCCACGGCATCGACCTC
  TCCAAACCTGTAGACTCTGAGGCCACTGTGGGGGCCATCTCCAATGGCCCGGACTGCACCCCCCCTGCCA
  ATGCCGCCACCTCCACGCCGGCCCCTTCCCCCTCCTCCCAGAGCTGCACAGCGAACTGTAACCAGGGGGA
  AGAGACTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213929 representing NM_001101802
  Red=Cloning site Green=Tags(s)
  
  MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLIVKQEQPD
  KFQIQPLPQSENKLQTAQQQPLQQLQQQQQYHHHHAQQSAAASPNLTASQKTVTTASMITTKTLPLVLKA
  ATATMPASVVGQRPTIAMVTAINSQKAVLSTDVQNTPVNLQTSSKVTGPGAEAVQIVAKNTVTLVQATPP
  QPIKVPQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQNS
  IHPVRVVNGQTATIAKTFPMAQLTSIVIATPGTRLAGPQTVQLSKPSLEKQTVKSHTETDEKQTESRTIT
  PPAAPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNST
  MHPGTRKRGRPPKYNAVLGFGALTPTSPQSSHPDSPENEKTETTFTFPAPVQPVSLPSPTSTDGDIHEDF
  CSVCRKSGQLLMCDTCSRVYHLDCLDPPLKTIPKGMWICPRCQDQMLKKEEAIPWPGTLAIVHSYIAYKA
  AKEEEKQKLLKWSSDLKQEREQLEQKVKQLSNSISKCMEMKNTILARQKEMHSSLEKVKQLIRLIHGIDL
  SKPVDSEATVGAISNGPDCTPPANAATSTPAPSPSSQSCTANCNQGEETK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101802
• ORF Size: 2040 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001101802.1, NP_001095272.1
• RefSeq Size: 7332
• RefSeq ORF: 2043
• Locus ID: 51317
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]). [supplied by OMIM, Nov 2010]